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Saccharomyces cerevisiae SWGCJM001 as well as culture method and application thereof

A technology of Saccharomyces cerevisiae and its cultivation method, which is applied in the field of Saccharomyces cerevisiae SWGCJM001 and its cultivation, can solve the problems of low xylose purity, high reaction pressure, and inability to meet industrial production, and achieve economic benefits, mild reaction conditions, and good industrial production. The effect of applying the foreground

Active Publication Date: 2022-03-04
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1955, Courtois, J.E. reported that 2,4-O-benzylene-D-sorbitol was used as a raw material to synthesize L-xylose by a two-step method of first oxidation and then hydrolysis, but the catalytic oxidant used in this method was extremely unstable. Especially easy to decompose, the reaction process is difficult to control
In 2003, it was reported in U.S. Patent 20030097029 that L-xylose was hydrogenated and reduced under the action of a nail catalyst to generate L-xylose. The reaction pressure was 5 MPa and the reaction time was 18 hours. The reaction pressure was high and the reaction time was long. Under this condition, L -Xylose will continue to be excessively reduced to form xylitol, and the purity of the resulting xylose is not high
In 2017, Chinese patent 108276455A reported the use of 2,4-benzene-L-xylose as a substrate to synthesize L-xylose under the action of an acid catalyst, with a product yield of about 80% and a product purity of 98% , more by-products are produced, and the purification is difficult. At this stage, it is still limited to laboratory research and cannot meet the requirements of industrial production.
[0004] Therefore provide a kind of synthesis method of L-xylose, solve the synthesis process pressure of L-xylose in the prior art too high, the reaction time is long, the reaction process control is difficult, post-processing is loaded down with trivial details, the problem that is difficult for industrialization has become the technology in the art Problems that people need to solve

Method used

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  • Saccharomyces cerevisiae SWGCJM001 as well as culture method and application thereof
  • Saccharomyces cerevisiae SWGCJM001 as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Isolation, Screening and Identification of Saccharomyces cerevisiae SWGCJM001

[0048] The soil samples randomly collected from the farmland were diluted with sterile water at 1:100 (W / V), and spread on the screening plate medium. The screening plate medium (pH 7.0) included: 1% potassium dihydrogen phosphate, 0.3 % diammonium hydrogen phosphate, 0.5% yeast extract, 1% tryptone, 5% xylitol, 15% agar. Cultivate at 30°C for 48h, then select a single colony and inoculate them into the liquid selection medium, and culture at 30°C and 200rpm for 48h. After the culture was centrifuged at 8000rpm for 10min, the cells were added to the reaction liquid for reaction. The reaction liquid included 5% xylitol, pH 7.0 and 50mM potassium phosphate buffer, and reacted at 250rpm at 30°C for 24h. After the reaction liquid was centrifuged at 12000 rpm, it was detected by liquid phase high performance chromatography (HPLC).

[0049] The HPLC detection conditions are: column: Aminex HPX-8...

Embodiment 2

[0053] Fermentation of Saccharomyces cerevisiae SWGCJM001

[0054] Seed medium: 5% glucose, 0.5% yeast extract, 0.2% (NH 4 ) 2 SO 4 , 0.2% KH 2 PO 4 , 0.1% MgSO 4 ·7H 2 O, the pH value is 5.5; fermentation medium: 15% glucose, 0.2% yeast extract, 0.2% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 , the solvent is distilled water, and the pH value is adjusted to 5.5. The seed medium was sterilized at 115° C. for 25 minutes, inoculated after cooling, and cultured in shake flasks with a liquid volume of 30%.

[0055] Specific cultivation steps: take the bacteria stored at -70°C and draw a line on the seed solid plate, pick a single colony and inoculate it in the seed liquid medium, cultivate it at 30°C and 250rpm for 12 hours, and inoculate the seeds according to the inoculation amount of 5%. Inoculated in the fermentation medium, 30°C, 250rpm shaking culture for 24 hours, after the cultivation, the fermentation broth was centrifuged and washed twice with saline, and the wet bacter...

Embodiment 3

[0057] Production of L-xylose

[0058] In a 10L catalytic system, add 600g of xylitol, use pH7.0 phosphate buffer solution as the buffer, stir, add 10g of Saccharomyces cerevisiae SWGCJM001 strain, and start the reaction. After the reaction at 35° C. for 12 hours, the concentration of L-xylose in the solution can reach 44.79 g / L as detected by liquid chromatography, and the conversion rate can reach more than 96.54%. After the reaction, the reaction liquid was treated by membrane filtration, separated and purified, concentrated and crystallized, centrifuged and dried to obtain 427.72 g of finished product. After testing, the chiral purity of the finished L-xylose is 100%, the product purity is 99.90%, and the product yield is 95.5%.

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Abstract

The invention provides saccharomyces cerevisiae SWGCJM001 for synthesizing L-xylose through biological catalysis as well as a culture method and application of the saccharomyces cerevisiae SWGCJM001, and belongs to the technical field of functional bacterium development. The preservation number of the saccharomyces cerevisiae SWGCJM001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 20832. The strain is a new strain capable of catalytically synthesizing L-xylose by taking xylitol as a substrate, high-purity preparation of L-xylose can be realized through the strain, the conversion rate is 100%, and the optical purity reaches 100%. When the strain is used for an asymmetric reduction process, the reaction conditions are mild, energy is saved, the environment is protected, more importantly, the economic benefit of the L-xylose is remarkably improved, and the strain has a very good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of functional bacteria development, and in particular relates to a strain of Saccharomyces cerevisiae SWGCJM001 and its cultivation method and application. Background technique [0002] Xylose is a five-carbon sugar with two configurations, D- and L-. D-xylose mainly exists in plants and animals, L-xylose does not exist in nature, and L-xylose is widely used in medicine, food, chemical industry and other fields. As a pharmaceutical intermediate, L-xylose plays an important role in anti-cancer, anti-virus, anti-inflammation, anti-diabetes, etc. In terms of food, L-xylose can improve the microbial environment of the human body and improve the body's immunity. It is an ideal sweetener for humans, and it can also be used as a raw material for the synthesis of a healthy sweetener xylitol. [0003] Xylose is not a naturally occurring compound, only synthesized by chemical synthesis and biotransformation. The ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P19/02C12Q1/6895C12R1/865
CPCC12N1/18C12P19/02C12Q1/6895
Inventor 贾振华李冉宋聪宋水山张翔
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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