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Preparation method and application of compounded Pu'er tea functional composition

A composition and technology of Pu'er tea, applied in the fields of biotechnology and medicine, can solve problems such as weak weight loss effect, achieve the effects of low side effects, improve non-alcoholic fatty liver, and improve symptoms of hyperlipidemia

Pending Publication Date: 2021-11-09
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the weight loss effect of using Pu'er tea alone is weak. For example, studies have shown that 3000mg of instant Pu'er tea can be administered to mice every day to show the effect of reducing the weight of mice.
At present, there is no report that compounding Pu’er tea extract, dendrobium polysaccharide extract and flavonoid extract from effective parts of Morus can improve high-fat and high-sugar diet-induced diseases

Method used

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  • Preparation method and application of compounded Pu'er tea functional composition
  • Preparation method and application of compounded Pu'er tea functional composition
  • Preparation method and application of compounded Pu'er tea functional composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: the preparation of Pu'er tea extract 1

[0069] Weigh 100g of dry Pu'er tea and mix with 400mL of distilled water, reflux at 70°C for 2h, filter, repeat three times, combine the filtrates, concentrate and dry to obtain Pu'er tea extract. According to formula (1)-(3) tea pigment determination method, the measured theabrownin content is 6.47%, which is higher than 0.27% of theaflavin and 1.91% of thearubigin, and is the main tea pigment in the Pu'er tea extract.

Embodiment 2

[0070] Embodiment 2: Preparation 1 of dendrobium polysaccharide

[0071] Mix 10 g of dried Dendrobium dendrobii with 200 mL of distilled water, stir at 500 rpm for 2 h, 400 W ultrasonic crushing for 20 min, adjust pH 7.0 with citric acid, add trichloroacetic acid to a final concentration (v / v) of 1.5% to precipitate protein, adjust pH 7 with NaOH , centrifuge at 4000r / min for 10min, collect the supernatant, concentrate in vacuo at 65°C to 0.2 times the original volume, add absolute ethanol to the concentrated solution to a final concentration of 80%, spend overnight at 4°C, centrifuge at 4000r / min for 10min, and take the precipitate, - Freeze-drying at 50°C to obtain the polysaccharide extract of Dendrobium dendrobii. According to the above polysaccharide determination method, the total polysaccharide content was measured to be 51%.

Embodiment 3

[0072] Embodiment 3: the preparation 1 of mulberry flavonoids

[0073]Weigh 50g of dried mulberry bark, crush it, add 500mL 80% ethanol aqueous solution, and extract at room temperature for 24h. The crude extract was suction-filtered twice with filter paper, the solvent was evaporated by a rotary evaporator, petroleum ether was added to remove grease, and the suspension was extracted three times by adding ethyl acetate-water solution with a volume ratio of 1:1, and the ethyl acetate phases were combined. After dehydration with sodium sulfate, the solvent was evaporated to obtain the flavone extract of Morizhi flavonoids. According to the above-mentioned total flavonoid determination method, the total flavonoid content is 34%.

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Abstract

The invention discloses a preparation method and application of a compounded Pu'er tea functional composition and belongs to the field of biotechnology and medicines. Effective parts of Pu'er tea, dendrobium nobile and morus plants are subjected to the steps of water extraction, alcohol extraction, alcohol precipitation, organic solvent extraction, concentration, drying and the like to obtain extracts, and the extracts are compounded to obtain a composition capable of improving diseases induced by high-fat and high-sugar diet. The composition disclosed by the invention can be used for improving hyperglycemia, hyperlipidemia and non-alcoholic fatty liver disease induced by the high-fat and high-sugar diet; and compared with single use of the Pu'er tea extract, the effect is obvious, and the application value is obvious. Raw materials in compounded functional food all belong to medicine-food homologous plants, so that the compounded functional food is green and healthy, is low in side effect, good in safety and easy to industrialize and has obvious social benefits and economic values.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and discloses a preparation method of a compound Pu'er tea functional composition and its application in improving high-fat and high-sugar diet-induced diseases. Background technique [0002] With the development of society and the improvement of people's living standards, the intake of high-fat and high-sugar diets is increasing year by year. Long-term consumption of high-fat and high-sugar foods is likely to have a comprehensive impact on the human body, making the body in a sub-healthy state. A high-fat and high-sugar diet can lead to fat accumulation, obesity, and increase the content of triglycerides, total cholesterol and low-density lipoprotein in the blood, causing hyperlipidemia such as atherosclerosis or cardiovascular and cerebrovascular diseases. A high-fat and high-sugar diet can also lead to an increase in blood sugar levels, leading to an increase in the load on the liver,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/18A23F3/22A23F3/30A61K36/82A61P3/10A61P3/06A61P1/16A61K31/715
CPCA23F3/18A23F3/22A23F3/30A61K36/82A61K31/715A61K36/605A61P3/10A61P3/06A61P1/16A61K2236/331A61K2236/39A61K2236/333A61K2236/35A61K2236/51
Inventor 董悦生何良宇修志龙王银波虞诗强余洋杨睿
Owner DALIAN UNIV OF TECH
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