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Solid cultivation method of nattokinase

A technology of nattokinase and cultivation method, applied in the field of microorganism cultivation, can solve the problems of easy inactivation, high temperature of fixed medium, low activity, etc., and achieves the effects of high operation efficiency, increased yield and activity, and increased specific surface area

Pending Publication Date: 2021-11-05
广西南宁佰奥吉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of nattokinase by traditional fermentation method is low, and nattokinase is unstable and easy to inactivate, thus greatly restricting the development and application of nattokinase products; in addition, the solid culture used in the production of nattokinase Due to insufficient contact between the culture medium and the strain, and the heat generated by the strain during fermentation is not easy to dissipate, the temperature of the fixed medium is high, resulting in low yield and low activity of nattokinase, which is difficult to meet the needs of use.

Method used

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  • Solid cultivation method of nattokinase

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A kind of solid cultivation method of nattokinase, inoculate the liquid strain of Bacillus subtilis natto on the culture medium, the inoculation amount of Bacillus subtilis natto is 8% of the weight of the culture medium; The culture medium comprises the following components: soybean peptone 1 %, beef extract 0.3%, sodium bicarbonate 0.4%, calcium chloride 0.03%, magnesium sulfate 0.01%.

[0030] The culture medium is adjusted to pH 7.0 with dilute acid solution, which is hydrochloric acid solution.

[0031] Before the Bacillus subtilis natto is inoculated in the culture medium, the yeast is inoculated on the culture medium, and the inoculum amount is 1%.

[0032] like Figure 1-4 As shown, the cultivation device includes an incubator 1, the side of the incubator 1 is provided with a door 11, and the bottom of the incubator 1 is provided with a discharge port 12; the incubator 1 is provided with a cultivation assembly 2, and the cultivation assembly 2 includes a tray 2...

Embodiment 2

[0043] A kind of solid culture method of nattokinase, inoculate the liquid strain of bacillus subtilis natto on the culture medium, the inoculation amount of bacillus subtilis natto is 10% of the weight of the culture medium; the culture medium comprises the following components: soybean peptone 1.5 %, beef extract 0.5%, sodium bicarbonate 0.6%, calcium chloride 0.04%, magnesium sulfate 0.01%.

[0044] The pH of the culture medium is adjusted to 7.2 with a dilute acid solution, and the dilute acid solution is a hydrochloric acid solution.

[0045] Before the Bacillus subtilis natto is inoculated in the culture medium, the yeast is inoculated on the culture medium, and the inoculation amount is 1.5%.

[0046] The cultivation apparatus used was the same as that of Example 1.

Embodiment 3

[0048] A kind of solid cultivation method of nattokinase, inoculate the liquid strain of bacillus subtilis natto on the culture medium, the inoculation amount of bacillus subtilis natto is 12% of the weight of the culture medium; the culture medium comprises the following components: soybean peptone 2 %, beef extract 0.8%, sodium bicarbonate 0.8%, calcium chloride 0.04%, magnesium sulfate 0.02%.

[0049] The culture medium is adjusted to pH 7.3 with dilute acid solution, which is hydrochloric acid solution.

[0050] Before the Bacillus subtilis natto is inoculated in the culture medium, the yeast is inoculated on the culture medium, and the inoculation amount is 2%.

[0051] The cultivation apparatus used was the same as that of Example 1.

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Abstract

The invention discloses a solid cultivation method of nattokinase. According to the method, a liquid strain of bacillus subtilis natto is inoculated onto a culture medium, wherein the inoculation amount of the bacillus subtilis natto is 8-12%; the culture medium comprises the following components: 1% of soy peptone, 0.5% of beef extract, 0.8% of sodium bicarbonate, 0.03-0.04% of calcium chloride and 0.02-0.04% of magnesium sulfate. According to the invention, the culture medium can be looser by virtue of gas generated during pH adjustment of sodium bicarbonate added into the culture medium and carbon dioxide generated by fermentation of saccharomycetes, so that the specific surface area of the culture medium is increased, the culture of bacillus subtilis natto is facilitated, and the yield and the activity of nattokinase are improved; in the cultivation process, the cultivation box can be divided into a plurality of different cultivation areas through partitions adopted by the cultivation device, and different use requirements in practice are met; a tray is connected with a rotating device, so that after strain cultivation is completed, a culture medium can be poured to the bottom of the cultivation box more rapidly through operation of the rotating device, and the solid cultivation method has the advantages of being convenient, rapid and high in operation efficiency.

Description

technical field [0001] The utility invention belongs to the technical field of microorganism cultivation, and in particular relates to a solid cultivation method of nattokinase. Background technique [0002] Nattokinase, an enzyme capable of dissolving blood clots, is an ideal potential drug for the treatment and prevention of blood clots. At present, the production of nattokinase by traditional fermentation method is low, and nattokinase is unstable and easy to inactivate, thus greatly restricting the development, popularization and application of nattokinase products; in addition, the solid culture used in the production of nattokinase Due to insufficient contact between the culture medium and the strains, and the heat generated by the strains during fermentation is not easy to dissipate, the temperature of the fixed medium is high, resulting in a low yield of nattokinase and low activity, which is difficult to meet the needs of use . Contents of the invention [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N9/56C12M1/20C12M1/10C12M1/14C12M1/00C12R1/125C12R1/645
CPCC12N1/20C12N1/16C12N9/54C12M21/16C12M21/14C12M23/34C12M23/04C12M27/02C12M27/10
Inventor 陈海玲黄新英黄时海高文功
Owner 广西南宁佰奥吉生物科技有限公司