SARS-CoV-2 yolk neutralizing antibody IgY spray for preventing and treating new coronal pneumonia

A sars-cov-2, spray technology, applied in the field of epidemic prevention, can solve the problems of increased virus fatality rate, less plasma source, and difficult to meet the treatment of large patients

Active Publication Date: 2022-05-13
上海博满生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the plasma of recovered patients in the recovery period of patients infected with the new coronavirus also contains neutralizing antibodies to the new coronavirus, which can treat patients infected with the new coronavirus, but because the plasma may contain other pathogens, it will cause other infectious diseases, and because Rehabilitation patients have few sources of plasma, and it is difficult to meet the treatment needs of a large number of patients
[0007] Many scientists have turned to the development of humanized neutralizing antibody IgG for the new coronavirus, but humanized neutralizing antibody IgG sometimes produces "antibody-dependent enhancement (ADE)" after binding to the virus, assisting the virus to enter the target cell and increasing the infection rate , resulting in an increase of tens or even hundreds of times in the virus fatality rate

Method used

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  • SARS-CoV-2 yolk neutralizing antibody IgY spray for preventing and treating new coronal pneumonia
  • SARS-CoV-2 yolk neutralizing antibody IgY spray for preventing and treating new coronal pneumonia

Examples

Experimental program
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Embodiment 1

[0039] Example 1 Preparation of yolk neutralizing antibody IgY against novel coronavirus

[0040] 1. Immune laying hens with the RBD protein of the new coronavirus

[0041] Use the new coronavirus RBD protein (see SEQ ID NO.1 for its amino acid sequence) as the antigen, take 200 μg of the antigen each time, add an adjuvant suitable for immunizing chickens, antigen:adjuvant ISA 70=30:70, emulsification, volume ratio, Intramuscular injection of 0.5ml was carried out to laying hens, immunized 3 times, immunized at 3 points, and the interval between each time was 28 days.

[0042] 2. Extract and purify IgY antibody

[0043] Use egg yolk sieves to separate egg yolks from immune hens, add 9 times distilled water and stir to prepare egg yolk liquid, adjust pH to 5.2 with 1MolHCl, set aside for 5 hours at 4°C, centrifuge at 8000rpm for 20 minutes, discard the precipitate, and collect the supernatant. Slowly add n-octanoic acid dropwise to 1% to remove impurity protein and lecithin i...

Embodiment 2

[0056] The preparation of embodiment 2 new coronavirus egg yolk neutralizing antibody IgY spray:

[0057] The ingredients are proportioned according to the following percentages by weight: yolk neutralizing antibody IgY 0.3%, glycerin 10%, NISIN 0.02%, lysozyme 0.5%, chlorhexidine 0.02%, add deionized water to make up 100ml, Use Pasteur disinfection method to inactivate the virus, use 0.22μm membrane filter to sterilize, put the solution into a small portable spray tank and spray it for nasal spray, oral spray prevention, atomization emergency treatment or Emergency injection therapy.

Embodiment 3

[0058] Example 3 Preparation of Egg Yolk Neutralizing Antibody IgY Eye Drops for Prevention of New Coronary Pneumonia

[0059] The ingredients are proportioned according to the following weight percentages: yolk neutralizing antibody IgY of Example 1 0.3%, lysozyme 0.5%, NISIN 0.02%, EDTA-2Na 0.05%, NaCl 0.034%, benzalkonium 0.02%, Glycerin 5%, sorbitol 5%, water-soluble peppermint flavor 0.025%, disodium hydrogen phosphate 0.786%, sodium dihydrogen phosphate 1.04%, deionized water to make up the balance.

[0060] Viruses were inactivated by pasteurization and sterilized by filtration through a 0.22 μm membrane. Put the solution into a small carry-on eye drop bottle for eye drops.

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Abstract

According to the SARS-CoV-2 yolk neutralizing antibody IgY spray for preventing and treating the new coronal pneumonia, the yolk neutralizing antibody IgY is a polyclonal antibody, comprises a new coronal virus S1 protein receptor binding domain antibody or a new coronal virus S2 protein antibody, and can block the binding of a new coronal virus and a cell receptor ACE2, neutralize the new coronal virus SARS-CoV-2, block the infection of the new coronal virus and inhibit and kill the virus; new crown pneumonia is prevented and treated; the S protein, the S1 protein, the RBD protein or the S2 protein of the new coronavirus is used as an antigen to immunize an egg laying hen, and the egg yolk antibody IgY is extracted from egg yolk. The invention provides an anti-new coronavirus yolk neutralizing antibody IgY preparation which can be a spray, an eye drop, an oral capsule or an oral lozenge. The action mode is safe, large-scale production is easy, immunological rejection is not generated, and a safe, simple, sanitary, effective and economical personalized epidemic prevention mode is created to deal with epidemic situations.

Description

technical field [0001] The invention belongs to the field of epidemic prevention, and in particular relates to a SARS-CoV-2 egg yolk neutralizing antibody IgY spray for preventing and treating new coronary pneumonia. Background technique [0002] In 2019, the acute severe respiratory syndrome epidemic and highly infectious disease caused by the infection of the new coronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2, referred to as novel coronavirus, SARS-CoV-2, referred to as the new coronavirus) broke out. It has spread to the whole world. In China, more than 80,000 people have been infected and more than 5,000 people have died. In foreign countries, nearly 40 million people have been infected and more than 1 million people have died. Since there is no specific drug at present, it is very necessary to develop a specific drug against the new coronavirus. [0003] In SARS-CoV-2, the S protein on the surface of the virus mediates receptor recognition and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/12A61P31/14A61P11/00
CPCC07K16/10A61K9/12A61P31/14A61P11/00C07K2317/11C07K2317/76Y02A50/30
Inventor 赵凯
Owner 上海博满生物科技有限公司
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