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Gradient density scaffold material as well as preparation method and application thereof

A technology of gradient density and scaffolding materials, applied in the field of additive manufacturing, can solve problems such as uneven stress distribution, difficulty in meeting comprehensive performance requirements, and difficulty in controlling microstructural interconnectivity

Active Publication Date: 2021-11-05
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional production methods of tissue engineering bone scaffolds mainly include electrospinning, salt immersion, mold compression and emulsification freezing / freeze drying, but most of them are prepared by using uniform and single synthetic composite materials, and the composition and concentration remain basically unchanged, which cannot meet the needs of the human body. The gradual change of bone structure and composition requires controllability. When used as a bone repair material, it does not fit well with natural bone and the stress distribution is uneven; and the interconnection of the internal microstructure of the scaffold is difficult to control, and it is difficult to restore the structure of natural bone tissue. Comprehensive performance It is difficult to meet the requirements and it is difficult to restore the natural bone function

Method used

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  • Gradient density scaffold material as well as preparation method and application thereof
  • Gradient density scaffold material as well as preparation method and application thereof
  • Gradient density scaffold material as well as preparation method and application thereof

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preparation example Construction

[0036] The invention provides a method for preparing a gradient density scaffold material, comprising the following steps:

[0037] Mix organic matter, inorganic matter and organic solvent to obtain liquid A; the organic matter includes one or more of polycaprolactone, polyvinyl alcohol, polylactic acid, gelatin, sodium alginate, collagen and fibrin; the Inorganic substances include one or more of hydroxyapatite, calcium phosphate, calcium tripolyphosphate, bioactive glass and calcium silicate;

[0038] Provide chitosan-acetic acid aqueous solution as B liquid;

[0039] dynamically mixing the liquid A and the liquid B, and performing additive printing on the obtained dynamic mixed liquid to obtain the gradient density scaffold;

[0040] The dynamic mixing is: extruding and mixing liquid A and liquid B according to different extrusion rates to obtain a dynamic mixed liquid.

[0041] In the present invention, unless otherwise specified, each component in the preparation method...

Embodiment 1

[0069] Mix and dissolve 8 g of polycaprolactone and 10 mL of dichloromethane in a closed container for 12 hours to obtain a polycaprolactone solution; mix the obtained polycaprolactone solution with 0.8 g of hydroxyapatite to obtain liquid A;

[0070] Mix 1 g of chitosan with 10 mL of acetic acid aqueous solution (the volume of acetic acid and deionized water is 1:9) to obtain liquid B;

[0071] Inject liquid A into the 3D bioprinting syringe of liquid A, inject liquid B into the 3D bioprinting syringe of liquid B, install the syringe and fix the piston, connect the air tanks through different air pressure controllers, and adjust the air pressure to 0.2MPa for ventilation Squeeze to remove the air in the end of the 3D bioprinting syringe, and let the air bubbles in the 3D bioprinting syringe settle;

[0072] Connect the needles of liquid A 3D bioprinting syringe and liquid B 3D bioprinting syringe to the two inlets of the U-shaped three-way valve respectively, the outlet of th...

Embodiment 2

[0081] Mix and dissolve 9 g of polycaprolactone and 10 mL of dichloromethane in a closed container for 12 hours to obtain a polycaprolactone solution; mix the obtained polycaprolactone solution with 1.5 g of hydroxyapatite to obtain liquid A;

[0082] Mix 0.5g chitosan with 10mL acetic acid aqueous solution (the volume of acetic acid and deionized water is 1:9) to obtain liquid B;

[0083] Inject liquid A into the 3D bioprinting syringe of liquid A, and inject liquid B into the 3D bioprinting syringe of liquid B. After installing the syringe and fixing the piston, connect the air tanks through different air pressure controllers, and adjust the air pressure to 0.3MPa for ventilation. Squeeze to remove the air in the end of the 3D bioprinting syringe, and let the air bubbles in the 3D bioprinting syringe settle;

[0084] Connect the needles of liquid A 3D bioprinting syringe and liquid B 3D bioprinting syringe to the two inlets of the U-shaped three-way valve respectively, the out...

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Abstract

The invention belongs to the technical field of biological manufacturing, and particularly relates to a gradient density scaffold material as well as a preparation method and application thereof. The preparation method of the gradient density scaffold material provided by the invention comprises the following steps: mixing an organic matter, an inorganic matter and an organic solvent to obtain a solution A; providing a chitosan-acetic acid aqueous solution as a solution B; dynamically mixing the solution A and the solution B, and carrying out additive printing on the obtained dynamic mixed solution to obtain the gradient density scaffold; and the dynamic mixing is as follows: extruding and mixing the solution A and the solution B according to different extrusion rates to obtain the dynamic mixed solution. According to the invention, the organic matter is beneficial to improving the elasticity and biological activity of the scaffold material; the inorganic matter and chitosan are beneficial to improving the hardness and the mechanical property of the scaffold material; and different scaffold composition solutions are respectively prepared , then the solution A and the solution B are dynamically mixed at different extrusion rates, and the additive printing is carried out to obtain the gradient density scaffold material.

Description

technical field [0001] The invention belongs to the technical field of additive manufacturing, and in particular relates to a gradient density support material and its preparation method and application. Background technique [0002] Natural bone is a nano-porous composite material with a gradient structure. The main components are hydroxyapatite and collagen. The organic matter gradually transitioned, that is, the main component of hydroxyapatite gradually decreased, and the collagen fibers gradually increased. At present, the solutions for bone defect repair include bone grafting or bone implantation, and the preparation of porous tissue engineering scaffolds using synthetic composite materials is an effective means to achieve bone defect repair. [0003] The traditional production methods of tissue engineering bone scaffolds mainly include electrospinning, salt immersion, mold compression and emulsification freezing / freeze drying, but most of them are prepared by using u...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/16A61L27/22A61L27/20A61L27/24A61L27/12A61L27/10A61L27/02A61L27/50A61L27/54B33Y70/10B33Y80/00
CPCA61L27/18A61L27/16A61L27/222A61L27/20A61L27/24A61L27/225A61L27/12A61L27/10A61L27/025A61L27/50A61L27/54B33Y70/10B33Y80/00A61L2430/02A61L2300/412C08L67/04C08L29/04C08L5/08C08L5/04Y02P10/25
Inventor 张海光王玉萍胡庆夕王国印
Owner SHANGHAI UNIV
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