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Method for improving salt stress resistance of salvia miltiorrhiza

A technology of salt stress and salvia miltiorrhiza, applied in the field of improving salt stress resistance of salvia miltiorrhiza, to reduce the use of herbicides, increase biomass, and protect the ecological environment

Pending Publication Date: 2021-12-31
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are very few existing plant growth regulators that can improve the resistance of Salvia miltiorrhiza to salt stress, so it is necessary to explore more plant growth regulators to solve the biological adversity caused by salt stress to Salvia miltiorrhiza and improve the mitigation of Salvia miltiorrhiza. Effects of Salt Stress

Method used

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  • Method for improving salt stress resistance of salvia miltiorrhiza
  • Method for improving salt stress resistance of salvia miltiorrhiza
  • Method for improving salt stress resistance of salvia miltiorrhiza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1 Preparation of different concentrations of 5-aminolevulinic acid

[0026] Weigh 5-aminolevulinic acid in the dark, and prepare 0, 10, 20mg / LALA mediation solution with distilled water.

[0027] Table 1 Concentration gradient of 5-aminolevulinic acid

[0028]

[0029] Note: The recommended concentration refers to the optimal concentration obtained after passing the test, that is, the ALA content added per 1L of water.

[0030] 2 Materials and recipes

[0031] 2.1 Pretreatment and germination of Salvia miltiorrhiza seeds

[0032] Select plump and healthy Salvia miltiorrhiza seeds, soak them in distilled water for 10 minutes, then sterilize the soaked seeds with 70% ethanol solution for 30 seconds, wash them, and then culture them in a petri dish containing two layers of filter paper under dark conditions at 25°C for 72 hours Germinate in the dark for 5 days to accelerate germination and take root.

[0033] 2.2 Establishment of dandelion participation in salt co-c...

Embodiment 2

[0043] Example 2 Determination of antioxidant enzymes

[0044] The activity of antioxidant enzymes includes superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and the activity of antioxidant enzymes is measured with Muti-Mode Reader (SYNERGYHTX) The instrument measures according to the following method.

[0045] 1. Determination of SOD enzyme

[0046] SOD activity was determined by NBT method (Zhang et al., 2008). The total reaction system was 3 mL, including 50 mM phosphate buffer (pH7.8), 13 mM L-methionine, 75 μM NBT, 0.1 mM EDTA, 2 μM riboflavin and 100 μl enzyme extract. The reaction mixture was reacted under the condition of 4000lx for 20min, and the color was measured at a wavelength of 560nm, and the SOD activity was calculated by taking 50% inhibition of NBT photoreduction as an enzyme activity unit.

[0047] 2. Determination of POD

[0048] POD activity was measured by guaiacol oxidation method (Zhou and Leul, 1999). The...

Embodiment 3

[0059] The measurement of embodiment 3 MDA and ROS

[0060] MDA and ROS were measured with a spectrophotometer (UV-5500, METASH).

[0061] 1. Determination of MDA

[0062] MDA content was determined according to Zhou and Leul (1999) with modified thiobarbituric acid colorimetric method. Add 5mL of 0.5% thiobarbituric acid (TBA, prepared with 10% trichloroacetic acid) solution to 2mL supernatant enzyme solution, react in a water bath at 95°C for 30min, and immediately put it in an ice bath; centrifuge at 5000g for 10min, and take the supernatant at Colorimetric at the place, the difference is used to calculate the MDA content, the extinction coefficient is 155mM -1 cm -1 .

[0063] 2. Determination of ROS

[0064] Reactive oxygen species (ROS) including hydrogen peroxide (H 2 o 2 ), superoxide anion (O 2 - ),Hydroxyl radicals( - OH).

[0065] 2.1H 2 o 2 Determination of

[0066] h 2 o 2 According to the method of Velikova et al. (2000), the reaction system was 2...

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Abstract

The invention discloses a method for improving salt stress resistance of salvia miltiorrhiza. The method comprises the step of applying 5-aminolevulinic acid to salvia miltiorrhiza leaves. The invention provides a new application of 5-aminolevulinic acid in improving salt stress of salvia miltiorrhiza, and further finds that 5-aminolevulinic acid improves salt resistance by improving biomass of salvia miltiorrhiza, promoting synthesis of antioxidant enzymes in salvia miltiorrhiza plants and reducing levels of MDA and ROS in the salvia miltiorrhiza plants. The problem that the growth of salvia miltiorrhiza is influenced by salt stress in the salvia miltiorrhiza planting process is effectively relieved, the use of herbicides is greatly reduced, and the ecological environment is protected.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for improving salt stress resistance of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza Bunge, Lamiaceae is a Chinese herbal medicine widely used in the treatment of cardiovascular and cerebrovascular diseases, chronic renal failure, neurasthenia insomnia, dysmenorrhea and liver cirrhosis, and the plant has also been developed into various preparations such as The famous Compound Danshen Dripping Pills has been widely used in many countries. At present, there have been many reports on the resistance research of Danshen, such as: [0003] Plant growth regulators (PGRs) are widely used to enhance plant stress resistance. One of the PGRs is 5-aminolevulinic acid (ALA), which is a key precursor for the biosynthesis of all porphyrin compounds, such as chlorophyll, heme, etc. As a new plant growth regulator, it can participate in the regulation ...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01G7/06A01P21/00
CPCA01N37/44A01G7/06
Inventor 许玲李欣潘建民李娟娟侯卓妮孙月娥
Owner ZHEJIANG SCI-TECH UNIV
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