Preparation method and application of bionic multifunctional composite sponge dressing

A composite sponge and multi-functional technology, applied in medical science, absorbent pads, bandages, etc., can solve the problems of low release efficiency of active ingredients, hindering the release of active ingredients, and decreased healing effect of dressings, etc., to promote collagen deposition, inhibit Bacterial infection, the effect of accelerating the wound healing process

Inactive Publication Date: 2021-08-13
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using a composite substrate to support the hydrogel hinders the release of the active ingredients wrapped in the hydrogel, reducing the healing effect of the dressing
[0005] In summary, hydrogel bioactive dressings generally have poor mechanical properties and cannot be well attached to skin wounds. During use, there are low release efficiency of active ingredients, low strength, easy to fall off, and easy to shift. And other issues

Method used

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  • Preparation method and application of bionic multifunctional composite sponge dressing
  • Preparation method and application of bionic multifunctional composite sponge dressing
  • Preparation method and application of bionic multifunctional composite sponge dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:

[0040] (1) Preparation of hyaluronic acid grafted with dopamine:

[0041] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1 to 2 hours, and adjust the pH of the solution to 5; control the reaction temperature at 5°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 2 h; vacuumed for 25 min, added 2 g of dopamine hydrochloride, stirred thoroughly for 30 min, vacuumed again for 25 min, and reacted at 6°C for 30 h; (pH=4) dialysis, then dialysis with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;

[0042] (2) Use tyrosinase to realize cross-linking:

[0043] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) gelatin solu...

Embodiment 2

[0047] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:

[0048] (1) Preparation of hyaluronic acid grafted with dopamine:

[0049] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1-2 hours, and adjust the pH of the solution to 4.5; control the reaction temperature at 5°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 1 h; vacuumed for 20 min, added 4 g of dopamine hydrochloride, stirred thoroughly for 25 min, vacuumed again for 20 min, and reacted at 6°C for 26 h; (pH=3) dialysis, then dialyzed with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;

[0050] (2) Use tyrosinase to realize cross-linking:

[0051] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) gelatin solut...

Embodiment 3

[0055] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:

[0056] (1) Preparation of hyaluronic acid grafted with dopamine:

[0057] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1 to 2 hours, and adjust the pH of the solution to 5.5; control the reaction temperature at 4°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 1 h; vacuumed for 15 min, added 3.80 g of dopamine hydrochloride, stirred thoroughly for 20 min, vacuumed again for 15 min, and reacted at 4°C for 24 h; HCl (pH=3.5) dialysis, then dialysis with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;

[0058] (2) Use tyrosinase to realize cross-linking:

[0059] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) g...

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Abstract

The invention discloses a preparation method of a bionic multifunctional composite sponge dressing. The method specifically comprises the following steps that firstly, a hyaluronic acid solution is enabled to react with dopamine to obtain a dopamine-grafted hyaluronic acid solution; secondly, the dopamine-grafted hyaluronic acid solution obtained in the previous step is mixed with a gelatin solution according to different volume ratios, then tyrosinase is added into a mixed system, and freeze-drying is conducted to obtain a composite dressing matrix; and finally, the prepared composite dressing matrix is soaked in a polyhexamethylene biguanide solution (PHMB), a titanium dioxide nano-particle solution (TiO2NPs) and a mixed solution of polyhexamethylene biguanide and titanium dioxide nano-particles, drying is conducted after ultrasonic concussion, and three kinds of solution-loaded composite sponge dressings are obtained accordingly. The obtained composite sponge dressing not only has the strong mechanical property and good adhesion, but also has the good antibacterial property, biocompatibility and the strong hemostatic ability.

Description

technical field [0001] The invention belongs to the technical field of biomedical dressings, and in particular relates to a preparation method and application of a bionic multifunctional composite sponge dressing. Background technique [0002] Skin is an important physical and biological barrier for humans and animals to resist adverse reactions such as pathogens, toxins, bacteria, and allergens. The skin not only plays a key role in sensory function, thermoregulation, homeostasis, maintenance of electrolyte balance, etc., but also prevents dehydration of internal tissues and participates in the regulation of many metabolic processes. However, in daily life, the skin is easily damaged by the outside world, forming trauma. [0003] Over the past few decades, extensive advances in biomedicine have significantly improved the treatment of skin wounds. Bioactive dressings, as a kind of skin wound dressing, firstly need to have good adhesion performance, so that the dressing can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/42A61L15/32A61L15/38A61L15/28A61L15/26A61L15/18A61L15/44A61L15/46
CPCA61L15/425A61L15/28A61L15/32A61L15/38A61L15/46A61L15/44A61L15/18A61L15/26A61L2300/102A61L2300/206A61L2300/404A61L2300/412A61L2300/204C08L5/08C08L89/00
Inventor 李兢思石贤爱杨建民
Owner FUZHOU UNIV
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