A Broad Spectrum Escherichia coli Phage and Its Application

A technology of Escherichia coli and bacteriophage, applied in the direction of bacteriophage, virus/phage, application, etc., can solve the problems of medical and health industry crisis, increased expenditure and other problems, and achieve the effect of wide host range, excellent strain resources, and good application development prospects.
CN113337480BActive Publication Date: 2022-05-17HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2022-05-17

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a broad-spectrum colibacillus phage and its application. The Escherichia coli phage WXLECP008 (Escherichia coli phage, WXLECP008) provided by the present invention is a virulent phage, which has tolerance at pH 4 to pH 10 and at -80°C to 65°C, cultivated for 8 hours under the condition of MOI=0.1, and its titer is 3.5×10 10 pfu / mL. The Escherichia coli phage provided by the invention is a broad-spectrum phage with a wide cleavage range, has high titer and high safety, can reproduce in large quantities in non-pathogenic host bacteria, and can be used for large-scale fermentation production. The phage described in the invention can provide excellent bacterial strain resources for the development of phage therapy, has good application and development prospects, and is suitable for popularization and application.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a broad-spectrum colibacillus phage and its application. Background technique

[0002] Escherichia coli (Escherichia coli), commonly known as Escherichia coli, has been regarded as a component of normal intestinal flora for quite a long time and is considered to be a non-pathogenic bacterium. It was not until the middle of the 20th century that it was recognized that some special serotypes of Escherichia coli were pathogenic to humans and animals, often causing severe diarrhea and sepsis. Therefore, most E. coli are generally regarded as opportunistic pathogens. According to different biological characteristics, pathogenic Escherichia coli can be divided into 6 categories: enteropathogenic Escherichia coli (EPEC), enterotoxigenic Escherichia coli (ETEC), enteroinvasive Escherichia coli (EIEC), enterohaemorrhagic Escherichia coli (EHEC), enteroadhesive Escherichia coli (...

Claims

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