Integrated micro-nanofiber/hydrogel double network artificial blood vessel and its preparation method

Abstract

The present invention relates to an integrated micro-nanofiber / hydrogel double-network artificial blood vessel and its manufacturing method. The artificial blood vessel has three functional layers, the inner and outer layers are electrospun layers, and the middle layer is micro-nanofiber / hydrogel. The hydrogel double network is composed of interwoven micro-nanofiber three-dimensional network and hydrogel network. The micro-nanofiber three-dimensional network is constructed of micro-nanofibers with a high aspect ratio and more than 99% of the fibers are in a monodisperse state; The method is as follows: (1) make an electrospinning solution with high biocompatibility; (2) spin the electrospinning solution prepared in step (1) on the mold; first spin for 3 to 5 hours to form the inner layer of the blood vessel , and then spray the hydrogel precursor powder while spinning to form the middle layer of blood vessels, and finally stop spraying and continue spinning for 3 to 10 minutes to form the outer layer of blood vessels; (3) impregnate the product of step (2) into an excess In the aqueous solution of the agent, the product is obtained after removing the mold. The invention is integrally formed, has good mechanical properties, and is not easy to fall apart during application.

Description

Technical field [0001] The present invention belongs to artificial blood vessel technology, and it is related to an integrated molding micro-nanofiber / hydrogel dual network artificial blood vessel and method. Background technique [0002] Artificial blood vessel transplantation is an effective treatment for replacing disease and helping blood vessel regeneration. The human vascular structure includes three-layer structure: inner membrane, medium film and outer membrane. Since each structure is composed of different cells, the ideal artificial blood vessel should be designed to have three different functional layers, with a suitable degradation rate, and promote the gradual regeneration of blood vessels. The existing design idea is mostly prepared by the micro-nanofiber and hydrogel composite of the imitation extracellular matrix, and there are several preparation methods: [0003] 1. Microbial fiber impregnated hydrogel solution is formed, and then multilayer composite, such as...

AI Technical Summary

Problems solved by technology

[0003] 1. Micro-nano fibers are impregnated with hydrogel solution to form, and then multi-layer compounded, such as patent CN201910023769.8, the fiber layer is immersed in the hydrogel solution, and then the hydrogel is bonded to the fiber layer by freezing / thawing method to form Preform, repeat the above steps to change the loading capacity of the hydrogel, and then use the above-mentioned preform loaded with hydrogel to receive electrospinning to form the desired product. The artificial blood vessel prepared by this method needs to be repeated many times The steps of impregnation, coating, freezing and thawing will cause certain damage to the mechanical properties of the fiber layer, and the bonding force between the fiber and the hydrogel is not enough, and the three-layer structure is easy to detach in practical applications.

[0004] 2. Hydrogel is formed in situ on the surface of the micro-nano fiber, and then multi-layer composite is carried out. For example, the method adopted in patent CN201911054424.5 is to place the fiber layer in the mold, pour the hydrogel precursor on the surface of the fiber layer, and use the initiator And self-assembly to form a hydrogel. This method needs to add an initiator when preparing blood vessels, and the forming process needs to modify the hydrogel, which is complicated to operate. In addition, when the composite is repeated, the binding force between the fiber layer and the hydrogel is insufficient. , may cause structural damage when applied

[0005] Therefore, it is urgent to study a method that can solve the problem of insufficient binding force between the above-mentioned fiber layer and hydrogel.