Tussah silk fibroin composite nanofiber for wound repair

A technology of tussah silk fibroin and composite nanofibers, which is applied in the direction of cellulose/protein conjugated artificial filaments, fiber treatment, fiber chemical characteristics, etc., can solve the problems of difficult and unavoidable nanofibers, and achieve the improvement of mechanical strength, The effect of suppressing scars and expanding the range of application

Active Publication Date: 2022-05-27
HENAN INST OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tussah silk is abundant in China. As a natural polymer protein, tussah silk protein contains an Arg-Gly-Asp (RGD) sequence, and the RGD sequence has excellent cell adhesion, which can promote cell proliferation and play a role in wound repair. The field of medical materials has a wide range of application values; carboxymethyl chitosan is an important water-soluble chitosan derivative, which has the functions of promoting wound healing, hemostasis, inhibiting scars, analgesia and antibacterial, and it has good Biocompatibility and biodegradability are widely used in the field of biomedical materials; gelatin, as a polypeptide mixture, has good biocompatibility, degradability and water solubility; it can be widely used in medical hemostatic ma

Method used

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  • Tussah silk fibroin composite nanofiber for wound repair
  • Tussah silk fibroin composite nanofiber for wound repair
  • Tussah silk fibroin composite nanofiber for wound repair

Examples

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Effect test

Embodiment 1

[0024] A kind of tussah silk fibroin composite nanofiber for wound repair, the preparation method specifically comprises the following steps:

[0025] (1) Preparation of regenerated tussah silk fibroin solution: put tussah silk in 0.5% Na 2 CO 3 After repeating degumming 3 times in the solution, drying and shredding, utilizing lithium thiocyanate solution to dissolve tussah silk fibroin, and then after dialysis concentration, adding absolute ethanol to the concentrated solution to obtain regenerated tussah silk fibroin solution A;

[0026] (2) adding carboxymethyl chitosan to the regenerated tussah silk fibroin solution A, stirring and dissolving at room temperature to obtain solution B;

[0027] (3) Add gelatin to solution B and stir to dissolve to obtain solution C;

[0028] (4) Add nano-silver into solution C, carry out ultrasonic dispersion for 30-60min, and obtain solution D;

[0029] (5) Spinning the solution D with an electrospinning device to obtain functional tussa...

Embodiment 2

[0034] A kind of tussah silk fibroin composite nanofiber for wound repair, the preparation method specifically comprises the following steps:

[0035] (1) Preparation of regenerated tussah silk fibroin solution: put tussah silk in 0.5% Na 2 CO 3 After repeating degumming 3 times in the solution, drying and shredding, utilizing lithium thiocyanate solution to dissolve tussah silk fibroin, and then after dialysis concentration, adding absolute ethanol to the concentrated solution to obtain regenerated tussah silk fibroin solution A;

[0036] (2) adding carboxymethyl chitosan to the regenerated tussah silk fibroin solution A, stirring and dissolving at room temperature to obtain solution B;

[0037] (3) Add gelatin to solution B and stir to dissolve to obtain solution C;

[0038] (4) Add nano-silver into solution C, carry out ultrasonic dispersion for 30-60min, and obtain solution D;

[0039] (5) Spinning the solution D with an electrospinning device to obtain functional tussa...

Embodiment 3

[0044] A kind of tussah silk fibroin composite nanofiber for wound repair, the preparation method specifically comprises the following steps:

[0045] (1) Preparation of regenerated tussah silk fibroin solution: put tussah silk in 0.5% Na 2 CO 3 After repeating degumming 3 times in the solution, drying and shredding, utilizing lithium thiocyanate solution to dissolve tussah silk fibroin, and then after dialysis concentration, adding absolute ethanol to the concentrated solution to obtain regenerated tussah silk fibroin solution A;

[0046] (2) adding carboxymethyl chitosan to the regenerated tussah silk fibroin solution A, stirring and dissolving at room temperature to obtain solution B;

[0047] (3) Add gelatin to solution B and stir to dissolve to obtain solution C;

[0048] (4) Add nano-silver into solution C, carry out ultrasonic dispersion for 30-60min, and obtain solution D;

[0049] (5) Spinning the solution D with an electrospinning device to obtain functional tussa...

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Abstract

The invention discloses a tussah silk fibroin composite nanofiber for wound repair. The composite nanofiber is prepared from tussah silk fibroin, carboxymethyl chitosan, gelatin and an antibacterial agent according to a proper ratio through an electrostatic spinning technology under a certain spinning condition. Meanwhile, ethanol is also added into the tussah silk fibroin solution, so that the tussah silk fibroin in the composite nanofiber membrane is ensured to form a beta-folded structure from an angelica-free curled structure; the problems that in the prior art, a nanofiber membrane formed by spinning is immersed in an ethanol solution, so that the nanofiber membrane is adhered, and nanofibers are dissolved in ethanol are solved, meanwhile, the mechanical strength of the nanofibers is improved, and the application range of the nanofiber membrane is widened; the obtained nanofiber has a good application prospect in the aspect of wound repair.

Description

technical field [0001] The invention belongs to the technical field of composite nanofibers, in particular to an tussah silk fibroin composite nanofiber used for wound repair. Background technique [0002] The production of tussah silk in my country is abundant. As a natural polymer protein, tussah silk protein contains Arg-Gly-Asp (RGD) sequence, and the RGD sequence has excellent cell adhesion, which can promote cell proliferation and improve wound repair. It has a wide range of application value in the field of medical materials; carboxymethyl chitosan is an important water-soluble chitosan derivative, which has the functions of promoting wound healing, hemostasis, scar inhibition, analgesia and bacteriostasis. Biocompatibility and biodegradability are widely used in the field of biomedical materials; gelatin, as a peptide mixture, has good biocompatibility, degradability and water solubility; it can be widely used in medical hemostatic materials, but simple The aqueous gel...

Claims

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Application Information

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IPC IPC(8): D01F8/02D01F8/18D01F1/10A61L15/18A61L15/28A61L15/32A61L15/44A61L15/46D01D5/00
CPCD01F8/02D01F8/18D01F1/103A61L15/32A61L15/28A61L15/18A61L15/46A61L15/44D01D5/003A61L2400/12A61L2300/104A61L2300/102A61L2300/232A61L2300/404A61L2300/412A61L2300/418C08L89/00C08L5/08Y02A50/30
Inventor 张显华冯向伟于宾刘杰邹清云陈莉娜周金龙
Owner HENAN INST OF ENG
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