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Protein-free and DMSO-free cell freezing medium as well as application and preparation method thereof

A cryopreservation, protein-free technology, applied in the field of cell culture, can solve the problems of unclear composition of cryopreservation solution and cumbersome programmed freezing operation, and achieve the effects of clear composition, long shelf life and easy use.

Active Publication Date: 2021-10-22
苏州依科赛生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a protein-free, DMSO-free cell cryopreservation solution, its application and its preparation method, aiming to solve the problem that the composition of the cryopreservation solution in the prior art is not clear, the addition of DMSO components has cytotoxicity, and the technology of cumbersome programmed freezing operation is required defect

Method used

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  • Protein-free and DMSO-free cell freezing medium as well as application and preparation method thereof
  • Protein-free and DMSO-free cell freezing medium as well as application and preparation method thereof
  • Protein-free and DMSO-free cell freezing medium as well as application and preparation method thereof

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Embodiment 1

[0035] A protein-free, DMSO-free cell freezing medium:

[0036] Including the following components: various amino acids, various vitamins, various inorganic salts, sucrose, propylene glycol, sodium carboxymethylcellulose.

[0037] Preferably, the components of the cell cryopreservation solution are: calcium chloride dihydrate 0.2g / L, potassium chloride 0.2g / L, sodium chloride 6.8g / L, anhydrous disodium hydrogen phosphate 0.1g / L , Sodium dihydrogen phosphate monohydrate 0.0543g / L, zinc sulfate heptahydrate 0.000432g / L, sodium bicarbonate 1.8g / L, D-glucose 2g / L, pyruvate 0.06g / L, DL-lipoic acid 0.00015g / L L, 1,4-butanediamine dihydrochloride 0.000081g / L, linoleic acid 0.000042g / L, L-alanine 0.034g / L, L-alanine-L-glutamine 0.54253g / L , L-arginine hydrochloride 0.1475g / L, L-asparagine 0.04g / L, L-aspartic acid 0.13g / L, L-asparagine methyl ester hydrochloride 0.075g / L, L-cystine hydrochloride 0.025g / L, L-cysteine ​​hydrochloride monohydrate 0.02g / L, L-glutamic acid 0.0147g / L, glyc...

Embodiment 2

[0055] A protein-free, DMSO-free cell freezing solution:

[0056] Including the following components: various amino acids, various vitamins, various inorganic salts, sucrose, propylene glycol, sodium carboxymethylcellulose.

[0057] Preferably, the components of the cell cryopreservation solution are: calcium chloride dihydrate 0.2g / L, potassium chloride 0.2g / L, sodium chloride 6.8g / L, anhydrous disodium hydrogen phosphate 0.1g / L , Sodium dihydrogen phosphate monohydrate 0.0543g / L, zinc sulfate heptahydrate 0.000432g / L, sodium bicarbonate 1.8g / L, D-glucose 2g / L, pyruvate 0.06g / L, DL-lipoic acid 0.00015g / L L, 1,4-butanediamine dihydrochloride 0.000081g / L, linoleic acid 0.000042g / L, L-alanine 0.034g / L, L-alanine-L-glutamine 0.54253g / L , L-arginine hydrochloride 0.1475g / L, L-asparagine 0.04g / L, L-aspartic acid 0.13g / L, L-asparagine methyl ester hydrochloride 0.075g / L, L-cystine hydrochloride 0.025g / L, L-cysteine ​​hydrochloride monohydrate 0.02g / L, L-glutamic acid 0.0147g / L, gl...

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Abstract

The invention relates to a protein-free and DMSO-free cell freezing medium as well as application and a preparation method thereof. The cell freezing medium comprises various amino acids, various vitamins, various inorganic salts, saccharides, polyhydric alcohols and organic macromolecular compounds. The cell freezing medium is simple and clear in component and low in cost, the high-safety macromolecular compounds, the saccharides and the polyhydric alcohols are combined with other components, efficient preservation of cells in low-temperature and ultralow-temperature states is protected, the components are clear, stable and free of protein components, immunoreaction possibly caused in clinical application can be avoided, safety is improved, and the product is generally suitable for freezing storage of various types of cells. The invention also provides an application method of the cell freezing medium, the cells preserved by the cell freezing medium are recovered after one month of short-term cryopreservation, the cell viability can be maintained at 95% or more, the cells are recovered after one year of preservation, the cell viability can still reach 90% or more, and the cell viability and function are not affected.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a protein-free and DMSO-free cell cryopreservation solution and its application. Background technique [0002] With the development of medical technology and the advancement of cell therapy methods, the requirements for in vitro cell culture are also getting higher and higher. The cell culture medium and cryopreservation solution in the prior art usually contain animal-derived substances with complex components. There are potential risks and increased chances of contamination. Therefore, the introduction of serum-free and protein-free cell culture media and cryopreservation solutions is extremely important for the research and development of medical research, and also provides the necessary technical basis for subsequent gene editing and individualized cell therapy. [0003] Cell cryopreservation, that is, by adding cryoprotectants to the solution, the cells are...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 于宝利陈旭陈刚
Owner 苏州依科赛生物科技股份有限公司
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