Propolis alcohol extract-cyclodextrin-chitosan-mucoprotein sustained-release gel as well as preparation method and application thereof

A technology of slow-release gel and chitosan, which is applied in the direction of drug combination, pharmaceutical formula, and medical preparations of non-active ingredients, etc. It can solve the problems of large influence of cyclodextrin ratio, difficult control of encapsulation rate, and impact on application , to achieve the effect of increasing the coating rate, promoting ulcer healing, and helping value-added growth

Inactive Publication Date: 2021-10-22
梓森科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, propolis is soluble in ethanol and has poor solubility in water, which affects its application in hydrogel formulations
Therefore, it is a common method to use cyclodextrin to embed propolis ethanol extract in clinical use, but the encapsulation rate of this method is difficult to control, and is greatly affected by the ratio of cyclodextrin

Method used

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  • Propolis alcohol extract-cyclodextrin-chitosan-mucoprotein sustained-release gel as well as preparation method and application thereof
  • Propolis alcohol extract-cyclodextrin-chitosan-mucoprotein sustained-release gel as well as preparation method and application thereof
  • Propolis alcohol extract-cyclodextrin-chitosan-mucoprotein sustained-release gel as well as preparation method and application thereof

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preparation example Construction

[0028] The invention provides a preparation method of propolis alcohol extract-cyclodextrin-chitosan-mucin slow-release gel, comprising the following steps:

[0029] (1) performing acid treatment, alkali treatment and chitosan enzymatic hydrolysis on mussel shells in sequence to obtain mussel chitosan enzymatic hydrolysis products;

[0030] Mixing the mussel chitosan enzymatic hydrolysis product with citric acid, sodium dihydrogen phosphate, and water, and performing a first reaction to obtain a chitosan-citric acid complex;

[0031] (2) Mix the chitosan-citric acid complex, β-cyclodextrin and buffer solution, and carry out the second reaction to obtain the cyclodextrin-chitosan complex;

[0032] (3) the cyclodextrin-chitosan complex is mixed with propolis alcohol extract and water ultrasonically, and the propolis alcohol extract wrapped in cyclodextrin-chitosan is obtained after solid-liquid separation;

[0033] (4) mixing sodium carboxymethyl cellulose, xanthan gum and wate...

Embodiment 1

[0064] (1) Mechanically pulverize mussel shells and pass through a 100-mesh sieve, then dilute and digest the powder with 2wt% nitric acid solution at a ratio of 1:10 by weight to volume, and digest for 24 hours to remove inorganic matter in the shells, wash with pure water and wash with 2M sodium hydroxide The solution was treated for 3 hours according to the ratio of weight to volume 1:10, washed and diluted with 1wt% glacial acetic acid to make a 5% (w / v) mussel chitosan solution at 10U g -1 Add the chitosan enzyme solution in the ratio of 40 ° C for 5 hours, inactivate in a boiling water bath for 30 minutes. Sodium hydrogen was added to 250mL of purified water in a weight ratio of 1:2:1, and stirred to dissolve. React at 85°C for 4 hours, precipitate with absolute ethanol, and freeze-dry to obtain a chitosan-citric acid complex;

[0065] (2) Prepare 7.5g of β-cyclodextrin and 1.5g of chitosan-citric acid complex obtained in step (1), stir and dissolve in 500mL of 0.1M sod...

Embodiment 2

[0068] The difference between embodiment 2 and embodiment 1 is that the add-on of cyclodextrin-chitosan complex is 4g in the step (3), and all the other operations are the same, obtain the propolis alcohol extract of cyclodextrin-chitosan wrapping, Denoted as sample 2.

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Abstract

The invention provides propolis alcohol extract-cyclodextrin-chitosan-mucoprotein sustained-release gel as well as a preparation method and an application thereof, and belongs to the technical field of sustained-release medicines. According to the invention, a sodium carboxymethyl cellulose-mussel mucoprotein polymer and a propolis alcohol extract wrapped by cyclodextrin-chitosan are subjected to cross-linking compounding, and a catechol structure of mussel mucoprotein can be oxidized into an o-quinone structure under an alkaline or oxidation condition of a mussel mucoprotein compound and mussel shell glycan; a stable sustained-release structure is formed by combining the active amino group on the surface of mussel shell glycan through a reaction similar to Schiff base, so that on one hand, the gel is enabled to be attached to an ulcer surface to form a long-term protection and administration system while being quickly gelled; on the other hand, the unique porous elastic structure of the mussel shell glycan realizes gradual slow release of the propolis alcohol extract, the propolis alcohol extract continuously acts on the ulcer, and the clinical effect is better.

Description

technical field [0001] The invention relates to the technical field of sustained-release medicines, in particular to a propolis alcohol extract-cyclodextrin-chitosan-mucin sustained-release gel and its preparation method and application. Background technique [0002] Propolis is a viscous substance formed by mixing resin and other secretions of glue source plants collected by worker bees with secretions such as mandibular glands and wax glands. It has a broad-spectrum antibacterial effect and can inhibit Staphylococcus aureus, Streptococcus and other bacteria , which has a special inhibitory effect on oral pathogenic bacteria. Because propolis has a wide range of biological activities, its antibacterial, anti-inflammatory, pain-relieving, healing-promoting and immune-enhancing effects are clinically used to treat oral ulcers. [0003] However, propolis is soluble in ethanol and has poor solubility in water, which affects its application in hydrogel formulations. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K35/644A61K9/06A61K47/42A61P1/02
CPCA61K47/6951A61K47/6949A61K35/644A61K9/06A61K47/42A61P1/02
Inventor 张铭唐星龙姚阿明
Owner 梓森科技股份有限公司
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