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Scenedesmus oleaginus and culture application thereof

A technology of Scenedesmus and its cultivation method, which is applied in the field of biotechnology and bioenergy, and can solve problems such as unsatisfactory effects

Active Publication Date: 2022-05-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The oleaginous microalgae disclosed in the above two patents are directed against SO tolerant 2 Microalgae bred for specific properties such as low temperature resistance and low temperature resistance are not ideal when used in other aspects. Therefore, it is necessary to breed microalgae with more other functions, or to breed microalgae with multiple functions to make them more effective. suitable for industrial applications

Method used

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  • Scenedesmus oleaginus and culture application thereof
  • Scenedesmus oleaginus and culture application thereof
  • Scenedesmus oleaginus and culture application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Separation and domestication screening to obtain Scenedesmus TMJ-D3

[0027] (1) Obtaining the starting algae strain: In October 2016, 150 mL of water samples were retrieved from Tumen River, Tumen River City, Jilin Province, filtered with gauze to remove large impurities, and 50 mL of filtered water samples were inoculated into The enrichment culture was carried out in 200mL BG11 medium. The light intensity of the culture was 5000Lux, the temperature was 35°C, the light-dark cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of culture, the culture medium turned green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000 Lux, and the temperature is 35°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultiv...

Embodiment 2

[0032] Example 2 Identification of algae strains

[0033] The DNA of the obtained TMJ-D3 algae strain was extracted by CTAB method, and the 18S rDNA gene was cloned, and the obtained 3 positive clones were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Scenedesmus sp. It has the greatest similarity, its BLASTn value is 2562, and its Max index value is 99.3%. It can be determined that TMJ-D3 is Scenedesmus ( Scenedesmus sp. ).

Embodiment 3

[0034] Example 3 Application of Scenedesmus TMJ-D3

[0035] The algae liquid of TMJ-D3 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a photobioreactor. The OD of the culture medium after inoculation was 690 is 0.25. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 30v%, of which the NO content is 0.08v%. During the cultivation process, the light intensity was 8000Lux, the cultivation temperature was 35°C, the pH value was controlled at 7-8, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured to calculate the biomass production, and the total lipid content was measured by t...

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Abstract

The invention relates to an oil-producing scenedesmus strain and a culture application thereof, the oil-producing scenedesmus strain is oil-producing scenedesmus TMJ-D3, the classification name of the oil-producing scenedesmus strain is Scenedesmus sp., the oil-producing scenedesmus strain is preserved in China General Microbiological Culture Collection Center on February 5, 2018, and the preservation number of the oil-producing scenedesmus strain is CGMCC No.15299. The oil-producing scenedesmus strain is a strain of oil-producing scenedesmus TMJ-D3. The scenedesmus oleaginus provided by the invention is high in carbon sequestration efficiency and high in grease content, and particularly has the functions of resisting NOx and high temperature at the same time.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to oil-producing Scenedesmus and its cultivation application. Background technique [0002] Since the beginning of the 21st century, there is a close relationship between economic development and energy consumption. Energy security is a strategic issue for all countries. Finding a clean, economical, efficient and renewable new energy has become an urgent problem to be solved. Among them, bioenergy has always been an important reserve technology direction. With the intensification of the energy crisis, it has gradually become the focus of various countries and phycologists. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. The solar energy conversion ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64B01D53/56B01D53/62B01D53/84C12R1/89
CPCC12N1/12C12P7/64B01D53/84B01D53/56B01D53/62B01D2258/0283Y02A50/20
Inventor 师文静樊亚超张霖李晓姝廖莎王鹏翔朱化雷
Owner CHINA PETROLEUM & CHEM CORP
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