Microbial powder for preventing and treating strawberry root rot and preparation method thereof

A technology of strawberry root rot and microbial bacteria, which is applied in the field of agricultural microorganisms, can solve problems such as human ecological hazards, imbalance of soil microbial flora, physical damage of applicators, etc., and achieve the effect of increasing yield

Active Publication Date: 2021-11-02
微生物肥料技术研究推广中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the prevention and treatment of strawberry root rot is mainly to use drugs to fumigate the soil. Applying a large amount of drugs will not only cause some damage to the body of the applicator, but also lead to an imbalance of soil microbial flora. collection, will also cause harm to human ecology

Method used

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  • Microbial powder for preventing and treating strawberry root rot and preparation method thereof
  • Microbial powder for preventing and treating strawberry root rot and preparation method thereof
  • Microbial powder for preventing and treating strawberry root rot and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] 1. Screening and verification of pathogenic bacteria

[0037] Use a sterile kraft paper bag to collect the rhizomes of strawberry diseased plants with root rot, soak them in 70% alcohol for 30 seconds, transfer them to 0.1% mercuric chloride solution for surface disinfection for 5 minutes, then put them into sterile water and rinse them three times continuously to remove the residual disinfectant .

[0038] Move the sterilized diseased tissues to the plate culture medium, and put 4-6 pieces in each dish. Put the petri dish upside down and put it in an incubator at about 25°C for 3-6 days, then observe the growth results of the bacteria to be isolated. Pick single colonies that are relatively neat and consistent, inoculate them on the plate medium and separate and purify again until the pure strains are cultivated; store the pure strains of pathogenic bacteria in slant test tubes.

[0039] Through root irrigation, the pathogenicity of pathogenic bacteria to strawberrie...

Embodiment 2

[0080] In the strawberry planting demonstration base of the National Microbial Fertilizer Technology Research and Extension Center, where strawberries have been planted continuously for 5 years, eight treatments are set up, each treatment has three repetitions, and each plot is 110m 2 , strawberry is unified seedling cultivation, unified transplanting, unified management, uses respectively the microbial bacterial powder (processing 1: Bacillus subtilis+Paenibacillus colloid+Paecilomyces lilacinus) prepared by the embodiment of the present invention 1 with 20L / mu application rate +Trichoderma harzianum), Bacillus subtilis bacteria powder (processing 2), Paenibacillus colioids bacteria powder (processing 3), commercially available Bacillus subtilis (processing 4), commercially available Paenibacillus colioids bacteria powder (processing 5 ), Paecilomyces lilacinus powder (treatment 6), Trichoderma harzianum powder (treatment 7) and clear water (control). Field surveys were carri...

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Abstract

The invention discloses microbial powder for preventing and treating strawberry root rot and a preparation method thereof, and belongs to the field of agricultural microorganisms. The microbial bacterial powder for preventing and treating the strawberry root rot disease is prepared from the following components in parts by weight: 22 to 27 parts of bacillus subtilis bacterial powder, 23 to 28 parts of paenibacillus mucilaginosus bacterial powder, 21 to 26 parts of paecilomyces lilacinus bacterial powder and 22 to 28 parts of trichoderma harzianum bacterial powder. The bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.17701; the paenibacillus mucilaginosus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.17702. The strawberry root rot prevention and treatment effect is good, and the yield and quality of strawberries can be remarkably improved.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a microbial powder for preventing and treating strawberry root rot and a preparation method thereof. Background technique [0002] Strawberry is a perennial herb of the Rosaceae Fragaria genus. It has good color, fragrance and taste and high nutritional value. It is known as the "Queen of Fruits". [0003] Strawberry root rot is a common soil-borne disease that causes strawberry rhizome rot symptoms. According to relevant reports at home and abroad, the pathogenic fungi that cause strawberry root rot involve more than 20 species of multiple genera, including the genus Discochaete ( Pestalotiopsis sp.), Fusarium sp., Colletotrichum sp., Rhizoctonia sp., Phytophthora sp., etc. [0004] Strawberry root rot is a symptom of systemic damage to the whole strawberry plant. The main feature is that the pathogen infects the rhizome, some from the inside to the outside,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/38A01N63/30A01P3/00A01P21/00C12R1/125C12R1/07C12R1/79C12R1/885
CPCC12N1/20C12N1/14A01N63/22A01N63/38A01N63/30Y02W30/40
Inventor 王倩倩柳慧静马婷孟庆伟张少骅申术霞闫冲
Owner 微生物肥料技术研究推广中心
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