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Rice endophytic Bacillus velezensis and application thereof

A technology of Bacillus velesi and Bacillus, applied in the field of microorganisms, can solve problems such as rotten seeds and decreased yield, and achieve the effects of simple process, promotion of germination, and easy expansion of cultivation.

Pending Publication Date: 2021-08-20
HUAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Dicky zea invaded before the seeds had grown into seedlings, causing seed rot and subsequent drop in yield, so it was too late to remedy the remaining seedlings by injecting Bacillus Velez
[0004] At present, there are few domestic and foreign reports on the prevention and control of D.zeae invading rice seeds and causing rotten seeds and buds

Method used

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  • Rice endophytic Bacillus velezensis and application thereof
  • Rice endophytic Bacillus velezensis and application thereof
  • Rice endophytic Bacillus velezensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation and Screening of Bacterial Strains

[0035] Collection of healthy rice plants: The healthy plants of the rice variety "Guangjing Lizhan" resistant to the Dickyella corneum EC1 strain were collected at the Wushan Experimental Base of the Rice Research Institute of the Guangdong Academy of Agricultural Sciences on May 20, 2019, and were stored in bags and brought back to the experiment Laboratory for isolation of endophytic strains.

[0036] Isolation of endophytic strains: Take healthy rice roots, stems and leaves, wash the surface briefly with running water, cut into suitable small pieces with sterilized scissors, soak in 75% ethanol for 3 minutes, put them in 2% sodium hypochlorite for 5 minutes, and then use Soak in sterile water for 6 minutes, then rinse with sterile water for 6 times, and use the last sterile water coating as a control. Use sterilized filter paper to blot dry the water on the surface of the washed rice tissue block, put it into...

Embodiment 2

[0040] Embodiment 2: biological characteristic analysis

[0041] Use an inoculation loop to pick a single colony of J17-4 and streak it on LB medium, culture at 28°C for 24 hours, and observe the growth of the colony. The bacterial colony on the LB medium is off-white, raised in the middle, opaque, and the edges are irregular ( figure 2 ), showing diffuse turbidity in the liquid medium.

Embodiment 3

[0042] Embodiment 3: culture medium screening and antibiotic susceptibility test

[0043]The present invention has taken NYD (beef extract 8g, yeast extract 3g, glucose 1g, distilled water 1000mL), YPG (yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000mL), YPD (yeast extract 10g, tryptone 20g, Glucose 20g, distilled water 1000mL), LB (yeast extract 5g, peptone 20g, NaCl 20g, distilled water 1000mL), LB broth (LB broth medium 30g, distilled water 1000mL) and tryptone (tryptone liquid culture Base 30g, distilled water 1000mL) on the antibacterial effect of J17-4 against Dicky zea zea in 6 kinds of media.

[0044] the result shows( image 3 ), the diameter of the inhibition zone of strain J17-4 in tryptone soy medium was the largest. Therefore, the study concluded that the optimum medium for strain J17-4 was tryptone.

[0045] The experiment also analyzed the sensitivity of J17-4 to antibiotics, and the results showed that the endophytic bacteria had strong res...

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Abstract

The invention relates to a Bacillus velezensis J17-4 strain capable of reducing the inhibition effect of Dickeya zeae on the germination of plant seeds, and an application of the Bacillus velezensis J17-4 strain. The J17-4 strain is preserved in the CCTCC(China Center for Type Culture Collection) on July 15, 2020, and the preservation number is CCTCC NO: M 2020318. The J17-4 strain can effectively reduce the inhibition effect of the Dickeya zeae on plant seed germination and promote the seed germination, especially rice seeds; and meanwhile, the Bacillus velezensis J17-4 also has a remarkable inhibition effect on the Dickeya zeae, and can be used for preventing and treating rice bacterial foot rot. A bacterial suspension of the strain can be used for preparing a microbial preparation, and has a very high application value in aspects of reducing the inhibition effect of the Dickeya zeae on plant seed germination and preventing and treating rice bacterial foot rot.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to Bacillus velesi J17-4 and applications thereof. Background technique [0002] Dickeya zeae is widely distributed in rice-growing areas at home and abroad, and can cause serious rice diseases. D.zeae can invade during the germination stage of rice seeds, causing rotten seeds and buds. At the same time, it can also invade at different growth stages of rice, causing the base of the stem to rot, reduce tillers, and produce white ears and grains. The loss of harvest area poses a great threat to the high and stable yield of rice. The disease caused by the infection of Dickyella maize has seriously reduced the yield of food crops and seriously threatened food security. The comprehensive control technology based on chemical control is currently the main measure to control the crops caused by Dickyella maize. However, the extensive use of chemical pesticides causes environ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01C1/00A01N63/22A01P1/00C12R1/07
CPCC12N1/20A01C1/00A01N63/22
Inventor 施祖荣王庆伟洪婉雯黄江华
Owner HUAINAN NORMAL UNIV
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