Polydopamine perfluorohexane nanoliposome and preparation method and application thereof

A nanoliposome, perfluorohexane technology, applied in the field of nanomaterials and biomedical materials, can solve the problem of ferroptosis of tumor cells

Active Publication Date: 2021-09-07
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no nanosystem that can solve the problem of tumor hypoxia and simultaneously trigger ferroptosis of tumor cells has been publicly reported.

Method used

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  • Polydopamine perfluorohexane nanoliposome and preparation method and application thereof
  • Polydopamine perfluorohexane nanoliposome and preparation method and application thereof
  • Polydopamine perfluorohexane nanoliposome and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A preparation method of polydopamine perfluorohexane nano liposomes, comprising the steps of:

[0034] (1) Preparation of perfluorohexane (PFH) nanoemulsion: Add 400 μL of perfluorohexane into 6 mL of ultrapure water, and perform ultrasonic emulsification and dispersion with an ultrasonic crusher (200W, 2s on, 1s off, total time 6min) into a uniform nanoemulsion;

[0035](2) Preparation of perfluorohexane-polydopamine (PFH-PDA) nanoparticles: Add 400 μL ammonia water and 4 mL ethanol solution to the 6 mL perfluorohexane emulsion in step (1), stir magnetically (100 rpm / min) for 30 min, and use Add 80 mg of dopamine hydrochloride and 10 mL of ultrapure water to the above blended solution, stir at room temperature for 24 hours, centrifuge for 15 minutes at a speed of 10,000 rpm, disperse the sediment at the bottom of the centrifuge tube with ultrapure water, centrifuge again, and repeat 3 times to obtain the described Perfluorohexane-polydopamine nanoparticles;

[0036] ...

Embodiment 2

[0041] A preparation method of polydopamine perfluorohexane nano liposomes, comprising the steps of:

[0042] (1) Preparation of perfluorohexane (PFH) nanoemulsion: Add 200 μL of perfluorohexane into 3 mL of ultrapure water, and perform ultrasonic emulsification and dispersion with an ultrasonic crusher (200W, 2s on, 1s off, total time 3min) into a uniform nanoemulsion;

[0043] (2) Preparation of perfluorohexane-polydopamine (PFH-PDA) nanoparticles: Add 200 μL ammonia water and 2 mL ethanol solution to the 6 mL perfluorohexane emulsion in step (1), stir magnetically (100 rpm / min) for 30 min, and use Add 40 mg of dopamine hydrochloride and 5 mL of ultrapure water to the above blended solution, stir at room temperature for 24 hours, centrifuge for 15 minutes at a speed of 10,000 rpm, disperse the sediment at the bottom of the centrifuge tube with ultrapure water, centrifuge again, and repeat 3 times to obtain the described Perfluorohexane-polydopamine nanoparticles;

[0044] ...

Embodiment 3

[0047] The particle size characterization of embodiment 3 polydopamine perfluorohexane nanoliposomes

[0048] Detect the particle diameter of the polydopamine perfluorohexane nanoliposome (concentration is 1.55mg / mL) prepared by dynamic light scattering (DLS) in embodiment 1, get 1mL diluent and detect with Malvern potential particle size analyzer, gained particle diameter The result is as image 3 As shown, the size of the nanoparticles is about 200 nm.

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Abstract

The invention provides polydopamine perfluorohexane nanoliposome and a preparation method and application thereof, and relates to nano materials and biomedical materials. The preparation method comprises the steps that 1), perfluorohexane is subjected to ultrasonic emulsification and dispersion in ultrapure water to form uniform nano emulsion; 2), the nano emulsion is blended with ammonium hydroxide and an ethanol solution, a dopamine hydrochloride solution is added, and stirring, centrifuging and cleaning are conducted; 3), ferrous lactate is added into a perfluorohexane-polydopamine nanoparticle solution, and stirring, centrifuging and cleaning are conducted; 4), soybean lecithin, cholesterol and distearoyl phosphatidyl ethanolamine are dissolved in dichloromethane, a liposome film is synthesized through a rotary evaporator, and a liposome film solution is subjected to ultrasonic dispersion; and 5), the liposome film solution is added into the nanoparticle solution in the step 3), ultrasonic dispersion and self-assembly are conducted, centrifuging and cleaning are conducted, and the polydopamine perfluorohexane nanoliposome is obtained. The defect that perfluorohexane is insoluble in water is overcome, the size of nanoparticles is small, the nanoparticles can be passively targeted to tumor cells through the high-permeability long-retention effect, tumor microenvironment hypoxia is improved, and iron death of the tumor cells is triggered.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedical materials, and specifically relates to the ability of nanoliposomes to reach tumor sites, release oxygen to relieve tumor hypoxia, and release ferrous ions to drive Fenton reaction to trigger tumor cell ferroptosis, thereby achieving A polydopamine-perfluorohexane nanoliposome for the purpose of suppressing tumors and its preparation method and application. Background technique [0002] Cancer is one of the serious threats to human health. Rapid growth of tumor cells and twisted blood vessels lead to insufficient oxygen supply. Insufficient oxygenation in the tumor microenvironment (TME) leads to a hypoxic state, which not only promotes rapid tumor proliferation, angiogenesis, and metastasis, but is also a cause of treatment failure due to drug resistance in radiotherapy, chemotherapy, and immunotherapy . Therefore, improving hypoxia in tumor sites plays a vital role in tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/02A61K33/26A61K47/24A61K47/28A61K47/34A61P35/00
CPCA61K9/1277A61K31/02A61K33/26A61K47/34A61K47/28A61K47/24A61P35/00A61K2300/00
Inventor 石巍雷祥孙亚楠葛东涛
Owner XIAMEN UNIV
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