Natural polysaccharide sponge and preparation method and application thereof
A natural polysaccharide and sponge technology, applied in the field of natural polysaccharide sponge and its preparation, can solve the problem of lack of sponge and achieve the effect of excellent biocompatibility and uniform dispersion
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[0051] (1) Preparation of aldehyde-based natural polysaccharide: 1 to 5 g of chondrogen sulfate, hyaluronate or dextran, pour in deionized water, stirred at room temperature until completely dissolved, resulting in a concentration of 1% (Wt / V) polysaccharide aqueous solution; Subsequis, addition of high-iodate, added according to the ratio of natural polysaccharide and sodium high -odeate ratio of 1 to 10: 1, and the protected reaction is 1 ~ 3h; then, excess ethylene glycol is added. After the reaction was stopped, stirring was continued for 1 to 2 h; -18 ° C is refrigerated.
[0052] (2) Hemolyulfurization rate test: Take the rabbit artery blood, add 20 ml of PBS buffer (pH 7.4) to 10 ml of rabbit whole blood, and gently mixed the mixture at 3000 rpm for 6 minutes. The red blood cells (RBC) were then collected from the bottom of the centrifuge tube, and then the same as described above was repeated with the ratio of the RBC and the PBS buffer (pH 7.4) volume ratio of 1: 2. Nex...
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[0062] Example 1
[0063] Preparation of natural polysaccharide sponge:
[0064] (1) The carboxymethyl chitosan and kaolin were dissolved in physiological saline to obtain solution A containing 2 wt.% CMCs and 0.5 wt.% KA;
[0065] (2) Preparation of a hyaluronic acid, wherein the mass ratio of sodium hyaluronate and sodium metatide is 1: 1, the expansion reaction time is 2 h, the dialysis bag is intercepted by 3500, aldehyde-based hyaluronic acid Dissolved in physiological saline, resulting in solution B containing 5 wt.% Aldehyde-hyaluronic acid.
[0066] (3) Comparative Solution A and solution B mixed with a volume ratio of 3: 7, and the gelation of the gelation of the gelation was obtained by hydrogel;
[0067] (4) The hydrogel was frozen at -80 ° C for 48 h, followed by freeze drying, and the natural polysaccharide sponge was obtained.
[0068] In the natural polysaccharide sponge, 14 parts of a carboxymethyl chitosan, 3.5 parts of kaolin, and 83 aldoxylated hyaluronic acid w...
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[0070] Example 2
[0071] Preparation of natural polysaccharide sponge:
[0072] (1) The carboxymethyl chitosan and kaolin were dissolved in physiological saline to obtain a solution A containing 4 wt.% CMCS and 2 wt.% KA;
[0073] (2) Posterosyl aldehyde sulfate is prepared, wherein the mass ratio of chondroitin and sodium sulfate is 3: 1, and the expansion reaction time is 1 h, and the dialysis bag is from 8,000 to 14,000, and aldeh-based sulfuric acid cartilage The dissolved in physiological saline, resulting in a solution B containing 4 wt.% Aldehyde sulfate chondroitin;
[0074] (3) The solution A and solution B are mixed with a volume ratio of 5: 5, and 125 s is sealed at room temperature to obtain a hydrogel;
[0075] (4) The hydrogel was frozen at -80 ° C for 72 h, followed by freeze drying treatment, and the natural polysaccharide sponge was obtained.
[0076] In the natural polysaccharide sponge, 40 parts by weight of carboxymethyl chitosan, 20 parts of kaolin, and 40 se...
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