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Anti-helicobacter pylori infection lactobacillus reuteri and application thereof

A Lactobacillus reuteri and anti-Helicobacter pylori technology, applied in the field of microorganisms, can solve problems such as difficulty in eradicating Helicobacter pylori, achieve good gastric acid resistance, improve eradication rate, inhibit proliferation and urease activity.

Pending Publication Date: 2022-05-13
南京益瑞兰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still difficult to achieve the eradication of Helicobacter pylori with only probiotics

Method used

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  • Anti-helicobacter pylori infection lactobacillus reuteri and application thereof
  • Anti-helicobacter pylori infection lactobacillus reuteri and application thereof
  • Anti-helicobacter pylori infection lactobacillus reuteri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this embodiment, the lactic acid bacteria screened from healthy humans are screened against Helicobacter pylori infection or inhibiting the growth of Helicobacter pylori. The steps and methods are as follows:

[0037] The assay method for the inhibition rate of Helicobacter pylori is: adopt agar diffusion method to measure the bacteriostatic activity of bacterial strains to Helicobacter pylori. The metronidazole solution with a concentration of 0.05 mg / mL was selected as the positive control, and the MRS liquid medium was used as the blank control.

[0038] The strain used in the experiment of inhibiting Helicobacter pylori is Helicobacter pylori SS1, and the culture medium of Helicobacter pylori SS1 adopts Columbia blood agar medium or liquid medium containing 5% defibrinated sheep blood (v / v). Helicobacter pylori SS1 culture conditions are: microaerophilic conditions (5% O 2 , 10%CO 2 , 85%N 2 ), cultured at 37°C for 72-96 hours. The Helicobacter pylori suspens...

Embodiment 2

[0046] In this example, the strains with high bacteriostatic rate screened in Example 1 were screened for acid resistance and bile salt resistance.

[0047] The 4 strains of lactic acid bacteria with a significantly high bacteriostatic rate in Example 1 were screened for acid resistance and bile salt resistance, and the test strains were respectively coated on the MRS agar medium containing 0.3% bovine bile salt and pH=3.5 for comparison According to the differences in the growth of colonies on the two agar media, lactic acid bacteria with high acid and bile salt resistance were screened out, and finally the strains were accurately identified by 16SrRNA sequencing. Bacterial ability, determine a strain numbered DRTR strain.

[0048] Table 2 Growth of strains on acidic and bile salt medium

[0049]

[0050] It can be seen from Table 2 that the activity of each strain decreased on acidic and bile salt medium, the main reason being that although this screening method is conve...

Embodiment 3

[0052] In this example, the DRTR strains screened in Example 2 with high ability to inhibit Helicobacter pylori, acid resistance and bile salt resistance were purified and preserved.

[0053] The DRTR strain obtained in Example 2 was streak cultured on an MRS agar culture machine for 48 hours, and a bright and plump single colony was selected and placed in 10 mL of liquid MRS medium, and cultured statically at 37°C for 12-16 hours to obtain purified bacteria liquid. Take 5mL of purified bacterial solution and add 30%-40% sterile glycerin at a volume of 1:1, mix well and distribute into 2ml sterile cryopreservation tubes, pre-cool at 4°C for 2 hours, and then pre-freeze at -20°C 4h, and finally transferred to -80°C refrigerator or liquid nitrogen for storage.

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Abstract

The invention discloses lactobacillus reuteri capable of resisting helicobacter pylori infection and application of the lactobacillus reuteri. The lactobacillus reuteri is identified as lactobacillus reuteri, the strain name is DRTR, the lactobacillus reuteri is preserved in the China Center for Type Culture Collection, the preservation date is October 19, 2020, and the preservation number is CCTCC NO: M2020597. The lactobacillus reuteri provided by the invention has good gastric acid resistance, can inhibit proliferation of helicobacter pylori and urease activity, and reduces adhesion of the helicobacter pylori to human gastric mucosa cells GES-1; the helicobacter pylori is prepared into a corresponding product for inhibiting helicobacter pylori infection, so that the growth of helicobacter pylori in a stomach environment can be inhibited; when the compound is combined with antibiotics to treat helicobacter pylori infection, the eradication rate can be remarkably increased, side effects in the treatment process are reduced, and the compound has very high application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Lactobacillus reuteri resistant to Helicobacter pylori infection and an application thereof. Background technique [0002] Helicobacter pylori (H. pylori i , Hp) species of helical rod-shaped microaerophilic bacteria that mainly colonize the gastric mucosa. It was first discovered in 1982 by two Australian scientists, Warren and Marshall, who won the 2005 Nobel Prize in Medical Physiology for their work. After years of research, it is well known that Helicobacter pylori is the main pathogenic factor of chronic gastritis, peptic ulcer, gastric cancer, gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and is related to some extraintestinal diseases. In 1994, the International Agency for Research on Cancer (IARC), which is part of the World Health Organization, designated Helicobacter pylori as a Class I carcinogen. However, studies have shown that e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A61K35/747A61K45/06A61P31/04A23L33/135C12R1/225
CPCC12N1/20C12N1/04A61K35/747A61K45/06A61P31/04A23L33/135A23V2002/00A23V2400/173A23V2200/3204
Inventor 贾长生卞徐磊施凌艳张少伦李珮铷
Owner 南京益瑞兰生物科技有限公司
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