Method for leaching refractory uranium ore by bacteria
A uranium ore and bacteria technology, applied in the field of bacterial leaching of refractory uranium ore, can solve the problems of unsuitable for stable growth of leaching bacteria, lack of sulfur-oxidizing bacteria, distribution dynamics of leaching microbial community and difficulty in regulating leaching behavior, etc. Achieve the effect of increasing the uranium leaching rate and improving the adaptability of the flora
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Embodiment 1
[0041] Ore Handling and Preparation
[0042] The refractory uranium ore is crushed to a particle size of -5mm, and the ore sample is mixed and sampled by the heap cone quartering method, and the ore composition and phase are detected. The uranium ore sample comes from a uranium mine in Guangdong. Table 1 shows the particle size distribution of the experimental ore sample. A representative sample was taken to detect the composition of the ore sample. Ammonia titration is measured, and other elements are analyzed by X-ray fluorescence spectrometry (S4 Pioneer, Bruker, Germany). The results are shown in Table 2. The sulfur content is only 0.17%, which reflects the feasibility of sulfur strengthening; Mineral quantitative analysis system (MLA250, Czech Republic) analysis, the results show that the main uranium mineral in the ore sample is titanium uranium ore (UTi 2 o 6 ), uranite (U(SiO 4 ) 0.9 (OH) 0.4 ), and a small amount of pitchblende; gangue minerals mainly include alb...
Embodiment 2
[0049] Column immersion device
[0050] In order to evaluate the effect of sulfur enhancement on uranium leaching by bacterial flora in column leaching reactors, five sets of bacterial column leaching uranium experiments were designed with sulfur dosages of 0.5, 1, 2, and 4 g / kg ore (blank control without adding sulfur). Each column immersion system consists of a reaction column and an adsorption column. Schematic diagram of the biocolumn leaching reactor figure 1 shown.
[0051] The reaction column is made of a plexiglass tube with a thickness of 10 mm, a height of 220 cm, and an inner diameter of 10 cm. The adsorption column is 150cm high and 1cm in inner diameter, and is connected with each reaction column. They are used to adsorb uranyl ions in the leach solution. A layer of quartz sand with a diameter of 5-10mm was placed as a support rock at the bottom of each column to a depth of 50mm. Take 25kg of ore samples after the heap cone quartering method, and add 0.5, 1, ...
Embodiment 3
[0053] Leaching method of uranium ore
[0054] 1. Acid presoak
[0055] The purpose of acid pre-leaching is to acidify alkaline or neutral minerals and create a good environment for subsequent bioleaching.
[0056] First, use a sulfuric acid solution with a pH of about 0.6 (dilute the sulfuric acid to 12.3g / L) for acidification treatment, add an appropriate amount of sulfuric acid to the adsorption tail liquid to prepare a immersion solution, and spray for 10 days; then use a pH of 1-2 The dilute sulfuric acid was sprayed for about 10 days, and when the pH of the adsorption tail liquid was stable at about 3.0, the acid leaching was terminated.
[0057] 2. Activation, acclimatization and expansion of strains
[0058] The activation of bacterial strains A.ferrooxidans ATCC 23270 and L.ferriphilum YSK adopts adding 44.7g / L ferrous sulfate (FeSO 4 ·7H 2 O) 9K culture medium activation culture, A.thiooxidans A01 adopts the 9K medium activation culture that adds 10g / L sulfur pow...
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