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Stem cell skin care product

A technology of stem cells and adipose stem cells, applied in the field of bio-cosmetology, can solve the problems that the effect needs to be further strengthened, the mechanism of action is single, the aggregation of exosome vesicles, fusion affects the efficacy, etc., to reduce pigmentation, promote release, and protect the effect. Effect

Pending Publication Date: 2021-12-03
安徽科门生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ordinary growth factor oligopeptide products have a single mechanism of action, and the defects of exosome vesicles such as aggregation and fusion affect the efficacy, and the effect needs to be further strengthened

Method used

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  • Stem cell skin care product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of stem cell skin care products:

[0024] 1. Adipose stem cell culture: Collect and clean the adipose tissue, mince it, add type Ⅰ collagenase to digest until it becomes a paste, filter, add complete medium to stop digestion, centrifuge at 1000rpm for 10min, discard the supernatant, and keep the lower layer of cell pellet ; Resuspend the inoculation culture with the complete medium, and subculture when the cell growth reaches 80-90% confluence;

[0025] 2. Preparation of chitosan-adipocyte exosome complexes: when the adipose stem cells were cultured to P4 generation, when the fusion rate reached 85%, they were cultured with complete medium with exosomes removed, and 0.1wt% chitosan was added at the same time Sugar, collect the supernatant after culturing for 48 hours, centrifuge at 2000g at 4°C for 15min, take the supernatant, then centrifuge at 3000g at 4°C for 20min, take the supernatant, add ExoQuick-TC exosome precipitation solution, refrigerate overnigh...

Embodiment 2

[0030] Preparation of stem cell skin care products:

[0031] 1. Adipose stem cell culture: Collect and clean the adipose tissue, mince it, add type Ⅰ collagenase to digest until it becomes a paste, filter, add complete medium to stop digestion, centrifuge at 1000rpm for 10min, discard the supernatant, and keep the lower layer of cell pellet ; Resuspend the inoculation culture with the complete medium, and subculture when the cell growth reaches 80-90% confluence;

[0032] 2. Preparation of chitosan-adipocyte exosome complex: when the adipose stem cell is cultured to the P4 generation, when the fusion rate reaches 85%, it is cultured with a complete medium that removes exosomes, and 0.05wt% chitosan is added at the same time Sugar, collect the supernatant after culturing for 48 hours, centrifuge at 2000g at 4°C for 15min, take the supernatant, then centrifuge at 3000g at 4°C for 20min, take the supernatant, add ExoQuick-TC exosome precipitation solution, refrigerate overnight, ...

Embodiment 3

[0037] Preparation of stem cell skin care products:

[0038] 1. Adipose stem cell culture: Collect and clean the adipose tissue, mince it, add type Ⅰ collagenase to digest until it becomes a paste, filter, add complete medium to stop digestion, centrifuge at 1000rpm for 10min, discard the supernatant, and keep the lower layer of cell pellet ; Resuspend the inoculation culture with the complete medium, and subculture when the cell growth reaches 80-90% confluence;

[0039]2. Preparation of chitosan-adipocyte exosome complex: when the adipose stem cell is cultured to the P4 generation, when the fusion rate reaches 85%, it is cultured with a complete medium that removes exosomes, and 0.2wt% chitosan is added at the same time Sugar, collect the supernatant after culturing for 48 hours, centrifuge at 2000g at 4°C for 15min, take the supernatant, then centrifuge at 3000g at 4°C for 20min, take the supernatant, add ExoQuick-TC exosome precipitation solution, refrigerate overnight, an...

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Abstract

The invention discloses a stem cell skin care product, and relates to the technical field of biological beauty. The stem cell skin care product is prepared from the following raw materials: adipose-derived stem cell exosome freeze-dried powder and a solvent, wherein the adipose-derived stem cell exosome freeze-dried powder is prepared from the following raw materials through freeze drying: a chitosan-adipose-derived stem cell exosome compound and a freeze-drying agent; and the solvent is prepared from arbutin, hyaluronic acid, total saponins of ginseng, radix scutellariae extract, glycerol, butanediol and de-ionized water. The skin care product provided by the invention has the synergistic effect of repairing, nourishing and activating, and has the effects of delaying skin ageing from the source, improving skin problems including dark yellow, rough skin and the like, fading spots and brightening, fading fine wrinkles, refining pores, repairing hyperplastic scars, improving skin sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of biological beauty, in particular to a stem cell skin care product. Background technique [0002] With the continuous improvement of living standards, people's requirements for quality of life are also gradually increasing, and the demand for delaying aging and maintaining youth is particularly important. As the largest tissue and organ of the human body, the skin covers the appearance of the human body and shows people's appearance. The use of cosmetics can achieve the purpose of improving skin problems by covering up defects or acting on the surface of the skin, but the effect is not significant, and it cannot fundamentally improve skin problems such as dullness and aging. [0003] Studies have shown that the self-renewal, regeneration, repair, and aging degeneration of skin epidermal cells are determined by the proliferation and differentiation of adult stem cells from hair follicles or the basal layer...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/34A61K8/60A61K8/73A61K8/9789A61Q19/02A61Q19/08C12N5/0775
CPCA61K8/99A61K8/9789A61K8/602A61K8/735A61K8/60A61K8/345A61Q19/02A61Q19/08C12N5/0667A61K2800/84C12N2509/00
Inventor 张正亮
Owner 安徽科门生物科技有限公司
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