Application of SAP as diagnostic marker for periodontitis

Abstract

The invention relates to the field of biomedical treatment, in particular to application of SAP as a diagnostic marker for periodontitis. Inventors of the invention accidentally discover that the SAP plays an important role in occurrence and development of the periodontitis. The inventors deeply research the action and molecular mechanism of the SAP on the periodontitis by using SAP-gene-modified mice, flow cytometry and transcriptome sequencing, and then further discover that the SAP expression of local gingivae of a severe periodontitis patient is increased; after a periodontitis model of an SAP-knocked-out mouse is established, by regulating and controlling M1-type polarization of gingival local macrophages, the activity of osteoclasts is enhanced, the expression of inflammatory factors IL-1beta, IL-6 and TNF-alpha is increased, and the development of the periodontitis is obviously aggravated; and a sequencing result shows that after the SAP is knocked out, the expression of inhibiting factors Sost and Dkk1 of a Wnt/beta-catenin signal channel is up-regulated, and the expression is possibly related to interaction between the macrophages and the osteoblasts.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of SAP as a periodontitis diagnostic marker. Background technique [0002] Periodontitis is a chronic immune inflammatory reaction against the periodontal supporting tissues (alveolar bone, periodontal ligament, gingiva and cementum) under the joint action of local stimulating factors and systemic factors. Pocket formation, alveolar bone resorption, tooth loosening and displacement, eventually lead to tooth loss, seriously affecting the patient's aesthetics, chewing, pronunciation and other physiological functions, and reducing the quality of life of the patient. Periodontitis not only causes tooth loss, but also is closely related to systemic diseases such as atherosclerosis, rheumatoid arthritis and diabetes. It is a major public health problem that needs to be solved urgently. At present, the clinical treatment of periodontitis is mainly symptomatic treatment for lo...

AI Technical Summary

Problems solved by technology

These examination methods are highly subjective, and can only diagnose patients with periodontitis, but cannot carry out early diagnosis and early intervention for potential patients with periodontitis

Especially for patients with aggressive periodontitis, it is difficult to be diagnosed by clinical examination in the early stage. When aggressive periodontitis is clinically diagnosed, it is usually in the late stage of periodontitis, and the affected teeth cannot be preserved, thus affecting the appearance, pronunciation and chewing of patients. function and quality of life

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