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Composition for inhibiting generation of melanin and applications thereof

A technology for inhibiting melanin and composition, which is applied in the field of daily chemicals, can solve the problems of single action of arbutin, skin irritation, and easy discoloration of kojic acid, etc., and achieve the effect of inhibiting the formation of melanin, activating the function, and reducing the formation of melanin

Pending Publication Date: 2021-08-31
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, hydroquinone is very irritating to the skin; kojic acid is easy to change color and is also irritating to the skin; arbutin has a single effect and poor effect, etc.

Method used

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  • Composition for inhibiting generation of melanin and applications thereof
  • Composition for inhibiting generation of melanin and applications thereof
  • Composition for inhibiting generation of melanin and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0076] The Bordeaux leaf extract was diluted with phosphate buffered saline to obtain 6 groups of test solutions with different concentrations (final concentrations), which were filtered with a 0.22 μm filter head. When performing the cell viability test, the mass concentration of the Bordeaux leaf extract is shown in Table 1 below.

[0077] Cell Viability Test

[0078]CCK-8 reagent contains WST-8: chemical name 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfone Acid phenyl)-2H-tetrazole monosodium salt, which is reduced to highly water-soluble Yellow formazan product, the amount of formazan produced is directly proportional to the number of viable cells. The light absorption value was measured at a wavelength of 450nm by an enzyme-linked immunosorbent assay instrument, which can indirectly reflect the number of living cells. This method has been widely used in the activity detection of some biologically active factors, large-scale antitumor drug screening, ce...

Embodiment 7-12

[0086] The Bordeaux leaf extract was diluted with phosphate buffered saline to obtain 6 groups of test solutions with different concentrations (final concentrations), which were filtered with a 0.22 μm filter head. When performing the cell viability test, the mass concentration of the Bordeaux leaf extract is shown in Table 2 below.

[0087] Melanocyte norepinephrine receptor inhibition test

[0088] When norepinephrine activates melanocyte β2-norepinephrine receptors, it generates cAMP via adenylyl cyclase (AC) activation. cAMP in turn activates PKA, which activates the gene expression of MITF through the phosphorylation of CREB. Finally, by interacting with TYR, TRP-1 and TRP-2 promoter regions, MITF gene is transcribed to produce melanin-related proteins. Therefore, detecting the concentration of intracellular cAMP can reflect the activation degree of β2-norepinephrine receptor.

[0089] Dilute melanoma B16 cells to 2.5*10 4 cells / ml, transfer the cell suspension to a...

Embodiment 13-17

[0098] Bordeaux leaf extract and 3-o-ethyl ascorbic acid were diluted with phosphate buffer to obtain 5 groups of test solutions with different concentrations (final concentrations). When testing, the mass concentrations of Bordeaux leaf extract and 3-o-ethyl ascorbic acid are shown in Table 3 below.

[0099] B16 cell melanin change rate test

[0100] Dilute melanoma B16 cells to 2.5*10 4 cells / ml, move the cell suspension to a 9cm culture dish, and add 100 μl of the cell suspension to each well of a flat-bottomed 96-well plate with a multi-channel pipette. The culture plate was placed in a 5% carbon dioxide incubator and incubated in a humid environment at 37° C. for 24 hours, and the Bordeaux leaf extract and 3-o-ethyl ascorbic acid (according to the mass concentration in Table 3) were added. After acting for 4 hours, add 10*10 -9 mol / L norepinephrine and 1*10 -6 mol / L α-MSH, and after 48 hours, the culture medium was aspirated, digested with 0.25% trypsin, centrifuged...

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Abstract

The invention belongs to the field of chemicals for daily use, and specifically provides a composition for inhibiting the generation of melanin and applications thereof. The provided composition includes a peumus boldus leaf extract, ascorbic acid and derivatives thereof. Through the collocation of the peumus boldus leaf extract, the ascorbic acid and derivatives thereof, the composition may have a synergistic effect, and can well inhibit the generation of the melanin. Whitening emulsion prepared by using the composition can reduce the generation of the melanin, achieve a whitening effect, increase the water content of cuticula, and block water loss in the deep layer of the skin.

Description

technical field [0001] The invention relates to the field of daily chemicals, in particular to a composition for inhibiting melanin production and its application. Background technique [0002] Melanin is mainly produced by melanocytes in the basal layer of human skin epidermis, which can reduce the damage of ultraviolet rays to the skin. The content and distribution of melanin determine the color of the skin. However, the abnormal accumulation of melanin in the basal layer will cause hyperpigmentation and cause melasma, freckles, age spots, etc., affecting people's quality of life. With the deepening of research on melanin biosynthesis, researchers found that tyrosinase plays an important role in melanin biosynthesis. It is the main rate-limiting enzyme in the process of converting tyrosine and dopa to melanin. Its overexpression It is an important cause of hyperpigmentation. Therefore, inhibiting the activity of tyrosinase can effectively block the biosynthesis reaction...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9783A61K8/67A61Q19/02A61Q19/00
CPCA61K8/9783A61K8/676A61Q19/02A61Q19/00A61K2800/592
Inventor 杨登亮周雨叶黄晓园林盛杰李传茂邓慧张伟杰曾伟丹张楚标
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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