Micro-fluidic chip based on electrohydrodynamics and micro sample application device and method
A microfluidic chip, kinetic technology, applied in chemical instruments and methods, measuring tubes/pipettes, laboratory containers, etc. Safe and easy to operate, easy to operate and safe, accurate effect of fixing effect
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Embodiment 1
[0067] Preparation of microfluidic chip:
[0068] The prepared microfluidic chip structure is as follows figure 1 As shown, the chip is an integrated structure.
[0069] ①Design with CAD such as figure 1 The channel structure of the microfluidic chip shown, including microchannels 1-7, capillary embedded channels at the outlet of the microchannels, optical fiber channels 1-3, and electrode channels, is printed with a film mask. Drop the SU-8 photoresist on the cleaned silicon wafer to shake the glue, the thickness is about 120um. Finally, it is covered with a mask, exposed under an exposure machine, and the uncured part is cleaned with a developing solution to obtain a silicon wafer template.
[0070] ②Mix PDMS monomer and curing agent in a certain proportion to obtain PDMS polymer. Pour the PDMS polymer on the silicon wafer template with a thickness of about 1-10 mm, and obtain a PDMS chip with a channel structure after drying.
[0071] ③ On the PDMS chip with a channel ...
Embodiment 2
[0077] A microbial spotting device based on electrohydrodynamics, such as Figure 4 As shown, it includes: a microfluidic chip 1, the microfluidic chip has at least two sample inlets, and is provided with two opposite optical fiber channels;
[0078] Laser transmitter 2, which emits visible light band laser;
[0079] A laser detector 3 is used to detect an optical signal and convert the optical signal into an electrical signal; the laser detector includes but is not limited to a photodiode or a photomultiplier tube;
[0080] Two optical fibers, the optical fibers are embedded in the fiber channel, one of which is connected to the laser transmitter, and the other is connected to the laser detector;
[0081] The relay 5 is used to control the switch of the circuit;
[0082] A high-voltage DC power supply 6, the two poles of the high-voltage DC power supply are respectively connected to the upper electrode and the lower electrode of the microfluidic chip;
[0083] The data acq...
Embodiment 3
[0087] Taking the microfluidic chip applied to the coupling interface of flow sorting and mass spectrometry after microbiome droplet culture as an example, the chip structure is based on the chip structure prepared in Example 1 ( figure 1 ) as an example:
[0088] 1. Liquid culture and amplification of microorganisms in droplets
[0089] Use the common microfluidic cross-shaped droplet generation chip to generate droplets from the selection medium containing microorganisms. The droplet size is 10um-100um, preferably 80um. Microorganisms proliferate and fill the droplets.
[0090] 2. Label-free growth phenotype droplet sorting based on scattered light
[0091] Cultured droplets can be sorted using a label-free outgrowth phenotype droplet sorting system based on scattered light. Droplets overgrown with target microorganisms can be sorted and collected into one-milliliter centrifuge tubes.
[0092] 3. Separation of individual droplets
[0093] The microfluidic chip printed by...
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