Method for improving salt stress resistance of salvia miltiorrhiza

A technology of salt stress and salvia miltiorrhiza, applied in the field of improving salt stress resistance of salvia miltiorrhiza, to reduce the use of herbicides, increase biomass, and protect the ecological environment

Pending Publication Date: 2021-12-31
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are very few existing plant growth regulators that can improve the resistance of Salvia miltiorrhiza to salt stress, so it is necessary to explore more p

Method used

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  • Method for improving salt stress resistance of salvia miltiorrhiza
  • Method for improving salt stress resistance of salvia miltiorrhiza
  • Method for improving salt stress resistance of salvia miltiorrhiza

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0024] Example 1

[0025] 1 Preparation of different concentrations 5-aminoethyl propacionic acid

[0026] 5-aminoethylene propylene propylene antibody was protected from distilled water to 0, 10, 20 mg / lala mediation.

[0027] Table 1 5-concentration gradient of aminoethyl propionic acid

[0028]

[0029] Note: The recommended concentration refers to the optimum concentration obtained after the test, namely: the ALA content added in each 1L water.

[0030] 2 materials and squares

[0031] 2.1 Precession and Germination of Salvia Seeds

[0032] The full healthy salvia seed was selected, soaked with distilled water for 10 min, then the soaked seed was sterilized for 30 seconds with a 70% ethanol solution, then washed, then cultured in a culture dish containing two-layer filter paper at 25 ° C for 72 h. The buds in the dark are 5 days buds, roots.

[0033] 2.2 Dan Participation in the establishment of a salt co-cultivation system

[0034] Seeds of germinated seeds into the matri...

Example Embodiment

[0043] Example 2 Antioxidant enzyme assay

[0044] Activity of antioxidant enzymes includes superoxide dismutase (SOD), peroxidase (POD), hydrogen peroxidase (CAT) and ascorbate peroxidase (APX), anti-oxidase activity MUTI-MODE READER (SYNERGYHTX) The instrument measurements according to the following method.

[0045] 1. SOD enzyme measurement

[0046] SOD activity assay NBT method (ZHANG et al, 2008). A total of 3 ml of the reaction system contains 50 mM phosphate buffer (pH 7.8), 13 mM L-methionine, 75 μM Nuke, and 100 μl of enzyme extract. The reaction mixture was reacted at 4000 LX for 20 min, which was more than 560 nm wavelength to inhibit 50% of NBT photochemical reduction to calculate SOD activity.

[0047] 2.POD measurement

[0048] POD activity assay adopts a healing woodphenoxide (Zhou and Leul, 1999). The reaction system has a total of 3 m1, containing 50 mM phosphate buffer (pH 6.0), 0.3% caterphenol, 0.4% h 2 O 2 And 100 μl of enzyme extract. The increase in absorban...

Example Embodiment

[0059] Determination of ROS and 3 MDA Example

[0060] MDA was measured using a spectrophotometer and ROS (UV-5500, METASH).

[0061] Determination of 1.MDA

[0062] Determination of the MDA (1999) of thiobarbituric acid colorimetric method modified according to Zhou and Leul. The supernatant enzyme solution was added 2mL 5mL 0.5% of thiobarbituric acid (TBA, 10% trichloroacetic acid formulation) in a water bath at 95 deg.] C the reaction 30min, the ice bath immediately; 5000g centrifugation 10min, 532nm and 600nm in the supernatant at colorimetric, for calculating a difference between the content of MDA, extinction coefficient 155mM -1 cm -1 .

[0063] Determination of 2.ROS

[0064] Reactive oxygen species (ROS) include hydrogen peroxide (H 2 O 2 ), Superoxide anion (O 2 - ),Hydroxyl radicals( - OH).

[0065] 2.1 H 2 O 2 Determination

[0066] Hide 2 O 2 It determined according Velikova et al. (2000), the reaction system 2mL, 0.5mL containing supernatant, 10mM of potassium phos...

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Abstract

The invention discloses a method for improving salt stress resistance of salvia miltiorrhiza. The method comprises the step of applying 5-aminolevulinic acid to salvia miltiorrhiza leaves. The invention provides a new application of 5-aminolevulinic acid in improving salt stress of salvia miltiorrhiza, and further finds that 5-aminolevulinic acid improves salt resistance by improving biomass of salvia miltiorrhiza, promoting synthesis of antioxidant enzymes in salvia miltiorrhiza plants and reducing levels of MDA and ROS in the salvia miltiorrhiza plants. The problem that the growth of salvia miltiorrhiza is influenced by salt stress in the salvia miltiorrhiza planting process is effectively relieved, the use of herbicides is greatly reduced, and the ecological environment is protected.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for improving salt stress resistance of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza Bunge, Lamiaceae is a Chinese herbal medicine widely used in the treatment of cardiovascular and cerebrovascular diseases, chronic renal failure, neurasthenia insomnia, dysmenorrhea and liver cirrhosis, and the plant has also been developed into various preparations such as The famous Compound Danshen Dripping Pills has been widely used in many countries. At present, there have been many reports on the resistance research of Danshen, such as: [0003] Plant growth regulators (PGRs) are widely used to enhance plant stress resistance. One of the PGRs is 5-aminolevulinic acid (ALA), which is a key precursor for the biosynthesis of all porphyrin compounds, such as chlorophyll, heme, etc. As a new plant growth regulator, it can participate in the regulation ...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01G7/06A01P21/00
CPCA01N37/44A01G7/06
Inventor 许玲李欣潘建民李娟娟侯卓妮孙月娥
Owner ZHEJIANG SCI-TECH UNIV
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