Nanometer sensing system based on CdTe-Ce quantum dot fluorescence and application of nanometer sensing system in levofloxacin detection
A technology of levofloxacin and a sensing system, which is applied in the field of nano-sensing systems, can solve the problems of large reagent flow consumption, weak accuracy, long liquid chromatography analysis time, etc., and achieves the promotion of development and application, low cost and high sensitivity. and selective effects
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Embodiment 1
[0017] Preparation of CdTe QDs
[0018] Synthesis of CdTe QDs: Cd(CH 3 CO 2 ) 2 2H 2 O and 3-mercaptopropionic acid were dissolved in deionized water, the pH was adjusted to slightly alkaline with NaOH aqueous solution, sonicated, and K 2 TeO 3 Stir with deionized water, then add NaBH 4 , ultrasonic treatment, the resulting mixture is heated to boiling and then reflux reaction, after the reaction, the obtained CdTe QDs are dried, the dry powder is collected, and stored in the refrigerator for later use;
[0019] Fluorescent detection of LVFX
[0020] The container used for the measurement was thoroughly washed several times with ethanol and dried in air. Then CdTe QDs and Ce 3+ At the same time, add to the system containing levofloxacin, collect the fluorescence signal immediately, and the fluorescence is recorded under the same conditions;
Embodiment 2
[0022] 1. Characterization of components in ratiometric fluorescence system
[0023] Considering that the detection sensitivity of the probe to the analyte is related to its own properties, FT-IR, UV-vis and fluorescence spectroscopy were used to study its structural features and spectral properties, respectively.
[0024] 2. The response of each component of the fluorescent system to LVFX
[0025] First, record the CdTe and Ce fluorescence intensities. Of course, we also discussed the optimal concentration. The relationship between the fluorescence intensity and the concentration of levofloxacin was studied, and a calibration curve was obtained. With the increase of levofloxacin concentration, the enhanced fluorescence of Ce gradually decreased, and the fluorescence of LVFX gradually increased. When the concentration of levofloxacin reaches 5.4*10-6M, the relationship between the signal and the concentration of levofloxacin conforms to the linear regression equation, and t...
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