Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Engineering bacterium for converting branched chain amino acid and application of engineering bacterium in preparation of product for treating maple sugar urine

A technology of branched-chain amino acids and engineering bacteria, applied to bacteria used in food preparation, genetic engineering, medical preparations containing active ingredients, etc., can solve the problem of lack of MSUD engineering probiotics, affecting the growth and mental health of MSUD patients, Improve the quality of life, important clinical treatment value and social benefits

Active Publication Date: 2022-05-31
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term low-protein diet treatment will affect the growth and mental health of MSUD patients, and the quality of life of patients is poor
However, there is no research report on engineering probiotics that can treat MSUD

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Engineering bacterium for converting branched chain amino acid and application of engineering bacterium in preparation of product for treating maple sugar urine
  • Engineering bacterium for converting branched chain amino acid and application of engineering bacterium in preparation of product for treating maple sugar urine
  • Engineering bacterium for converting branched chain amino acid and application of engineering bacterium in preparation of product for treating maple sugar urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Construction of engineering probiotics MS312

[0040] The starting strain of Lactobacillus acidophilus NCFM is used to integrate the leucine dehydrogenase gene LeuDH and the α-hydroxyacid dehydrogenase gene MorA on the genome, including the following steps:

[0041] In this example, the whole gene synthesis and sequencing were completed by Shanghai Sangon Bioengineering Co., Ltd. Molecular biology experiments, including preparation of competent cells, electrotransformation, etc., were carried out according to the "Molecular Cloning Experiment Guide" (third edition).

[0042] Design the gene fragment MSUD1 containing promoter P32 (SEQ ID NO.3), leucine dehydrogenase gene LeuDH (SEQ ID NO.1) and α-hydroxyacid dehydrogenase gene MorA (SEQ ID NO.2), The DNA fragment was obtained by total gene synthesis and constructed into the cloning vector pUC57 to obtain the pUC57-MSUD1 vector.

[0043] Lactobacillus acidophilus NCFM genome was used as a template, L-For and L-Rev we...

Embodiment 2

[0051] Example 2 Effect of engineering probiotic MS312 on maple syrup urine disease mice

[0052] The mouse model of maple syrup urine disease was purchased from a biotechnology company, and the genotype was C57BL / 6J-Bckdha em1 . Under normal feeding conditions, the model mice had typical symptoms of maple syrup urine disease. The experiment used 6-week-old C57BL / 6J-Bckdha em1 The mice were fed with a non-branched-chain amino acid feed, and 100 μL of purified water containing leucine, isoleucine and valine was regularly orally administered every day as a branched-chain amino acid supplement. After the start of the animal experiment, the mice were divided into 3 groups, and they were fed with 200 μL of normal saline, NCFM probiotics or MS312 engineering probiotics twice a day at regular intervals, and the dosage of probiotics was about 1*10 10 cfu / mouse. Blood and urine samples were collected after probiotic feeding for 14 days, and the branched-chain amino acid concentrati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an engineering bacterium for converting branched chain amino acid and application of the engineering bacterium in preparation of a product for treating maple sugar urine. According to the invention, a carrier containing a gene LeuDH and a gene MorA is constructed, and the carrier is transferred into lactobacillus in a homologous recombination mode. Through verification, the recombinant strain can effectively express leucine dehydrogenase and alpha-hydroxy acid dehydrogenase and realize the degradation effect on branched chain amino acid, is expected to be used as an engineering bacterium to be applied to preparation of drugs for treating maple sugar urine diseases, and has important significance on clinical treatment of maple sugar urine diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to an engineering bacterium for converting branched-chain amino acid into α-hydroxy acid, a bacterial agent of the engineering bacterium, a pharmaceutical composition and its application as a branched-chain amino acid degradation agent, in particular to its Application in preparation of products for treating maple syrup urine disease. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Maple syrup urine disease (MSUD), also known as branched-chain ketoaciduria, was included in the "First List of Rare Diseases" issued in 2018. Maple syrup urine disease is an autosomal recessive gen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/21C12N15/74C12N15/90C12N15/53A23L33/135A23L2/52A23G3/36A61K35/747A61P43/00C12R1/23
CPCC12N9/0016C12N9/0006C12N15/746C12N15/902C12Y104/01009C12Y101/0103A23L33/135A23L2/52A23G3/366A61K35/747A61P43/00C12N2800/22A23V2002/00A23V2400/113A23V2200/30Y02A50/30
Inventor 曹广祥付加芳马欣王杰
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com