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Preparation method of biomedical tissue adhesive

A tissue adhesive and biomedical technology, applied in the field of clinical medicine, can solve the problems of poor adhesion strength, high cytotoxicity, easy to fall off, etc., and achieve good adhesion performance, simple gelation process, and high adhesion strength.

Pending Publication Date: 2021-12-31
上海曜爱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of high cytotoxicity of most existing synthetic adhesives, the present invention provides a preparation method of oxidized dextran / ε-polylysine hydrogel tissue adhesive, the selection of materials is green and environmentally friendly, and the product is cytotoxic Low
[0006] Aiming at the problems that existing natural tissue adhesives have good biocompatibility but poor adhesion strength, and are easy to fall off in actual clinical applications, the present invention uses green and environmentally friendly dextran and ε-polylysine to prepare a product with excellent adhesion strength composite tissue adhesive
[0007] Aiming at the problems of long preparation process and uncontrollable product properties of composite tissue adhesives, the present invention provides a simple and controllable process to prepare hydrogel adhesives

Method used

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  • Preparation method of biomedical tissue adhesive
  • Preparation method of biomedical tissue adhesive
  • Preparation method of biomedical tissue adhesive

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Prepare an aqueous solution of dextran with a mass fraction of 20%, mix it with an aqueous solution of sodium periodate with a mass fraction of 1%, control the volume ratio of the two to 2:1, and finally stir at a constant temperature at 30°C for 60 minutes; After the reaction is over, dialyze the mixed solution with running water for 18 hours, then further dialyze the mixed solution with deionized water twice for 1.5 hours, and finally obtain aldehyde dextran by drying, and grind it into powder Carry out vacuum drying for 24 hours, and refrigerate and store at -15°C until use;

[0024] (2) Preparation of ε-polylysine aqueous solution with a mass fraction of 25%, diluting acetic anhydride to a concentration of 6% aqueous acetic anhydride with deionized water, mixing the two solutions at a volume ratio of 2:1, at a constant temperature The reaction was carried out at 45°C for 60 minutes under stirring conditions. After the reaction, the reaction solution was purified...

Embodiment 2

[0027] (1) Prepare an aqueous solution of dextran with a mass fraction of 20%, mix it with an aqueous solution of sodium periodate with a mass fraction of 1%, control the volume ratio of the two to 2:1, and finally stir at a constant temperature of 70°C for 60 minutes; After the reaction is over, dialyze the mixed solution with running water for 18 hours, then further dialyze the mixed solution with deionized water twice for 1.5 hours, and finally obtain aldehyde dextran by drying, and grind it into powder Carry out vacuum drying for 24 hours, and refrigerate and store at -15°C until use;

[0028] (2) Preparation of ε-polylysine aqueous solution with a mass fraction of 25%, diluting acetic anhydride to a concentration of 6% aqueous acetic anhydride with deionized water, mixing the two solutions at a volume ratio of 2:1, at a constant temperature The reaction was carried out at 45°C for 60 minutes under stirring conditions. After the reaction, the reaction solution was purified...

Embodiment 3

[0031] (1) Prepare a dextran aqueous solution with a mass fraction of 20%, mix it with a 20% sodium periodate aqueous solution, control the volume ratio of the two to 2:1, and finally stir at 30°C for 60 minutes at a constant temperature; After the reaction is over, dialyze the mixed solution with running water for 18 hours, then further dialyze the mixed solution with deionized water twice for 1.5 hours, and finally obtain aldehyde dextran by drying, and grind it into powder Carry out vacuum drying for 24 hours, and refrigerate and store at -15°C until use;

[0032] (2) Preparation of ε-polylysine aqueous solution with a mass fraction of 25%, diluting acetic anhydride to a concentration of 6% aqueous acetic anhydride with deionized water, mixing the two solutions at a volume ratio of 2:1, at a constant temperature The reaction was carried out at 45°C for 60 minutes under stirring conditions. After the reaction, the reaction solution was purified by dialysis for 24 hours with ...

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Abstract

The invention discloses a preparation method of a biomedical tissue adhesive. Hydrogel is prepared by carrying out nucleophilic addition reaction on an aldehyde group of oxidized dextran and an amino group of epsilon-polylysine, and the controllability of gelling time and adhesive strength is realized by regulating and controlling the oxidation degree of dextran and the acylation degree of epsilon-polylysine. The obtained biomedical tissue adhesive has low cytotoxicity and good adhesion performance, and the gel forming process is simple and controllable.

Description

technical field [0001] The invention belongs to the field of clinical medicine, in particular to a preparation method of biomedical tissue adhesive. Background technique [0002] Currently, surgical sutures, rivets, medical tape and other supplies are often used for mechanical fixation in the repair of wounds. These mechanical fasteners must firmly fix the connected wound tissue and prevent the leakage of gas, blood and interstitial fluid. At the same time, the fastener needs to resist a certain mechanical tensile load to achieve the purpose of fully healing the wound and restoring its structure and function. Although the application of these traditional methods is very mature, their limitations can no longer meet the increasing clinical needs, such as the time-consuming suture process, the risk of infection caused by removing stitches and changing dressings, and the aesthetic problems of easily leaving scars. [0003] With the continuous development of modern medical techn...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08G81/00A61L24/00A61L24/04
CPCC08J3/075C08G81/00A61L24/046A61L24/0031C08L87/005
Inventor 孙乐青周慧丽张婷婷赵瑾柳仁民
Owner 上海曜爱生物科技有限公司
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