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Preparation method of medical hydrogel, hydrogel and dressing

A hydrogel, medical technology, applied in medical science, bandages, etc., can solve the problems of reducing the blood coagulation mechanism of wounds, reducing the anti-inflammatory ability of drug-loaded hydrogels, and long time, so as to reduce the polarization of induced macrophages time, avoid the reduction of wound coagulation mechanism, and ensure the effect of anti-inflammatory effect

Active Publication Date: 2022-04-05
NANJING TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this type of drug-loaded hydrogel, heparin is more doped in the form of small pharmaceutical molecules, so the overall anti-inflammatory ability of the material will be limited by the encapsulation rate, drug loading and release rate of the hydrogel itself. , and the release of heparin is based on a certain carrier, and does not realize the direct contact between anti-inflammatory drugs and inflammatory factors, which will reduce the anti-inflammatory ability of the drug-loaded hydrogel, and for the repair of slow healing wounds such as diabetes, if the wound Using a large amount of heparin sodium on the surface will reduce the blood coagulation mechanism of the wound, causing the wound to bleed for a long time and cause other diseases
High-molecular-weight hyaluronic acid and mannose are common substances. They are widely used in the field of biomedical polymers due to their good biocompatibility. In recent years, studies have shown that they have a good effect on inducing the polarization of macrophages. , but it cannot be widely used because it takes a long time to induce

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  • Preparation method of medical hydrogel, hydrogel and dressing
  • Preparation method of medical hydrogel, hydrogel and dressing
  • Preparation method of medical hydrogel, hydrogel and dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Preparation of LA-HEP precursor

[0085] Prepare a 25mL reaction bottle, wash it, and pack it with tin foil. Add 0.5 g of lipoic acid to the reaction flask, vacuumize, and protect with nitrogen gas. Measure 3.9g of CDI and 2.5mL of DMAC, inject it into the reaction bottle with a needle, stir, and react at room temperature for 30min.

[0086] Dissolve 0.65g of HEP into 30mL of formamide, and heat to dissolve in an oil bath at 90°C. After the dissolution is complete, cool the reaction flask to room temperature. The product of the first step reaction was added to HEP, 0.015g DMAP was added and the reaction was continuously stirred for 4h, and the crude product was subjected to KH 2 PO 4 Neutralization, purification by dialysis, recovery by freeze drying.

[0087]Synthesis of MA-HHA

[0088] Weigh 0.5000g sodium hyaluronate and dissolve it in 100mL deionized water (0.5%), add 0.0312g 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride EDC.HCl, weigh 0.0220 g of...

Embodiment 2

[0095] Preparation of LA-HEP precursor

[0096] Prepare a 25mL reaction bottle, wash it, and pack it with tin foil. Add 0.5 g of lipoic acid to the reaction flask, vacuumize, and protect with nitrogen gas. Measure 0.39g of CDI and 2.5mL of DMAC, inject it into the reaction bottle with a needle, stir, and react at room temperature for 60min.

[0097] Dissolve 0.65g of HEP into 30mL of formamide, and heat to dissolve in an oil bath at 90°C. After the dissolution is complete, cool the reaction flask to room temperature. The product of the first step reaction was added to HEP, 0.015g DMAP was added and the reaction was continuously stirred for 6h, and the crude product was subjected to KH 2 PO 4 Neutralization, purification by dialysis, recovery by freeze drying.

[0098] Synthesis of MA-HHA

[0099] Weigh 0.5000g sodium hyaluronate and dissolve it in 100mL deionized water (0.5%), add 0.0312g 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride EDC.HCl, weigh 0.0220 g ...

Embodiment 3

[0106] Preparation of LA-HEP precursor

[0107] Prepare a 25mL reaction bottle, wash it, and pack it with tin foil. Add 0.5 g of lipoic acid to the reaction flask, vacuumize, and protect with nitrogen gas. Measure 1.56g of CDI and 2.5mL of DMAC, inject it into the reaction bottle with a needle, stir, and react at room temperature for 30min.

[0108] Dissolve 0.65g of HEP into 30mL of formamide, and heat to dissolve in an oil bath at 90°C. After the dissolution is complete, cool the reaction flask to room temperature. The product of the first step reaction was added to HEP, 0.015g DMAP was added and the reaction was continuously stirred for 4h, and the crude product was subjected to KH 2 PO 4 Neutralization, purification by dialysis, recovery by freeze drying.

[0109] Synthesis of MA-HHA

[0110] Weigh 0.5000g sodium hyaluronate and dissolve it in 100mL deionized water (0.5%), add 0.0312g 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride EDC.HCl, weigh 0.0220 g ...

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Abstract

The invention provides a method for preparing medical hydrogel, and the preparation method comprises the following steps: respectively modifying LA onto HEP and MA-HHA in an esterification manner to prepare precursors of the hydrogel, namely LA-HEP and LA-MA-HHA. And adding a catalytic amount of a reducing agent into the precursor mixed solution, triggering the disulfide bond part in the lipoic acid to be broken to form sulfydryl, and forming a disulfide bond again through sulfydryl-disulfide bond dynamic exchange reaction to initiate crosslinking of the two precursors to form the hydrogel. The invention also provides a medical hydrogel and a dressing comprising the hydrogel. According to the invention, heparin sodium, lipoic acid, hyaluronic acid and mannose are used as raw materials of a composite material, and the hydrogel is prepared by adopting an active controllable crosslinking strategy of a sulfydryl-disulfide bond dynamic exchange reaction, and has the effects of continuously resisting inflammation and promoting repair in batches.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing a medical hydrogel and a medical dressing containing the hydrogel. Background technique [0002] Diabetic food syndrome is one of the major health problems of people with diabetes worldwide. Diabetes is a chronic hyperglycemia that is generally divided into two prevalent types, namely type 1 diabetes and type 2 diabetes (T2D), where T2D is Non-insulin-dependent diabetes caused by autoimmune insulin deficiency and insulin resistance, while 90-95% of diabetic patients have T2D. Wound healing is a complex and highly regulated process, which is the key to maintaining the skin barrier function, and the wounds of diabetic patients have slow healing or even non-healing, which leads to diabetic foot and amputation, which is the reason for the high disability rate of diabetes Major complications, in severe cases, can threaten the patient's life. The healing o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L5/10C08L5/08A61L26/00
Inventor 何一燕宋吉亮苑明秦加骏姜于航彭万佳顾忠伟
Owner NANJING TECH UNIV