Bacterium for inhibiting pathogenic bacteria of tobacco diseases and application

A technology of bacteria and tobacco, applied in the direction of application, bacteria, chemicals for biological control, etc., to achieve the effects of simple production process, good colonization effect, and wide sources

Active Publication Date: 2022-01-25
YUNNAN ACAD OF TOBACCO AGRI SCI
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Currently, there are no microbial report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterium for inhibiting pathogenic bacteria of tobacco diseases and application
  • Bacterium for inhibiting pathogenic bacteria of tobacco diseases and application
  • Bacterium for inhibiting pathogenic bacteria of tobacco diseases and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] - Acquisition of Bacillus velezensis WY2

[0132] A. Separation:

[0133] Take 0.4g of tobacco plant stem tissue that has been thoroughly sterilized on the surface, put it into a 2mL sterilized centrifuge tube together with sterilized steel balls, add 600μL of sterile water, and grind it with a tissue grinder for 3min at a frequency of 30r / s. Gradually dilute the ground tissue fluid to 10 -4 , 200 μL of homogenate was applied to the separation medium (5.0 g of peptone, 3.0 g of beef extract, 8.0 g of sodium chloride, 15.0 g of agar, pH 7.0) of each gradient, and each treatment was repeated 4 times. After culturing in the dark at 28°C for 48 hours, pick a single colony according to the colony shape, color, etc., re-stretch and purify on the separation medium, and transfer the single colony cultured by the streak to the slant of the separation medium test tube for storage;

[0134] B. Screening:

[0135] Using the plate confrontation method, use a hole puncher with a d...

Embodiment 2

[0144] ——Identification and preservation of Bacillus velezensis WY2

[0145] (1) Identification of Bacillus velezensis WY2

[0146]For the strain WY2 selected and bred above, biological analysis and molecular biological identification were carried out by conventional methods. The molecular identification method is as follows: Bacteria Genomic DNA Extraction Kit, see the kit instruction manual for the method. Primer F27 / R1492 was selected for PCR amplification, amplified under conventional conditions, the amplified product was recovered by gel, connected to the vector pEAZY-T5 Zero vector, heat-shock transformed Escherichia coli competent cell DH5α, and colonies were picked with M13F / M13R as primers Perform colony PCR identification. Positive clones were sent to Shanghai Sangon Biotechnology Co., Ltd. for sequence determination.

[0147] The test results are recorded as follows:

[0148] 1. Culture characteristics: The strain was cultured on NA plate medium at 28°C. In the ...

Embodiment 3

[0157] ——The control experiment of Bacillus velezensis WY2 on tobacco Fusarium root rot

[0158] The experiment was carried out in Chengjiang County, Yuxi City, Yunnan Province, and the tobacco variety was "Yunyan 87".

[0159] experiment method:

[0160] a. Fermentation: Activate the strain WY2 from a -80°C ultra-low temperature refrigerator in a slant medium (10.0g peptone, 3.0g beef extract, 5.0g sodium chloride, 15.0g agar, pH 7.0), and culture in a constant temperature incubator at 28°C 48h; Pick a ring of bacteria and inoculate it into a 50mL centrifuge tube containing 10mL of seed culture solution (10.0g of peptone, 3.0g of beef extract, 5.0g of sodium chloride, 15.0g of agar, pH 7.0), and place in a constant temperature incubator at 28°C Cultivate for 48 hours; then transfer the seed solution to 1000mL fermentation medium (peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, agar 15.0g, pH 7.0), and place in a constant temperature incubator at 28°C for 72 hours , ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bacterium. The bacterium is Bacillus velezensis WY2. The preservation place is Institute of Microbiology, Chinese Academy of Sciences, Dadn Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.6662. According to the bacillus velezensis WY2 disclosed by the invention, the effective control on the tobacco root rot can be realized, and the relative control effect is over 60 percent; and the bacterium also has a very good bacteriostatic effect on phytophthora parasitica, botrytis cinerea, sclerotinia sclerotiorum, target leaf pathogen, mildew pathogen, alternaria alternata and the like. The bacterium is good in prevention and treatment effect and has high application and popularization value.

Description

technical field [0001] The invention belongs to the technical field of bacteria for inhibiting tobacco disease pathogenic bacteria, including the field of acquisition method and application technology. Background technique [0002] Tobacco is one of the important economic crops in our country, and it is an important economic source of national finance and taxation, but the occurrence of diseases and insect pests seriously affects tobacco production. In recent years, with climate change and changes in farming patterns, the incidence of tobacco diseases has increased year by year. Among tobacco diseases, the most common and serious diseases are black shank, red spot and viral diseases, followed by bacterial wilt, wildfire, angular spot, root black rot and root-knot nematode. Only these 8 diseases The occurrence area of ​​the disease just accounts for 50% and above of the total tobacco planting area in the country, and accounts for more than 80% of the total output value loss ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P3/00C12R1/07
CPCC12N1/20C12N1/02A01N63/22
Inventor 盖晓彤卢灿华姜宁夏振远马俊红莫笑晗户艳霞王继明何元胜代快
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap