Grain-saving unconventional diet bio-fermentation feed production device and preparation method thereof
The technology of biological fermentation feed and production device is applied in the field of grain-saving unconventional ration biological fermentation feed production device and its preparation field. , the effect of reducing the cost of feed production
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Embodiment 1
[0045] Such as Figure 1-8 As shown, a grain-saving unconventional ration bio-fermented feed production device and its preparation method include sequentially connected storage bins 1, transition bins 3, collection bins 4, distribution bins 6 and a plurality of mixing bins 9;
[0046] The storage silo 1 is divided into a plurality of sub-silos 2 by partitions, the bottom of the sub-silos 2 is connected with a discharge port 11, and the discharge port 11 is connected with a solenoid valve discharge pipe 12, and the solenoid valve The discharge pipe 12 extends into the transition chamber 3, and the opening and closing state of the electromagnetic valve discharge pipe 12 is configured to be opposite to the opening and closing state of the material door 29 of the lower material barrel 15;
[0047] The bottom of the electromagnetic valve discharge pipe 12 is provided with a fixed frame 13, and the fixed frame 13 is fixedly connected with the wall of the transition warehouse 3, and ...
Embodiment 2
[0083] (1) Unconventional raw material preparation:
[0084] Picking fresh and tender stem and leaf hybrid mulberry trees with a height of 0.8-1.2m and 15-20cm from the root, the variety is the hybrid mulberry tree 101 of the Institute of Botany, Chinese Academy of Sciences, after removing impurities such as yellowing, rot and deterioration, moldy stems and leaves, and cleaning mud, sand and gravel , after controlling the water (drying), smash it with a green fodder beater for later use.
[0085] Pick wormwood stems and leaves with a height of 0.5-0.8m and 5-10cm from the root from April to May, remove yellowing, rotten, moldy stems and leaves, clean mud, sand and gravel and other impurities, control water (dry) and beat with green fodder The machine is smashed for spare.
[0086] The leftovers without mildew and deterioration, silt, and impurities left after processing green tea from tea factories are selected for crushing, and are set aside after passing through a 60-mesh s...
Embodiment 3
[0091] (1) Preparation of Bacillus subtilis fermentation broth:
[0092] Get 2ml Bacillus subtilis (viable bacteria concentration is 10 10 CFU / ml), inoculated in 100ml medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 7.2, the rotation speed is 200-300r / min, and the fermentation time is 24-36h. The shake flask fermentation medium is LB medium, the ingredients are: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000ml, and the pH value is 7.2.
[0093] After the shake flask fermentation is completed, carry out the expansion culture of the fermenter. Take 100ml of the shake flask fermentation seed liquid and inoculate it into a 10L fermenter with a liquid volume of 5L. min, ferment for 24-36 hours. The composition of the medium in the pilot test of the fermenter was the same as that of the shake flask fermentation medium. After the fermentation, the medium was kept at 4°C for later use.
[0094] (2) Pre...
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