Red-knees herb extract and quality detection method of red-knees herb

A quality detection method and extract technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as multiple impurity peaks, uneven baseline of fingerprints, and influence on quality control of Polygonum papaya, to achieve accurate quality detection and improved resolution Effect

Pending Publication Date: 2021-09-21
四川辅正药业股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Yet in the above-mentioned detection method to the HPLC fingerprint chromatogram of Polygonum capsicum, the method for the pretreatment of Polygonum capsicum and chromatographic conditions etc. are still not perfect enough, make in the fingerprint collection of obtaining uneven baseline, there are many impurity peaks, isolated capsicum The number of active ingredients in Polygonum is small, and these problems seriously affect the quality control of Polygonum

Method used

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  • Red-knees herb extract and quality detection method of red-knees herb
  • Red-knees herb extract and quality detection method of red-knees herb
  • Red-knees herb extract and quality detection method of red-knees herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The high-performance liquid chromatography detection of embodiment 1 Polygonum polygonum

[0066] 1. Preparation of the test solution

[0067] Accurately weigh 1g of Polygonum pimentosa sample, put it in a 50mL Erlenmeyer flask, add 30mL of 60% ethanol precisely, soak for 2h, ultrasonically treat at 80°C for 50min, filter, evaporate to dryness in a water bath at 80°C, dissolve with methanol and dilute to 1ml, add 0.22 μm microporous membrane filtration, take the continued filtrate, that is.

[0068] 2. Preparation of reference solution

[0069] Accurately weigh each reference substance respectively, add methanol in an appropriate amount, and prepare a mixture containing eugenol (75μ / ml), catechin (0.05mg / ml), rutin (0.5mg / ml), proanthocyanidin B2 (1.5mg / ml) , kaempferol (0.5mg / ml), hyperin (0.25mg / ml), quercetin (0.25mg / ml), isorhamnetin (2.5mg / ml) mixed reference solution.

[0070] 3. Carry out HPLC detection on the test solution and the reference solution

[0071]...

experiment example 1

[0078] Experimental Example 1 Methodological Verification

[0079] 1. Selection of mobile phase system

[0080] Use 0.1% formic acid aqueous solution-acetonitrile, 0.3% triethylamine phosphate-acetonitrile, etc. as the mobile phase system for isocratic or different gradient elution, and finally determine the gradient elution with 0.1% formic acid aqueous solution (A)-acetonitrile (B). : 5~15min, 0%~15% mobile phase B; 15~25min, 25%~40% mobile phase B; 40~50min, 30%~5% mobile phase B.

[0081] 2. Selection of detection wavelength

[0082] 260nm, 230nm and 190nm were used as detection wavelengths for investigation respectively, and finally 260nm with more peaks, large peak area and good resolution was selected as detection wavelength.

[0083] 3. Detection method

[0084] According to the method under "Example 1", the solution of Polygonum papaya for testing was prepared, and the fluidity system obtained under "Experimental Example 1" was used for gradient elution. Flow rate...

experiment example 2

[0092] Experimental Example 2 Establishment of Fingerprint Spectrum of Polygonum Capsicum and Identification of Common Peaks

[0093] This experimental example utilizes the chromatograms (such as figure 1 shown) to establish the control fingerprint of Polygonum sp. and identify the common peaks.

[0094] 1. Establishment of fingerprints of Polygonum capsicum

[0095] The obtained raw data were uniformly integrated by Chromeleon 7 software, and then analyzed by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints (version 2012). Select the spectrum of sample S2 (C3) as the reference spectrum, use the median method, and set the time window width to 0.1. After multi-point calibration and Mark peak matching, the control fingerprint of Polygonum spicosa is generated. Control fingerprints such as image 3 shown.

[0096] 2. Similarity Evaluation of Polygonum Capsicum Fingerprints

[0097] With reference to the reference fingerprint of Po...

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Abstract

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and particularly relates to a red-knees herb extract and a quality detection method of red-knees herb. The red-knees herb extract provided by the invention is detected by adopting high performance liquid chromatography and has at least eight characteristic peaks, and the relative retention time is as follows: 0.39-0.59 of catechin; 0.45 to 0.67 of procyanidine B2; 0.57 to 0.85 of eugenol; 0.65 to 0.98 of rutin; 0.67 to 1.01 of hyperoside; 1 of quercitrin; 1.02 to 1.52 of isorhamnetin; and 1.07 to 1.61 of kaempferol. The red-knees herb quality detection method comprises the following steps: (1) preparing a test solution; and (2) detecting the high performance liquid chromatogram of the test solution. The extraction conditions and the chromatographic conditions of the red-knees herb are optimized, so that the quality of the red-knees herb can be detected more conveniently and accurately. The method has a good application prospect in quality control of the red-knees herb.

Description

technical field [0001] The invention belongs to the technical field of quality detection of traditional Chinese medicines, and in particular relates to a quality detection method of Polygonum capericum extract and Polygonum capsicum. Background technique [0002] Spicy Polygonum is the whole plant of Polygonum hydropiper L. in the family Polygonaceae. Distributed in various provinces in the north and south of my country. Have expelling wind and promoting diuresis, dissipating blood stasis and relieving pain, detoxification and detumescence, the effect of killing parasites and relieving itching. It is commonly used in the treatment of dysentery, gastroenteritis, diarrhea, rheumatic arthralgia, swelling and pain from falls, dysfunctional uterine bleeding and other diseases; it is externally used for snake bites and skin eczema. [0003] Due to differences in origin, species, harvest time, etc., the quality of Polygonum papaya medicinal materials varies greatly, and the curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 孙政姚仁川李兵黄勤挽张晓瑞李晓秋
Owner 四川辅正药业股份有限公司
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