Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of DNA functionalized PBNPs nano-enzyme and application of DNA functionalized PBNPs nano-enzyme in preparation of targeted photo-thermal therapeutic agent

A nano-enzyme and functionalization technology, applied in the field of nano-biomedical materials, can solve the problems of lack of high specific targeting, achieve good clinical application prospects, solve incomplete photothermal treatment, and have good biological safety effects

Active Publication Date: 2022-05-27
CENT SOUTH UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the defects in the prior art, the first object of the present invention is to provide a DNA functionalized PBNPs nanozyme, which has photothermal therapy and targeting capabilities, and overcomes the limitations of existing PBNPs nanoparticles in photothermal therapy. There is no disadvantage of high specific targeting in the process, and at the same time, it has stronger enzyme-like catalytic activity than PBNPs, which can catalyze the generation of more active oxygen in cancer cells, so as to achieve the purpose of tumor ablation. The advantages and disadvantages of various treatment methods complement each other, so as to achieve better treatment effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of DNA functionalized PBNPs nano-enzyme and application of DNA functionalized PBNPs nano-enzyme in preparation of targeted photo-thermal therapeutic agent
  • Preparation method of DNA functionalized PBNPs nano-enzyme and application of DNA functionalized PBNPs nano-enzyme in preparation of targeted photo-thermal therapeutic agent
  • Preparation method of DNA functionalized PBNPs nano-enzyme and application of DNA functionalized PBNPs nano-enzyme in preparation of targeted photo-thermal therapeutic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Synthetic hydrophilic PBNPs precipitation:

[0031] Prepare 1mM Fe(NO 3 ) 3 and 1mM K 4 [Fe(CN) 6 ] 3 solution. When 1mM K 4 [Fe(CN) 6 ] 3 When heated to 60°C, 1mM Fe(NO 3 ) 3 The solution was added dropwise to the heated solution and the entire process was completed within 20 minutes. During this process, the color of the solution gradually changed to bright blue. Heating was continued for 5 minutes with stirring and then cooled to room temperature. Next, centrifugation was performed at 14,000 rpm for 15 minutes to obtain a composite. Subsequently, the precipitate was washed alternately with water and ethanol three times to remove inorganic salts. Subsequently, the washed PBNPs were freeze-dried.

[0032] The preparation method of aptamer C20AS1411 functionalized PBNPs nanozyme is as follows:

[0033] PBNPs (2.0 mg / mL) were combined with C20AS1411 (10 μM) in HEPES (50 mM, pH 7.4) in a saline solution (100 mM NaCl and 2 mM MgCl) in a total volume of 450 μ...

Embodiment 2

[0035] According to the method of Example 1, AS1411, A20AS1411 and T20AS1411 aptamers were used to replace C20AS1411 to obtain AS1411 functionalized PBNPs nanozyme, A20AS1411 functionalized PBNPs nanozyme and T20AS1411 functionalized PBNPs nanozyme, respectively.

Embodiment 3

[0037] The catalytic effect of aptamer C20AS1411 functionalized PBNPs nanozyme was verified as follows:

[0038] In 0.2M acetic acid-sodium acetate buffer (pH=5.0), 10 μg / mL PBNPs were mixed with 500 μM TMB and 1 mM H, respectively 2 O 2 The solutions were mixed and reacted at 25°C for 30 min. The UV absorption value of the above system at 652 nm was detected using a UV spectrophotometer.

[0039] The steps for evaluating the photothermal conversion of aptamer C20AS1411 functionalized PBNPs nanozyme are as follows:

[0040] Different concentrations of aptamer C20AS1411-functionalized PBNPs nanozymes at 0, 5, 10, 20, 40, 80 μg / mL were exposed to 808 nm laser (3 W / cm 2 ) for 600 seconds. To study the photostability, 80 μg / mL of the aptamer C20AS1411 functionalized PBNPs nanozyme with 808 nm laser at 3 W / cm 2 Irradiated for 600 s, then cooled for 600 s, and this cycle was repeated five times. In order to compare the effect of functionalization of aptamer C20AS1411 on the ph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a DNA functionalized PBNPs nano-enzyme, a preparation method thereof and application of the DNA functionalized PBNPs nano-enzyme in preparation of a targeted photo-thermal therapeutic agent. The DNA functionalized PBNPs nano-enzyme is formed by modifying a DNA aptamer on a Prussian blue nano-particle through coordination complexation, has photo-thermal therapy and targeting capabilities, overcomes the defect that the existing PBNPs nano-particle does not have high-specificity targeting in the photo-thermal therapy process, also has stronger enzyme-like catalytic activity compared with PBNPs, and can be used for preparing the photothermal therapy nano-enzyme for the photothermal therapy of the prussian blue nano-particle, so that the photothermal therapy of the prussian blue nano-particle can be realized by using the DNA functionalized PBNPs nano-enzyme for the photothermal therapy of the prussian blue nano-particle. The nano-enzyme can catalyze generation of more active oxygen in cancer cells so as to achieve the purpose of tumor ablation, and the nano-enzyme realizes advantage and disadvantage complementation of a plurality of treatment modes and can achieve a better treatment effect.

Description

technical field [0001] The invention relates to a targeted photothermal therapeutic agent, in particular to a DNA-functionalized PBNPs nano-targeted photothermal therapeutic agent, a preparation method and application thereof in the preparation of a targeted photothermal therapeutic agent, and belongs to nano-biomedicine material technology field. Background technique [0002] Cancer is currently the disease with the highest mortality rate in the world, while lung cancer is one of the malignant tumors with the fastest growing incidence and mortality, and the greatest threat to human health and life. Traditional cancer treatment methods, including radiotherapy, chemotherapy and surgery, all have obvious toxic side effects or limitations. Over the past few years, photothermal therapy has emerged as a prospective adjuvant therapy for cancer. When near-infrared laser excitation is combined with photothermal materials in cancerous areas, it can kill ordinary tumor cells at high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/54A61K33/26A61K41/00A61P35/00B82Y5/00B82Y30/00B82Y40/00
CPCA61K47/549A61K41/0052A61K33/26A61P35/00B82Y5/00B82Y30/00B82Y40/00Y02A50/30
Inventor 邓春艳陈苗
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com