Method for extracting dihydromyricetin from ampelopsis grossedentata

A technology of dihydromyricetin and rattan tea, applied in the direction of organic chemistry, etc., can solve the problems of flammability, no report on the extraction and preparation of dihydromyricetin, and environmental protection, and achieve the effect of easy preparation, low raw material price, and non-volatile effect

Active Publication Date: 2022-02-11
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional extraction methods of dihydromyricetin mainly include organic solvent extraction, alkaline extraction, enzyme extraction and water extraction, among which organic solvent extraction is currently the most commonly used, however, organic solvents have many disadvantages, such as not environmentally friendly, flammable , restrict the use of certain foods, etc.
Although there are many reports on the application of deep eutectic solvents in the extraction of natural active substances, there is no report on the extraction and preparation of dihydromyricetin, which is rich in vine tea

Method used

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  • Method for extracting dihydromyricetin from ampelopsis grossedentata
  • Method for extracting dihydromyricetin from ampelopsis grossedentata
  • Method for extracting dihydromyricetin from ampelopsis grossedentata

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: the selection of DES and the selection of DES extraction condition

[0031] (1) Preparation of deep eutectic solvent: mix the hydrogen bond acceptor and hydrogen bond donor uniformly in a certain molar ratio. The specific hydrogen bond acceptor and hydrogen bond donor and their molar ratio are shown in Table 1, and put into a sealed Shake in a glass bottle at 200 rpm at 80°C for 3 hours to obtain a pure and uniform liquid, and then stand at 30°C overnight. In this implementation, a total of 32 deep eutectic solvents were prepared for testing.

[0032] (2) Deep eutectic solvent extraction: the solution obtained in (1) and water are made into 30% (volume ratio) extraction solvent A, and the vine tea powder and extraction solvent A are mixed evenly at a material-to-liquid ratio of 1:20g / mL , at 45°C, shaking in a shaking table with a rotating speed of 200rpm for 2h to obtain the extract of dihydromyricetin, and the yield of dihydromyricetin in the extract wa...

Embodiment 2

[0052] Embodiment 2: the selection of macroporous resin and the selection of loading conditions

[0053] Macroporous resin separation: get No. 12 DES in the embodiment 1 as the dihydromyricetin extract 1mL that extractant obtains, load sample on macroporous resin successively with a large amount of distilled water, 80% ethanol elution (10mL), load sample After the ethanol elution part was collected to obtain eluate A, the eluate was concentrated to obtain concentrate B, and the yield of purity was determined.

[0054] (a) Screening of macroporous resin

[0055] Choose XAD1600N, XAD16, XAD1180, XAD7HP, D101, S-8, AB-8, DM301, X-5 nine kinds of macroporous resins, carry out the adsorption recovery experiment of dihydromyricetin extract, the result is AB-8, DM301 or XAD -7HP has a better effect on dihydromyricetin ( Figure 5 a). DM301 was reused 6 times, and the recovery rate was good ( Figure 5 b).

[0056] (b) Study on the influence of loading conditions

[0057] Specif...

Embodiment 3

[0058] Embodiment 3: A kind of method extracting dihydromyricetin from rattan tea

[0059] 1) Preparation of DES: eutectic hydrogen bond acceptor tetrabutylammonium bromide and hydrogen bond donor pyruvate in a molar ratio of 1:2, the eutectic condition is: shaking at 200rpm in a shaker at 80°C for 3.5 hours to obtain DES , let stand overnight;

[0060] 2) DES extraction: the DES obtained in step 1) is mixed with water to form an extraction solvent with a concentration of 70%, then the vine tea powder and the extraction solvent are mixed evenly at a liquid-to-material ratio of 20mL / g, and the particle diameter of the vine tea powder is less than 1mm, the water content of rattan tea powder is 11%, 45 ℃, shaking in a shaker with a rotating speed of 200rpm for 2.5h, to obtain the extract of dihydromyricetin; the yield of dihydromyricetin in the extract is detected by HPLC to reach 40.09 %;

[0061] 3) Adsorption and separation of macroporous resin: take 1ml of the extract of di...

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Abstract

The invention discloses a method for extracting dihydromyricetin from ampelopsis grossedentata, which specifically comprises the steps of 1) co-melting a hydrogen bond acceptor and a hydrogen bond donor to obtain DES, and standing overnight; 2) preparing DES and water into an extraction solvent, then uniformly mixing the ampelopsis grossedentata powder with the extraction solvent, and performing vibration extraction to obtain an extracting solution of dihydromyricetin; and (3) sampling the extracting solution of dihydromyricetin on macroporous resin, eluting by using distilled water, 80% ethanol and 95% ethanol in sequence, collecting to obtain an 80% ethanol eluent A, and concentrating the eluent A to obtain a concentrate B, namely, the dihydromyricetin monomer component. According to the invention, the dihydromyricetin compound can be simply, conveniently and efficiently obtained from the ampelopsis grossedentata, the yield of the extracted dihydromyricetin is high and is 10-20% higher than that of a traditional organic solvent, and the method has the advantages that the extraction procedure is simple and safe, the extraction efficiency is high, the analysis time is short, no by-product is generated in the extraction process, and the method has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction and separation, and in particular relates to a method for obtaining dihydromyricetin from rattan tea by using a deep eutectic solvent combined with macroporous resin chromatography column technology. Background technique [0002] Vine tea is a wild woody deciduous vine of the grape family Vitaceae michx Ampelopsis grossedentata (Hand-Mazz) W.T. It is native to various parts of the Jiangnan mountainous area in China. It has been used as a tea drink in the folk for hundreds of years. In 2013, it was approved as a new resource food raw material by the Health and Family Planning Commission. Studies in recent years have shown that vine tea is rich in flavonoid functional active ingredients, including dihydromyricetin, myricetin, myricetin, etc., and the content of dihydromyricetin in vine tea can be as high as 30–40%; it has anti-inflammatory properties. Tumor, anti-inflammatory, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 王丽玲黄旭波秦玉川王衍彬方茹童晓青
Owner ZHEJIANG FORESTRY ACAD
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