Preparation method of high-purity garlic polysaccharide

A garlic polysaccharide, high-purity technology, which is applied in the directions of medical preparations, pharmaceutical formulations, and digestive systems containing active ingredients, can solve the problems that the product purity is not high, it is difficult to realize industrialization, and it is not suitable for industrial production.

Pending Publication Date: 2021-09-14
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patented garlic polysaccharide has low purity and is not suitable for industrial production
And the integrated extraction process is used to extract garlic polysaccharides, garlic oligopeptides and odorless garlic powder from garlic residue. Although the process is simple, it is difficult to realize industrialization, and the obtained products are only crude products, which need further processing and refining
[0004] CN107177579A discloses a method for preparing alliinase, allicin and garlic polysaccharide by using garlic chip processing wastewater, adopting ultrafiltration membrane to concentrate and collect alliin crude enzyme liquid, CH 2 Cl 2 Extract the permeate to obtain allicin, CH 2 Cl 2 The raffinate is spray-dried to obtain the garlic polysaccharide extract. Although the process is simple, the purity of the obtained garlic polysaccharide is not high, and it cannot be directly applied in industrial production
[0005] CN108840957A discloses a process for extracting and purifying garlic polysaccharides. Garlic polysaccharides are extracted by means of degreasing, ultrasonic treatment, enzyme treatment, cooking, alcohol precipitation, separation, and drying. The process is relatively complicated, and the use of ultrasonic extraction and ethanol solvent increases Difficulty in cost and large-scale production, there is no process for removing protein, and the product purity cannot reach a high level

Method used

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  • Preparation method of high-purity garlic polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 500g of garlic slice water produced by processing dehydrated garlic slices, in which the garlic polysaccharide content is 6.4%, heat up to 30°C, add milk of lime to adjust the pH value to 9.0, keep warm for 10min, then heat up to 70°C, add lime with 1% of the weight of the feed liquid milk, keep warm for 20min; 2 Adjust the pH value to 9.0, heat up to 80°C after filtration, and pass CO 2 Adjust the pH value to 7.0, and filter again to obtain the clear liquid. Add 1% activated carbon to the clear liquid, raise the temperature to 30°C and stir for 30 minutes, filter and apply cation exchange resin LX-001 and anion exchange resin D296, then concentrate the column liquid and spray dry to obtain garlic polysaccharide. The garlic polysaccharide has a purity of 98.3%, a whiteness of 94%, no allicin detected, and no peculiar smell or taste of garlic. 1L ion exchange resin regeneration can process 9.3kg garlic polysaccharide product once.

Embodiment 2

[0033] After the garlic is crushed, extract it with water, take 500g of the extract, in which the garlic polysaccharide content is 7.5%, raise the temperature to 40°C, add lime milk to adjust the pH value to 10.0, heat it up to 80°C after holding for 30 minutes, add lime milk with 2% weight of the feed liquid , keep warm for 30min; pass CO 2 Adjust the pH value to 10.0, heat up to 90°C after filtration, and pass CO 2 Adjust the pH value to 7.5, and filter again to obtain the clear liquid. Add 1% activated carbon to the clear liquid, raise the temperature to 30°C and stir for 30 minutes, filter and apply cation exchange resin LX-001 and anion exchange resin D296, then concentrate the column liquid and spray dry to obtain garlic polysaccharide. The garlic polysaccharide has a purity of 98.6%, a whiteness of 93%, no allicin detected, and no peculiar smell or taste of garlic. 1L ion exchange resin regeneration can process 9.1kg garlic polysaccharide product once.

Embodiment 3

[0035] After the garlic is crushed, extract it with water, take 500g of the extract, in which the garlic polysaccharide content is 7.3%, raise the temperature to 35°C, add milk of lime to adjust the pH value to 9.5, heat it up to 85°C after holding for 30 minutes, add milk of lime with a weight of 2% of the feed liquid , keep warm for 30min; pass CO 2 Adjust the pH value to 10.0, heat up to 90°C after filtration, and pass CO 2 Adjust the pH value to 7.5, and filter again to obtain the clear liquid. Add 1% activated carbon to the clear liquid, raise the temperature to 30°C and stir for 30 minutes, filter the upper cation exchange resin LX-001 and anion exchange resin D296, and lower the column liquid for continuous chromatographic separation, using calcium-type strong cation resin as filler, optional Garlic polysaccharides with a degree of polymerization > 10 and garlic oligosaccharides with a degree of polymerization ≤ 10 are collected separately, concentrated, and spray-drie...

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Abstract

The invention provides a preparation method of high-purity garlic polysaccharide. The preparation method comprises the following steps: (1) cleaning: performing a cleaning process on a garlic extracting solution to obtain a clear solution A; (2) activated carbon adsorption: adding activated carbon into the clear liquid A, further adsorbing and deodorizing, and filtering to obtain clear liquid B; (3) desalting and decoloring: performing cation and anion exchange resin treatment on the clear liquid B to obtain a desalted and decolored liquid; and (4) concentrating and drying: concentrating and drying the desalted and decolored solution to obtain a garlic polysaccharide product with the purity of higher than 98%. According to the preparation method, the raw materials are easy to obtain, the technological process is simplified, the yield is high, full utilization of garlic resources is achieved, and the production cost of garlic polysaccharide is reduced.

Description

technical field [0001] The invention relates to the separation and purification of natural products, in particular to an extraction and refining process of garlic polysaccharide. Background technique [0002] In recent years, a large number of studies at home and abroad have confirmed that garlic polysaccharide has a variety of physiological activities, and has a good development prospect in health food and medicine. Garlic polysaccharide is an active ingredient in garlic, which has a special health function, and is a good material for the production of health food, with high development value. In addition to polysaccharides, garlic extract also contains solids, allicin, pigments, reducing sugars, organic acids, proteins, pectin, amides, flavonoids, fats, ammonium salts, sodium potassium calcium magnesium metal ions and other impurities. In addition, it also contains garlic's unique pungent taste substances. How to remove these impurities and odors needs to be solved urgent...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P1/14
CPCC08B37/0003A61K31/715A61P1/14
Inventor 高伟卢庆国梁星的曹通骆霜霜
Owner CHENGUANG BIOTECH GRP CO LTD
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