The invention discloses NADH-containing (reduced nicotinamide adenine dinucleotide-containing) composition and preparation to improve climacteric symptoms and their preparation methods and applications and relates to the field of medicine and health. The composition includes, by weight, 1-10 parts of NADH, 1-10 parts of secondary anhydroicaritin, 1-10 parts of chondroitin sulfate, 1-10 parts of ursodesoxycholic acid, 1-10 parts of sialic acid, 1-10 parts of S-adenosyl methionine, 1-10 parts of polygala root, 1-10 parts of DHA (docosahexaenoic acid), and 1-10 parts of gamma-aminobutyric acid. The components of the composition are in suitable ratio and can coact to all-directionally improve climacteric symptoms safely with no side effects. The preparation method of the preparation has a simple process flow, short process time and zero byproducts, is low in production cost and is easy to apply to large-scale production.