Method for preparing high-purity herba epimedii essential oil and preparing anhydroicaritin by enzyme method

Abstract

The invention discloses a method for preparing high-purity herba epimedii essential oil and preparing anhydroicaritin by an enzyme method. According to the method, an ultrasonic technology is combined with a subcritical R134a fluid technology, a molecular distillation technology, a vacuum decompression boiling extraction technology, an enzyme immobilization technology, an enzyme one-pot catalysis technology, a macroporous resin purification technology, a recrystallization technology, a supercritical CO2 fluid drying technology and a full-automatic control technology, extraction, enzymolysis and purification are performed on the herba epimedii essential oil and herba epimedii total flavonoids under the synergistic effect of multiple raw materials, the herba epimedii oil and herba epimedii total flavonoids extracting solution are extracted continuously step by step in the same device, and the vacuum concentration is performed on the extracting solution to obtain herba epimedii total flavonoids extract, and the extract is converted from the crude product of the herba epimedii total flavonoids into a crude product of anhydroicaritin through enzymolysis. According to the method, the technology for producing the herba epimedii essential oil and the anhydroicaritin is high in resource utilization degree, high in product yield, high in product purity, low in residual solvent, low in energy consumption and small in environmental pollution and is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, enzymolysis and purification, and specifically relates to a method for preparing high-purity epimedium essential oil and enzymatically preparing icariin. Background technique [0002] Epimedium, alias: three-branch nine-leaf grass, fairy spleen, ox horn flower, three-pointed wind, sheep horn wind, triangular lotus, is a genus with about 50 species, likes damp areas, and generally grows in grass on hillsides and beside ditches , Under forests, in bushes and in crevices of rocks and rocks, it has not yet been artificially introduced and cultivated. Epimedium is a commonly used traditional Chinese medicine in China. It has high medicinal value, tonifies kidney and strengthens yang, and dispels wind and dampness. Treatment of impotence and impotence, hemiplegia, urination, urination, muscle and bone spasm, cough, blue blindness after illness, toothache due to deficiency fire, hemiplegia, we...

AI Technical Summary

Problems solved by technology

[0014] The traditional extraction method of Epimedium is mainly based on water decoction, but the above methods have defects in varying degrees: the water decoction has low efficiency, high energy consumption, and single extraction components; in recent years, ethanol-water solution has been used as the extraction method. Solvent, under the condition of microwave heating to extract dry Epimedium stem and leaf extraction technology

This method has the advantages of high reaction rate and high reaction conversion rate, but the technical reaction temperature is high, the enzyme cannot be reused, and it is difficult to produce on an industrial scale, resulting in high production costs.