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Application of Fe3O4 nano particles to improvement of fermentation performance of saccharomyces cerevisiae

A nanoparticle and Saccharomyces cerevisiae technology, applied in the field of bioengineering, can solve problems such as poor ethanol tolerance, achieve the effects of improving tolerance, accelerating oxidation decomposition, and improving catalytic efficiency

Active Publication Date: 2021-11-26
LUZHOU LAOJIAO CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is the poor tolerance of Saccharomyces cerevisiae to ethanol

Method used

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  • Application of Fe3O4 nano particles to improvement of fermentation performance of saccharomyces cerevisiae
  • Application of Fe3O4 nano particles to improvement of fermentation performance of saccharomyces cerevisiae
  • Application of Fe3O4 nano particles to improvement of fermentation performance of saccharomyces cerevisiae

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The aforementioned Big-Fe 3 o 4 The preparation method is: according to the mass ratio of ferric chloride: trisodium citrate: ethylene glycol: sodium acetate=13:4:440:24, after ferric chloride and trisodium citrate are completely dissolved in ethylene glycol , then add sodium acetate and stir vigorously until the mixture is uniform, then transfer the mixture to an autoclave, heat at 200°C for 10h, wash the product with ethanol and dry it to obtain Big-Fe 3 o 4 . Among them, sodium acetate plays the role of electrostatic balance and coordinating reducing agent in the whole system. After other raw materials are mixed, adding solid sodium acetate can make the concentration of reactants more uniform.

[0037]In order to obtain better experimental results, it is preferred that the vigorous stirring time is 30-40 minutes, the autoclave is a Teflon-lined stainless steel autoclave, and the drying temperature is 55-65°C.

[0038] The above His-Fe 3 o 4 The preparation meth...

Embodiment 1

[0051] (3) Embodiment 1 cultivation experiment

[0052] The above-prepared Small-Fe 3 o 4 , Big-Fe 3 o 4 , His-Fe 3 o 4 Take 400 μL of each stock solution and add them to 100mL LYPD medium (the main components of the medium are: 2g peptone, 2g glucose, 1g yeast extract, 2g agar) as the three experimental groups, and another 100mLYPD medium without adding Fe 3 o 4 NPs were used as the control group (NC), and then 8% (v / v) ethanol and 5% (v / v) Saccharomyces cerevisiae were added to the experimental group and the control group respectively, and cultured for 24h (30°C, 120rpm).

[0053] Result detection: In the process of co-cultivation, the optical density of Saccharomyces cerevisiae in the culture solution was detected at 600nm by using a UV-visible spectrophotometer, and the results were as follows: image 3 shown by image 3 It can be seen that the addition of His-Fe in the experimental group 3 o 4 The yeast optical density of the group was significantly higher than...

Embodiment 2

[0054] (4) Example 2 drop plate experiment

[0055] A. Small-Fe 3 o 4 , Big-Fe 3 o 4 , His-Fe 3 o 4 Take 400 μL of each stock solution, add it to 100mL LYPD solid medium (2% agar) as the experimental group, and take another 100mLYPD solid medium without adding Fe 3 o 4 NPs were used as the control group (NC), and then 12% (v / v) ethanol was added to the experimental group and the control group, respectively, for standby;

[0056] B. Small-Fe 3 o 4 , Big-Fe 3 o 4 , His-Fe 3 o 4 Take 400 μL of each stock solution, add it to 100mL LYPD solid medium (2% agar) as the experimental group, and take another 100mLYPD solid medium without adding Fe 3 o 4 NPs were used as the control group (NC), and then 16% (v / v) ethanol was added to the experimental group and the control group, respectively, for later use;

[0057] C. Drop plate test: dilute the yeast concentration to 10 0 、10 -1 、10 -2 、10 -3 and 10 -4 , pipette 10 μL dropwise into the solid medium prepared in step...

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Abstract

The invention discloses application of Fe3O4 nano particles to improvement of fermentation performance of saccharomyces cerevisiae, and belongs to the field of bioengineering, the field of food microorganisms and the field of industrial microorganisms. The application of improving the fermentation performance of the saccharomyces cerevisiae mainly comprises the application of improving the ethanol tolerance of the saccharomyces cerevisiae and the application of improving the growth rate of the saccharomyces cerevisiae. The Fe3O4 nano particles are any one of Small-Fe3O4 with the particle size of 10 to 50 nm, Big-Fe3O4 with the particle size of 250 to 450 nm, and histidine modified His-Fe3O4 with the particle size of 50 to 100 nm. According to the application of the Fe3O4 nano particles to improvement of the fermentation performance of the saccharomyces cerevisiae, the problem that the saccharomyces cerevisiae is poor in ethanol tolerance can be effectively solved.

Description

technical field [0001] The invention belongs to the fields of bioengineering, food microorganisms and industrial microorganisms, and relates to a method for improving the fermentation performance of Saccharomyces cerevisiae. Background technique [0002] Saccharomyces cerevisiae is widely used in industrial fields such as the production of alcohol, food and fuel alcohol. However, due to the continuous production of product ethanol during the fermentation process of yeast, the cells are faced with high concentration of ethanol in the final stage of fermentation, which leads to the weakening of multiple functions of the cells due to toxicity, inhibits its absorption and decomposition of glucose, and reduces the activity of intracellular glycolytic enzymes , and damage the integrity of the cell membrane, slowing down the production fermentation process, thereby affecting the fermentation efficiency, sustainability and ethanol production of yeast. [0003] At present, the main ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/54C01G49/08C12N1/38C12N1/18C12R1/865
CPCC12P7/06C12P7/54C01G49/08C12N1/38C12N1/18C01P2004/62C01P2004/04C01P2004/51Y02E50/10
Inventor 杨粟平李勇侯长军霍丹群张宿义杨平周军黄锐
Owner LUZHOU LAOJIAO CO LTD
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