Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glucohexaose and preparation method and application thereof, and hair regeneration preparation

A technology of glucomannan and glucomannan, applied in the field of hair regeneration preparation, glucomannan, can solve the problems of recurrence of hair loss, low efficiency and high cost, and achieves the treatment of hair loss and the promotion of periodicity. effect of regeneration

Active Publication Date: 2022-01-04
UNIVERSITY OF MACAU
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the FDA-approved topical drug-minoxidil and oral drug-finasteride can effectively improve androgenetic alopecia to a certain extent, but there are problems such as low effective rate and recurrence of hair loss after drug withdrawal.
At the same time, although hair transplantation can be used as an alternative treatment, its invasiveness, limited number of autologous hair follicle donors, low survival rate of hair follicle transplantation, and high cost limit its further application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glucohexaose and preparation method and application thereof, and hair regeneration preparation
  • Glucohexaose and preparation method and application thereof, and hair regeneration preparation
  • Glucohexaose and preparation method and application thereof, and hair regeneration preparation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0037] In the second aspect, the embodiment of the present invention provides a method for preparing glucomanose, comprising:

[0038] The glucomannan raw material is dissolved in water, and then the formed glucomannan raw material solution is freeze-dried to form a freeze-dried powder.

[0039] Then, use cellulase to enzymatically degrade glucomannan, that is, the above-mentioned freeze-dried powder, specifically, fully dissolve the above-mentioned freeze-dried powder and cellulase in water, wherein the enzyme-to-bottom ratio is 1:5-1:20 , for example, the enzyme-to-substrate ratio is 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15 , 1:16, 1:17, 1:18, 1:19, 1:20, etc. Any value between 1:5-1:20. Enzyme degradation temperature is 35-50°C, for example, the temperature is 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C Any value between 35-50°C, such as ℃, 48°C, 49°C and 50°C. The enzymatic degradation time is 24-37 hours, for example,...

Embodiment 1

[0047] The embodiment of the present invention provides a preparation method of glucomanose, comprising:

[0048] Weigh 5g of konjac powder extracted from the tuber part of natural konjac plant and dissolve it completely in 100ml deionized water, stir fully for 24h, then freeze-dry the konjac polysaccharide solution to obtain konjac glucomannan jelly dry powder. Wherein, the preparation method of the konjac polysaccharide is as follows: take the rhizome part of the natural konjac, grind it with a traditional Chinese medicine grinder, and obtain konjac powder. The konjac flour is repeatedly washed with a water-containing ethanol solution to obtain crudely extracted konjac polysaccharides.

[0049] Next, the konjac polysaccharide freeze-dried powder was degraded by cellulase (purchase source: Aladdin Reagent (Shanghai) Co., Ltd.). First, 500 mg of konjac glucomannan freeze-dried powder was weighed and fully dissolved in 100 ml of deionized water, the reaction temperature was 4...

experiment example 1

[0052] Induced Stimulation Experiment

[0053] Using the glucomanose sample prepared in Example 1 above, the mouse bone marrow-derived macrophages (mBMDM, laboratory method to extract primary cells from C57BL / 6J mice) were used to induce and stimulate experiments: specifically:

[0054] Mouse bone marrow-derived mononuclear cells (5×10 6 Cells / each 10cm petri dish) were inoculated in a 10cmpetri dish, cultured with L929 cell supernatant culture fluid+RPMI-1640 medium+10% fetal bovine serum+1% penicillin-streptomycin cultured for 7 days, Placed at 37°C, 5% CO 2 After the cells adhered to the wall in the incubator, they were co-incubated with 50ug / ml hexaglucose for 24h, which was recorded as the OG6 group. Group. After co-incubating for 24 hours, TRIzol (Invitrogen) was used to extract the total RNA in the sample, and the eukaryotic mRNA sequencing experiment analysis and bioinformatics analysis were performed to compare the glucomanose-stimulated mouse bone marrow-derived m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of skin hair regeneration, in particular to glucohexaose and a preparation method and application thereof, and a hair regeneration preparation. The molecular structure of the glucohexaose is Man-Man-Man-Man-Glc-Glc. The gulcohexaose can specifically stimulate local macrophages to release a cytokine network mainly comprising CCL5 chemotactic factors, recruit lymphocytes mainly comprising regulatory T cells, induce immune cascade reaction, regulate immune microenvironment, activate rapid proliferation and differentiation of hair follicle stem cells at hair follicle carina and at the lower end of the carina, remarkably promote periodic regeneration of hair follicles and accelerate the process of driving the hair follicles to enter a growth period from a resting period, so as to achieve the effect of treating alopecia.

Description

technical field [0001] The invention relates to the technical field of skin and hair regeneration, in particular to glucomanose, a preparation method thereof, an application thereof and a hair regeneration preparation. Background technique [0002] In today's society, with the increasing pressure of people's life and the gradual increase in attention to the external image, the more and more common hair loss symptoms will not only affect the social quality of patients, but may even cause patients to have certain mental pressure. This can further develop into serious mental health problems. Healthy hair not only has important physiological functions for humans, such as protecting the skin from ultraviolet rays, draining sweat, and regulating body temperature, but also has important social functions. Therefore, researchers have been constantly trying to prevent and treat hair loss through safe and effective methods. But at present, the means of clinical treatment alopecia are...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P17/14A61K8/73A61Q7/00C12P19/14C12P19/04
CPCC08B37/006A61K31/715A61P17/14A61K8/73A61Q7/00C12P19/14C12P19/04
Inventor 王春明殷小雨王琳涛张哲
Owner UNIVERSITY OF MACAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com