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Application of Ermiao pill in preparation of medicine for treating or preventing periodontitis

A periodontitis and ermiao pill technology, applied in the field of medicine, can solve the problems of antibiotic drug resistance, long treatment period, toxic effects of anti-inflammatory drugs, etc.

Pending Publication Date: 2022-04-26
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-surgical treatment to remove the irritation of local periodontal plaque and calculus, including supragingival scaling, subgingival scaling, root planing, supplemented with systemic or local antibiotics, non-steroidal anti-inflammatory drugs Inflammation therapy, immunomodulatory drug therapy, and drug therapy supplemented by photodynamic therapy, laser, probiotic therapy, etc., but these treatments have certain limitations, such as the removal of dental plaque and calculus, there are anatomical structures In difficult-to-reach areas, antibiotic drug treatment has drug resistance, and anti-inflammatory drugs have toxic effects; and the treatment cycle is long, time-consuming and costly, and patients are unfavorable

Method used

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  • Application of Ermiao pill in preparation of medicine for treating or preventing periodontitis
  • Application of Ermiao pill in preparation of medicine for treating or preventing periodontitis
  • Application of Ermiao pill in preparation of medicine for treating or preventing periodontitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 2

[0042] The extract solution preparation of embodiment 1 Ermiao pills

[0043] 1.1. Experimental materials

[0044]Cangzhu 250g, Phellodendron Phellodendri (fried with salt) 250g, purchased from Shanghai Kangqiao Chinese Herbal Pieces Co., Ltd., identified by the inventor according to the 2020 edition of the Chinese Pharmacopoeia.

[0045] 1.2. Experimental method

[0046] Atractylodes atractylodis 250g, Phellodendron Phellodendri (fried with salt) 250g, Erwei medicinal materials were extracted by boiling with 10 times the volume of water for 1 hour, filtered, and the residue was continued to be extracted by boiling with 10 times the volume of water for 1 hour (repeat twice), and the three times the filtrate was evaporated on a rotary evaporator After being concentrated in the instrument, it was prepared into a 1g / mL Ermiao pill solution, which was stored in the refrigerator for later use.

Embodiment 2

[0048] 2.1. Experimental materials

[0049] Chloral hydrate, 1% iodophor (Adamas);

[0050] 1% povidone iodine (Shandong Lierkang Medical Technology Co., Ltd.);

[0051] C57BL / 6 male mice (Tongji University Experimental Animal Center);

[0052] 5-0 silk thread (JOSON Johnson);

[0053] 2.2. Experimental steps

[0054] Twenty-four 8-week-old SPF grade C57BL / 6 male mice, weighing 20-30 g, were provided by the Experimental Animal Center of Tongji University. All mice were kept in an environment of 22°C room temperature, 50% humidity, 12h light, and no pathogenic bacteria. Breeding, the room was kept well ventilated, and the mice were free to drink water and eat food. After one week of feeding, they were used for model preparation.

[0055] The mice were randomly divided into 6 groups, respectively blank group (Control), periodontitis model group (P), model group given Ermiao Pill group (EMW), positive control drug (doxycycline group 20mg / kg d )(DOX).

[0056] Periodontitis ...

Embodiment 3

[0059] The mouse maxillary bone tissue of Example 2 was taken for fixation, dehydration, embedding, sectioning, and HE staining analysis.

[0060] experimental method:

[0061] 3.1. The maxilla was fixed in 4% PFA for 48 hours, and decalcified in 10% EDTA decalcifying agent for about 3 months. The paraffin-embedded samples were cut into 5 μm serial sections, running through the distal root of M1 and the mesial root of M2, ensuring that the observed positions of each sample were exactly the same for subsequent histological staining;

[0062] 3.2. Mark the slices with the name, location, and batch, and put them in a 65°C oven for more than 1 hour;

[0063] 3.3. The slices were soaked in xylene Ⅰ for 15 minutes, xylene Ⅱ for 15 minutes; 100% ethanol Ⅱ for 3 minutes; 100% ethanol Ⅰ for 3 minutes; 95% ethanol for 3 minutes; 80% ethanol for 3 minutes; 70% ethanol for 3 minutes; 50% ethanol for 3 minutes. 3min; distilled water, 5min; hematoxylin, 2min; tap water, 4min; eosin, 5sec-...

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Abstract

After the Ermiao pill is given to a periodontitis mouse, damage to periodontium can be effectively inhibited, the Ermiao pill has an obvious protection effect on alveolar bone, alveolar bone absorption is inhibited, inflammatory response can be relieved, and periodontitis can be treated; therefore, the Ermiao pill can be used as a medicine for treating and preventing periodontitis, the problem that periodontitis cannot be radically treated by adopting a medicine in the prior art is solved, a new choice is provided for treating periodontitis, and compared with an existing treatment method, the treatment method is simpler, small in toxic and side effects, safe, economical, convenient, good in patient compliance and wide in popularization crowd.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Ermiao Pills in the preparation of medicines for treating or preventing periodontitis. Background technique [0002] Periodontitis is a common disease in the Department of Stomatology, which refers to the destruction of periodontal supporting tissues (periodontal ligament, alveolar bone and cementum) - the formation of periodontal pockets - resulting in inflammation, the course of the disease is long, and it often occurs in the adult population. It is characterized by loss of attachment, formation of periodontal pockets, and alveolar bone resorption, followed by loosening of teeth, eventual tooth loss, loss of masticatory function, impaired pronunciation, and affected appearance, seriously affecting work and life. It has been determined by the World Health Organization as the third killer that threatens human health after malignant tumors and cardiovascular an...

Claims

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Application Information

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IPC IPC(8): A61K36/756A61P1/02A61K36/284
CPCA61K36/284A61K36/756A61P1/02A61K2236/331A61K2236/39A61K2300/00
Inventor 夏正祥
Owner TONGJI UNIV
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