Tussah silk fibroin composite nanofiber for wound repair

Abstract

The invention discloses a tussah silk fibroin composite nanofiber for wound repair. The composite nanofiber is prepared from tussah silk fibroin, carboxymethyl chitosan, gelatin and an antibacterial agent according to a proper ratio through an electrostatic spinning technology under a certain spinning condition. Meanwhile, ethanol is also added into the tussah silk fibroin solution, so that the tussah silk fibroin in the composite nanofiber membrane is ensured to form a beta-folded structure from an angelica-free curled structure; the problems that in the prior art, a nanofiber membrane formed by spinning is immersed in an ethanol solution, so that the nanofiber membrane is adhered, and nanofibers are dissolved in ethanol are solved, meanwhile, the mechanical strength of the nanofibers is improved, and the application range of the nanofiber membrane is widened; the obtained nanofiber has a good application prospect in the aspect of wound repair.

Description

technical field [0001] The invention belongs to the technical field of composite nanofibers, in particular to an tussah silk fibroin composite nanofiber used for wound repair. Background technique [0002] The production of tussah silk in my country is abundant. As a natural polymer protein, tussah silk protein contains Arg-Gly-Asp (RGD) sequence, and the RGD sequence has excellent cell adhesion, which can promote cell proliferation and improve wound repair. It has a wide range of application value in the field of medical materials; carboxymethyl chitosan is an important water-soluble chitosan derivative, which has the functions of promoting wound healing, hemostasis, scar inhibition, analgesia and bacteriostasis. Biocompatibility and biodegradability are widely used in the field of biomedical materials; gelatin, as a peptide mixture, has good biocompatibility, degradability and water solubility; it can be widely used in medical hemostatic materials, but simple The aqueous gel...

AI Technical Summary

Problems solved by technology

[0002] Tussah silk is abundant in China. As a natural polymer protein, tussah silk protein contains an Arg-Gly-Asp (RGD) sequence, and the RGD sequence has excellent cell adhesion, which can promote cell proliferation and play a role in wound repair. The field of medical materials has a wide range of application values; carboxymethyl chitosan is an important water-soluble chitosan derivative, which has the functions of promoting wound healing, hemostasis, inhibiting scars, analgesia and antibacterial, and it has good Biocompatibility and biodegradability are widely used in the field of biomedical materials; gelatin, as a polypeptide mixture, has good biocompatibility, degradability and water solubility; it can be widely used in medical hemostatic materials, but pure The gelatin aqueous solution is in a colloidal state at room temperature, and it is difficult to prepare nanofibers by electrospinning technology; at the same time, using tussah silk fibroin, carboxymethyl chitosan, and gelatin as raw materials are prepared by electrospinning technology for wound repair The nanofibers of tussah silk fibroin have not been reported yet, and when preparing nanofibers with tussah silk fibroin as raw material in the prior art, in order to make the tussah silk fibroin nanofibers form a β-sheet structure, the obtained nanofibers are often soaked in ethanol solution , but this method will inevitably cause the dissolution and adhesion of nanofibers, which will affect the properties of the obtained nanofibers.

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