Fluorescence detection method for copper ions

A fluorescence detection and copper ion technology, applied in the field of analysis and detection, can solve the problems that the system to be tested is easily polluted, the solid particles are not easy to be recovered, and the cost of the fluorescent probe is high, and achieves favorable fluorescence detection performance, good fluorescence detection performance, and method. Simple and easy effects

Pending Publication Date: 2022-05-31
HEBEI UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When performing fluorescence detection in the liquid phase, due to the influence of solvation, the detection performance is limited, and there are also disadvantages such as a large amount of waste liquid and easy contamination of the system to be tested.
Adding solid particles into the liquid phase can increase the fluorescence intensity, but there are disadvantages such a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence detection method for copper ions
  • Fluorescence detection method for copper ions
  • Fluorescence detection method for copper ions

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

Embodiment 1

[0040] (1) The zirconium sheet (15mm×30mm×0.2mm) was ultrasonically cleaned with ethanol and deionized water. Weigh 0.1 g of ammonium fluoride

A solution of amino coupling agent with a concentration of 0.2M was prepared. Place the sample ZNAF level in a weighing bottle and take 100 μl of amino coupling agent to dissolve

(3) the detection of copper ion

1. with 0.1M acetic acid-sodium acetate buffer solution (pH=5.4) preparation concentration is 0.001, 0.01, 0.1, 1, 10,

A solution of amino coupling agent with a concentration of 0.2M was prepared. Place the sample ZNAF level in a weighing bottle and take 100 μl of amino coupling agent to dissolve

3. under the condition that excitation wavelength is 550nm (FL-7000 fluorescence spectrophotometer, Japan Hitachi company) test with

4. with copper sulfate and tap water preparation copper ion concentration is that the solution of 1.0 μM is made solution to be tested, this solution is combined with

1. with 0.1M...

Example Embodiment

2. by sample ZNAF-NH

[0050] (1) ZNAF was prepared with the process conditions and steps of Example 1 (1), and calcined at 400° C. for 2 hours.

(2) with γ-aminopropyl trimethoxysilane and 95% (volume percent) ethanol preparation concentration is the amino group of 0.5M

(3) prepare detection sample with the rhodamine B solution of 15mg / L, with embodiment 1 (3) process step, can carry out copper ionization

Example Embodiment

Embodiment 3

[0054] (1) ZNAF was prepared with the process conditions and steps of Example 1 (1), and calcined at 600° C. for 1 hour.

(2) with γ-aminopropyl triethoxysilane and 85% (volume percent) ethanol preparation concentration is the amino group of 0.01M

(3) prepare detection sample with the rhodamine B solution of 5mg / L, with embodiment 1 (3) process step, can carry out copper ion

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a fluorescence detection method for copper ions. According to the method, an amino-functionalized zirconium dioxide nanotube array film is used as a carrier, rhodamine B is used as a fluorescent probe, copper ions and rhodamine B are immobilized on the amino-functionalized zirconium dioxide nanotube array film carrier to prepare a detection sample, and the copper ions can be detected by testing the fluorescence intensity of the detection sample. The method is simple and easy to implement, good in detection performance, low in cost and small in waste liquid amount, and the amino-functionalized zirconium dioxide nanotube array film carrier can be regenerated and reused.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to a fluorescence detection method for copper ions. Background technique [0002] Because heavy metal elements are widely used in many fields, a large number of heavy metal ions enter the water body, causing heavy metal ion pollution, which will cause serious harm to human health, animals and plants. For example, copper is an important component of human health and many biological tissues. This micronutrient element plays a vital role in bone formation, cellular respiration, and synthesis of hemoglobin and elastin; however, if copper ions cannot maintain At appropriate physiological concentrations, it can cause serious syndromes such as osteoporosis, Wilson's disease and Alzheimer's disease. [0003] The detection of heavy metal ions is the basis for preventing and controlling heavy metal ion pollution. Therefore, it is of great significance to detect copper ions. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 赵建玲刘东鑫王西新李奉霖刘冬
Owner HEBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products