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Natural polysaccharide sponge and preparation method and application thereof

A natural polysaccharide and sponge technology, applied in the field of natural polysaccharide sponge and its preparation, can solve the problem of lack of sponge and achieve the effect of excellent biocompatibility and uniform dispersion

Inactive Publication Date: 2022-01-21
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the hemostatic sponge with porous structure has attracted more and more attention. The sponge has good liquid absorption ability, can quickly absorb blood and exudate on the wound surface, effectively activate platelets, and achieve rapid hemostasis; but there is still a lack of Sponges that are degradable, safe and non-toxic, and have excellent hemostatic properties still need further research and development

Method used

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  • Natural polysaccharide sponge and preparation method and application thereof
  • Natural polysaccharide sponge and preparation method and application thereof
  • Natural polysaccharide sponge and preparation method and application thereof

Examples

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preparation example Construction

[0051] (1) Preparation of aldylated natural polysaccharides: Weigh 1 to 5 g of chondroitin sulfate, sodium hyaluronate or dextran, pour into deionized water, stir at room temperature until completely dissolved, and obtain a concentration of 1% (wt / v) polysaccharide aqueous solution; subsequently, add sodium periodate, add according to the ratio of natural polysaccharide and sodium periodate mass ratio being 1~10:1, avoid light and react for 1~3h; Then, add excess ethylene glycol, Terminate the reaction and continue to stir for 1-2 hours; finally, put the above solution into a dialysis bag, dialyze in ultrapure water for 3-6 days, and freeze-dry at -65°C to obtain the aldehydated natural polysaccharide solid powder. Refrigerate at -18°C until use.

[0052] (2) Hemolysis rate test: take rabbit arterial blood, add 20mL PBS buffer (pH 7.4) to 10mL rabbit whole blood, mix gently and centrifuge the mixture at 3000rpm for 6 minutes. Then red blood cells (RBCs) were collected from t...

Embodiment 1

[0063] Preparation of natural polysaccharide sponge:

[0064] (1) carboxymethyl chitosan and kaolin are dissolved in normal saline to obtain solution A containing 2wt.% CMCS and 0.5wt.% KA;

[0065] (2) Prepare aldylated hyaluronic acid, wherein the mass ratio of sodium hyaluronate to sodium periodate is 1:1, the dark reaction time is 2h, the molecular weight cut-off of the dialysis bag is 3500, and the aldylated hyaluronic acid Dissolved in physiological saline to obtain a solution B containing 5wt.% aldylated hyaluronic acid;

[0066] (3) Mix solution A and solution B at a volume ratio of 3:7, and gel at room temperature for 12 seconds to obtain a hydrogel;

[0067] (4) The hydrogel was frozen at -80°C for 48 hours, and then freeze-dried to obtain a natural polysaccharide sponge.

[0068] The natural polysaccharide sponge contains 14 parts by weight of carboxymethyl chitosan, 3.5 parts of kaolin, and 83 parts of aldylated hyaluronic acid. The physical figure and scanning ...

Embodiment 2

[0071] Preparation of natural polysaccharide sponge:

[0072] (1) carboxymethyl chitosan and kaolin are dissolved in normal saline to obtain solution A containing 4wt.% CMCS and 2wt.% KA;

[0073] (2) Preparation of aldylated chondroitin sulfate, wherein the mass ratio of chondroitin sulfate to sodium periodate is 3:1, the reaction time in the dark is 1h, the molecular weight cut-off of the dialysis bag is 8000-14000, and the aldylated chondroitin sulfate Dissolve chondroitin sulfate in normal saline to obtain a solution B containing 4wt.% formylated chondroitin sulfate;

[0074] (3) Mix solution A and solution B at a volume ratio of 5:5, and gel at room temperature for 125 seconds to obtain a hydrogel;

[0075] (4) The hydrogel was frozen at -80°C for 72 hours, and then freeze-dried to obtain a natural polysaccharide sponge.

[0076] The natural polysaccharide sponge contains 40 parts by weight of carboxymethyl chitosan, 20 parts of kaolin, and 40 parts of aldylated chondro...

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Abstract

The invention discloses a natural polysaccharide sponge and a preparation method and application thereof, and belongs to the field of biomedical polymer materials. The preparation method of the natural polysaccharide sponge comprises the following steps: (1) preparing a solution A containing polysaccharide I with amino and an inorganic compound; (2) preparing a solution B containing polysaccharide II with aldehyde; (3) mixing the solution A and the solution B, and carrying out a cross-linking reaction to obtain hydrogel; and (4) freezing the hydrogel at -80 DEG C to -196 DEG C for 12-72 hours, and then performing freeze drying treatment to obtain the natural polysaccharide sponge. The natural polysaccharide sponge provided by the invention has the advantages of good hemostatic effect, light weight, strong liquid absorption and lower hemolysis rate, and the hemolysis rate of the natural polysaccharide sponge is less than 5%, meeting the biological safety requirements on biological materials in the national standard.

Description

technical field [0001] The invention relates to the field of biomedical polymer materials, in particular to a natural polysaccharide sponge and its preparation method and application. Background technique [0002] Uncontrollable bleeding poses a very big threat to people's life safety. The hemostatic gauze, hemostatic bandage, hemostatic fiber and other materials commonly used in clinical practice have problems such as not being absorbed by the tissue, slow hemostasis, easy to adhere to the wound and cause secondary damage, especially for parts that cannot be pressed. The development of new fast and effective hemostatic materials can relieve the pain of patients and save the lives of the injured, which is one of the key research directions in the field of biomedical materials. [0003] In recent years, the hemostatic sponge with porous structure has attracted more and more attention. The sponge has good liquid absorption ability, can quickly absorb blood and exudate on the ...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/02A61L24/08C08J9/28C08L5/08C08L5/02
CPCA61L24/08A61L24/0036A61L24/02A61L24/0042C08J9/28A61L2400/04A61L2300/232A61L2300/102A61L2300/418C08J2201/0484C08J2305/08C08J2405/08C08J2405/02C08L5/08C08L5/02
Inventor 徐婷王荣
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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