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Ralstonia solanacearum extraction composition, extract and method for identifying ralstonia solanacearum in casuarina equisetifolia

A technology for bacterial wilt and casuarinas, which is applied in the field of forest disease detection and control, can solve the problems of reducing the selection efficiency of disease-resistant plants, cumbersome and complicated processes, and high damage rate of forest trees, and achieves protection of disease-resistant plants, high detection efficiency, and improved preservation. rate effect

Pending Publication Date: 2021-10-29
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the traditional identification method to identify whether casuarina is infected with R. solanacearum is a very cumbersome and complicated process, takes a long time, and has a high damage rate to forest trees, which reduces the selection efficiency of disease-resistant plants.

Method used

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  • Ralstonia solanacearum extraction composition, extract and method for identifying ralstonia solanacearum in casuarina equisetifolia
  • Ralstonia solanacearum extraction composition, extract and method for identifying ralstonia solanacearum in casuarina equisetifolia
  • Ralstonia solanacearum extraction composition, extract and method for identifying ralstonia solanacearum in casuarina equisetifolia

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preparation example Construction

[0047] In a third aspect, the present invention provides a method for preparing the R. solanacearum extract as described above, the preparation method comprising: mixing the water, the sodium chloride and the peptone.

[0048] In one example, the preparation method further includes the step of sterilizing the mixed product.

[0049] In the fourth aspect, the present invention provides a method for identifying R. solanacearum in Casuarina, the identification method includes the steps of extracting R. solanacearum from the plant tissue of Casuarina to be tested and identifying it. Bacteria extract.

[0050] The present invention does not specifically limit the way of extraction, including but not limited to the way of leaching, the leaching time is 15min-25min, for example including but not limited to the following time lengths or the range between these time lengths: 15min, 16min, 17min, 18min, 19min, 20min. The extraction can be carried out under field conditions, and the co...

Embodiment 1

[0056] The present embodiment provides a kind of method for fast identification of casuarina equisetifolia (Casuarina equisetifolia) bacterial wilt in the field, comprising the following steps:

[0057] (1) Configure Ralstonia solanacearum extract

[0058] Accurately weigh 8g NaCl and 10g peptone in the laboratory, first add 50mL distilled water to fully dissolve, and quantify to 1000mL. Use an autoclave to sterilize at 120°C for 30 minutes, and set aside.

[0059] (2) Selection of infected plants and healthy plants

[0060] In the bacterial wilt disease-affected area, the plants with most of the withered and yellowed branches (denoted as "treatment 1") and the completely healthy growth plants (denoted as "treatment 2") in the branches were selected. This forest area has been afforested for 4 years. The average height is 7m, and the average DBH is 6.21cm.

[0061] In addition, in the experimental forest far away from bacterial wilt disease-affected areas, plants with wither...

Embodiment 2

[0079] The present embodiment provides a kind of method for quick identification of Casuarina equisetifolia bacterial wilt in the field, except that the R. solanacearum extract is set at 5 sodium chloride concentrations (0.7%, 0.75%, 0.8%) in step (1). , 0.85% and 0.9%), and in step (2), only select 3 plants with withered branches (embodiment 1 "treatment 1"), each plant was repeatedly measured 3 times, a total of 3 plants × 5 concentrations × 3 times Repeat = 45 times of detection, other operations are the same as in Example 1.

[0080] Using the conditions of Example 1, that is, with reference to steps (3) and (4), the infected plants were tested using different ratios of extracts.

[0081] The result shows that sodium chloride concentration is 0.9%, 0.85% and 0.7% Ralstonia solanacearum extract processing material does not detect Ralstonia solanacearum ( image 3 ), it is speculated that the possible reason is that too high or too low sodium chloride concentration is not c...

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Abstract

The invention provides a ralstonia solanacearum extraction composition, an extract and a method for identifying ralstonia solanacearum in casuarina equisetifolia. The composition comprises 7.5-8.3 parts of sodium chloride and 8-12 parts of peptone. Compared with the prior art, the ralstonia solanacearum extraction composition has the advantages that sodium chloride and agar are matched and combined in a proper proportion, the extract containing the composition can be used for quickly extracting ralstonia solanacearum from a small amount of casuarina equisetifolia plant tissues, the extracted ralstonia solanacearum is enough for identification, particularly for ralstonia solanacearum test paper identification, and a reliable means is provided for field on-site quick detection of ralstonia solanacearum.

Description

technical field [0001] The invention belongs to the field of detection and control of forest diseases, and in particular relates to an extraction composition of R. solanacearum, an extract and a method for identifying R. solanacearum in casuarina. Background technique [0002] Casuarina (Latin name: Casuarina) is wind-resistant and resistant to barrenness. It is widely used in the construction of tropical and subtropical coastal shelterbelts. It is the first barrier against natural disasters such as typhoons. Disaster reduction plays an extremely important role. [0003] Casuarina bacterial wilt is a devastating plant soil-borne disease caused by the pathogen Ralstonia solanacearum (also known as Ralstonia solanacearum). The disease spreads rapidly and has a high mortality rate. After being infected with R. solanacearum, casuarinas gradually show symptoms of wilting, drying up and even death. In the wild environment, affected by climatic conditions such as precipitation and...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/24C12R1/01
CPCC12Q1/04C12Q1/24G01N2333/195
Inventor 魏永成孟景祥张勇仲崇禄
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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