Phaffia rhodozyma strain with high yield of astaxanthin and breeding method and application of phaffia rhodozyma strain

A technology of Phaffia rhodozyma and yeast strains, which is applied in the field of high-yield astaxanthin-producing Phaffia rhodozyme strains and its breeding field, can solve the problems of low astaxanthin content, long cycle, pollution, etc., and achieve a remarkable mutagenic effect , increase the effect of filtering to, large application prospects

Active Publication Date: 2021-12-14
LUCNOVA BIO TECH CO LTD HUBEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the price of chemically synthesized astaxanthin is relatively cheap, and it still has certain competitiveness in feed additives, but its structure is mostly cis, and the animal utilization rate is low. Threat; For countries with developed shrimp and crab processing industries, crustacean waste is a good source of astaxanthin, but its astaxanthin content is very low, organic solvent pollution in the extraction process, low product purity and other problems It limits its further development; the content of astaxanthin in Haematococcus pluvialis generally accounts for 0.2%-2% of dry cell weight, up to 4%. It is an excellen...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The method for breeding a Phaffia rhodozyme strain with high astaxanthin production, the specific operation steps are as follows:

[0056] (1) Activation of bacteria

[0057] Dissolve the freeze-dried powder of Phaffia rhodozyme in sterile water, inoculate all of it on the slope of YPDA test tube or on the plate of the petri dish, cultivate it at 22°C for 4 days, and then pick a ring of bacteria from the activated slope and inoculate it into the shake flask , cultured at 21°C for 48 hours at a speed of 180rpm, and stored in a refrigerator at 4°C to obtain activated strains;

[0058] Wherein, the culture medium of shaking flask is YPD (yeast extract powder peptone glucose) agar medium, specifically glucose 20g / L, peptone 20g / L, yeast powder 10g / L, agar 20g / L, pH is 6.4;

[0059] (2) Preparation of logarithmic growth phase bacterial liquid

[0060] The activated bacterial classification is inoculated into another shake flask from step (1), measures its growth curve, fin...

Embodiment 2

[0073] The method for breeding a Phaffia rhodozyme strain with high astaxanthin production, the specific operation steps are as follows:

[0074] (1) Activation of bacteria

[0075] Dissolve the lyophilized powder of Phaffia rhodozyma in sterile water, inoculate all of it on the slant of YPDA test tube or on the plate of the petri dish, cultivate it at 20°C for 5 days, and then pick a ring of bacteria from the activated slant and inoculate it into the shake flask , cultured at 20°C for 60 hours at a speed of 200rpm, and stored in a refrigerator at 4°C to obtain activated strains;

[0076] Wherein, the culture medium of shaking flask is YPD (yeast extract powder peptone glucose) agar medium, specifically glucose 20g / L, peptone 20g / L, yeast powder 10g / L, agar 20g / L, pH is 6.4;

[0077] (2) Preparation of logarithmic growth phase bacterial liquid

[0078] The activated bacterial classification is inoculated into another shake flask from step (1), measures its growth curve, find...

Embodiment 3

[0091] The method for breeding a Phaffia rhodozyme strain with high astaxanthin production, the specific operation steps are as follows:

[0092] (1) Activation of bacteria

[0093] Dissolve the freeze-dried powder of Phaffia rhodozyma in sterile water, inoculate all of it on the slope of YPDA test tube or on the plate of the petri dish, cultivate it at 25°C for 3 days, and then pick a ring of bacteria from the activated slope and inoculate it into the shake flask , cultured at 22°C for 36 hours at a speed of 150rpm, and stored in a refrigerator at 4°C to obtain activated strains;

[0094] Wherein, the culture medium of shake flask culture is YPD (yeast extract powder peptone glucose) medium, specifically glucose 20g / L, peptone 20g / L, yeast powder 10g / L, pH is 6.4;

[0095] (2) Preparation of logarithmic growth phase bacterial liquid

[0096] The activated bacterial classification is inoculated into another shake flask from step (1), measures its growth curve, finds that the l...

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PUM

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Abstract

The invention discloses a phaffia rhodozyma strain for high yield of astaxanthin, which is a Phaffiarhodozyma LUCNOVA001 strain, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M20211124, has the preservation date of September 1, 2021, and is named as PhaffiarhodozymaLUCNOVA001 in taxonomy; the strain is obtained by taking Phaffiarhodozyma as a starting production strain and carrying out ARTP mutagenesis and [beta]-ionone screening, and the breeding method comprises the following steps: (1) activating the strain; (2) preparing bacterial liquid in a logarithmic phase; (3) preparing a bacterial suspension; (4) carrying out ARTP mutagenesis; (5) screening the [beta]-violet ketone; (6) secondary screening of strains; and (7) performing continuous passage. The astaxanthin yield and content of the strain and the corresponding biomass meet the requirements of industrial production, and the strain has a great application prospect.

Description

technical field [0001] The invention relates to the technical field of strain breeding, and more specifically relates to a Phaffia rhodozyme strain with high astaxanthin production, a breeding method and application thereof. Background technique [0002] Astaxanthin, also known as astaxanthin, astaxanthin, lobster shell pigment, etc., is naturally purple-red and widely exists in the biological world, especially in crustaceans, salmon, rainbow trout, etc., and is their main component. Pigment (1. Johnson E A, AnG H. Anstaxanthin from microbial sources. Critical Reviews in Biotechnology, 1991.11: 297-326; 2. Torrissen O J, Christiansen R. Requirements for carotenoidsin fish diets. J Appl Ichthyol., 1995.11: 225-230 3. Pharmaceuticals M. Aquaxan TMHD algal meal use in aquaculture diets: Enhancing nutritional performance and pigmentation. Inc. Technical Report TR, 1999.2102.001). [0003] The chemical name of astaxanthin is 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, and the mo...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N15/01C12P23/00C12R1/645
CPCC12N13/00C12N15/01C12P23/00
Inventor 李英俊李双全胡志琦顿耀豪夏珂梁运祥李斯泉
Owner LUCNOVA BIO TECH CO LTD HUBEI
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