Preparation and application method of biological purifying agent for removing composite pollutants in sediments
A biological purifier and compound pollution technology, applied in biochemical equipment and methods, microbial-based methods, water pollutants, etc. It is easy to realize industrialization, good arsenic fixation effect and high adsorption performance.
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[0029] The present invention will be described in further detail below in combination with specific embodiments of the present invention.
[0030] 1. At first, the preparation of Penicillium chrysogenum suspension comprises the following steps:
[0031] (1) Potato medium: Peel potatoes, cut 200 g into small pieces, add 1000 mL of distilled water, boil for 25-35 minutes, pass through 6-7 layers of gauze, add 20.0 g of glucose to the filtrate and make up water to 1000 mL. Prepare ten 500 mL Erlenmeyer flasks, add 20.0 mL of potato culture solution and 180.0 mL of distilled water respectively, seal them with 12 layers of gauze, put them in a steam sterilizer, and seal the upper port of the Erlenmeyer flasks with kraft paper. Sterilization conditions: pressure 0.13 MPa, temperature about 120°C, time 25 min, after the sterilization is completed, take it out after cooling to room temperature, and set aside.
[0032] (2) Penicillium chrysogenum suspension: transfer the Penicillium ...
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