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Bio-control inoculant for inhibiting streptococcus agalactiae as well as preparation method and application of bio-control inoculant

A biocontrol agent, Streptococcus lactis technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc. The effect of inhibiting growth and excellent bactericidal effect

Pending Publication Date: 2022-04-15
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current research shows that resistance to clindamycin, erythromycin, and fluoroquinolones in GBS is on the rise, and resistance to vancomycin has also been reported, which makes patients allergic to penicillins or cephalosporins There is a big problem with the treatment

Method used

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  • Bio-control inoculant for inhibiting streptococcus agalactiae as well as preparation method and application of bio-control inoculant
  • Bio-control inoculant for inhibiting streptococcus agalactiae as well as preparation method and application of bio-control inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The bacteriostasis effect of embodiment 1-boror-resistant lysine bacillus fermented liquid

[0036] The inhibitory effect of boron-resistant Bacillus lysinica on the growth of Streptococcus agalactiae was determined by the method of toxic medium.

[0037] Experimental group 1:

[0038] Experimental group 1-1:

[0039] The seed culture solution includes the following components: 5g / L yeast extract, 10g / L tryptone and 10g / L sodium chloride, pH 7.0;

[0040] Proliferation medium includes the following components: 5.2g / L yeast extract, 10.5g / L tryptone, 10.5g / L sodium chloride and 2.8g / L polyglutamic acid, pH 7.0;

[0041] The fermentation broth includes the following components: 5.2g / L yeast extract, 10.5g / L tryptone, 10.5g / L sodium chloride, 1.2g / L polyglutamic acid and 0.3g / L petracenin, pH The value is 6.8; according to the distribution of the above components, the seed culture solution, proliferation culture solution and fermentation culture solution are prepared, s...

Embodiment 2

[0073] The control experiment of embodiment 2-tilapia streptococcosis

[0074] A biocontrol bacterium agent for inhibiting Streptococcus agalactiae, consisting of 30 parts of boron-resistant lysine bacillus fermentation liquid prepared by experimental group 1-1, 10 parts of water and 14 parts of corn gluten powder;

[0075] The preparation method of the biocontrol fungus is as follows: mixing the fermented liquid of boron-resistant lysine bacillus and water, adding kaolin, stirring, spraying and drying, and pulverizing to obtain the biocontrol fungus;

[0076] Select 40 healthy tilapias with a weight of 80-90g, divide them into 2 groups, 20 fishes in each group, feed tilapia feed twice a day on time, change the water every 5 days, adjust the water quality, and remove the bio-control bacteria. 1:200 mixture of florfenicol and tilapia feed was used as the test group, and florfenicol was mixed with tilapia feed 1:2000 as the drug group; after feeding for 10 days, 0.2 mL dose of ...

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Abstract

The invention provides a biocontrol inoculant for inhibiting streptococcus agalactiae, which comprises the following components in parts by weight: 20-40 parts of boron-resistant lysine bacillus fermentation liquor and 8-12 parts of an auxiliary material which is at least one of water, a culture medium and glycerol, the boron-resistant lysine bacillus fermentation liquor is obtained by carrying out secondary fermentation culture on boron-resistant lysine bacillus. The biocontrol inoculant for inhibiting streptococcus agalactiae can effectively inhibit the growth of streptococcus agalactiae, the inhibition rate of fermentation liquor within 36 hours can reach 49.5% or above, and the biocontrol inoculant can be used for preventing or treating infectious diseases caused by streptococcus agalactiae, and especially can effectively prevent or treat streptococcicosis of tilapia infected by streptococcus agalactiae. The boron-resistant lysine bacillus not only can reduce infection of streptococcus agalactiae to a great extent, but also has no influence on the ecological environment, is expected to be used as a high-quality chassis microorganism for synthetic biological research, and brings a new direction for prevention and treatment of streptococcus agalactiae.

Description

technical field [0001] The invention relates to the technical field of application of biocontrol bacteria, in particular to a biocontrol agent for inhibiting Streptococcus agalactiae and its preparation method and application. Background technique [0002] Streptococcus agalactiae, also known as group B streptococcus (GBS), is an opportunistic pathogen with strong pathogenicity to humans. The main infected population is the elderly, people with weakened immunity, especially newborns. Streptococcus agalactiae is very easy to colonize the vagina of pregnant women, causing intrauterine infection. It is the main cause of neonatal sepsis, pneumonia, meningitis and endocarditis, and has a high mortality rate. At the same time, Streptococcus agalactiae is also the main pathogen causing streptococcosis in tilapia. Current treatments are as follows: [0003] Antibiotic therapy is currently the main treatment. Strategies to prevent neonatal and maternal GBS disease include antenat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/01
Inventor 李娟娟刘柱王丹陈银华章跃陵林向民
Owner HAINAN UNIVERSITY
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