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Application of TPPI gene in regulation and control of plant root development and promotion of plant jasmonic acid accumulation

A technology of plant roots and jasmonic acid, applied in the field of molecular biology, can solve problems such as no reports, and achieve the effects of promoting accumulation, reducing labor consumption, and reducing fertilizer pollution

Active Publication Date: 2022-06-07
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the role of TPPI genes in promoting plant root development or promoting plant jasmonic acid accumulation.

Method used

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  • Application of TPPI gene in regulation and control of plant root development and promotion of plant jasmonic acid accumulation
  • Application of TPPI gene in regulation and control of plant root development and promotion of plant jasmonic acid accumulation
  • Application of TPPI gene in regulation and control of plant root development and promotion of plant jasmonic acid accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the expression level detection of TPPI gene

[0034] (1) Primer design

[0035] Design primers for real-time fluorescence quantification according to the CDS sequence of the TPPI gene, and the primer sequences are:

[0036] Forward primer: 5'-GAATCAACGCTTGGGTAGATTC-3' (SEQ ID No. 2)

[0037] Reverse primer: 5'-TTGACATGAAAGCTTTGTCTGG-3' (SEQ ID No. 3)

[0038] (2) extracting the total RNA of wild-type Arabidopsis thaliana, namely Arabidopsis thaliana (Arabidopsis thaliana, Columbia ecotype) (hereinafter referred to as WT) and transgenic plants OE4 and OE5, and obtaining its cDNA by reverse transcription, Take it as a template, and use the oligonucleotide sequences of SEQ ID No.2 and SEQ ID No.3 as primers to carry out real-time fluorescence quantitative PCR amplification to obtain PCR amplification products. The nucleotide sequence is as shown in SEQ ID No.4. Show. The detection results of TPPI gene expression level are as follows: figure 1 shown. The...

Embodiment 2

[0039] Example 2. The effect of TPPI gene on the growth of plant taproot in a plant artificial climate chamber

[0040] The seeds of transgenic plants OE4, OE5 and wild-type WT were sterilized with 6.5% NaClO for 15 min respectively, the NaClO was discarded, and then the seeds were washed 3-5 times with sterilized sterile water, and then evenly dotted on MS solid medium for vertical cultivation, The solid medium used is MS519 solid medium of Phytotech company (sucrose content is 5g / L, agar dosage is 10g / L) and MS531 solid medium (5g / L sucrose, 10g / L agar), MS531+KNO 3 Add 10 mM KNO to MS531 solid medium 3 . After being placed in a 4°C refrigerator for 3 days, it was placed in an artificial climate chamber for vertical growth. The culture temperature in the artificial climate chamber was 22°C, the light intensity was 6000 lux, the cycle was 16h light / 8h dark, and the relative humidity was 50%. After 10 days of growth, the phenotype was observed and photographed, and the resul...

Embodiment 3

[0043] Example 3. The effect of TPPI gene on the growth of plant taproot in a plant incubator

[0044] The seeds of tppi1 mutants, transgenic plants OE4, OE5 and wild-type WT were sterilized with 6.5% NaClO for 15 min respectively, the NaClO was discarded, and then the seeds were washed 3-5 times with sterilized sterile water, and then evenly dotted on MS solids for culture. Substrate vertical culture, the solid medium used is Phytotech's MS519 solid medium and MS531 solid medium, MS531+KNO 3 Add 10 mM KNO to MS531 solid medium 3 . After being placed in a refrigerator at 4°C for 3 days, it was placed in a light incubator for vertical growth. The culture temperature of the light incubator was 22°C, the light intensity was 9000 lux, the photoperiod was 16h light / 8h dark, and the relative humidity was 50%. After 14 days of growth, the phenotype was observed and photographed, and the results were as follows Figure 4 shown.

[0045] right Figure 4 The root length of the plan...

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Abstract

The invention discloses application of a TPPI gene to regulation and control of plant root development and promotion of plant jasmonic acid accumulation, and belongs to the technical field of molecular biology. The invention provides an application of a TPPI gene in regulation and control of plant root development and an application of the TPPI gene in promotion of plant jasmonic acid accumulation under normal conditions and mild cold stress conditions. A nucleotide sequence of the TPPI gene is shown as SEQ ID NO. 1. The TPPI gene can be directly over-expressed to promote the growth of the main root of the plant, so that the labor consumption caused by deep application of soil can be reduced, less fertilizer can be applied, the fertilizer pollution can be reduced, and the TPPI gene has potential application in nitrate nitrogen-loving crops and soil in which nitrogen is easy to lose; according to the invention, accumulation of jasmonic acid in plants under normal conditions and mild cold stress conditions can be promoted directly through overexpression of the TPPI gene, and the plants can be helped to resist cold and adapt to mild cold stress.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to the application of TPPI gene in regulating plant root development. Background technique [0002] The root system of plants is the main organ for plants to absorb nutrients and water. Therefore, the root system is extremely important for the growth and development of plants. Soil nutrient status affects root growth, and increasing nutrient supply can not only promote root growth, but also change root morphology. Nutrient concentration in the soil is high, and the root system is concentrated. The effect of nitrogen on roots is particularly obvious. As an important nutrient element for plants, nitrogen is mainly composed of inorganic nitrogen nitrate (NO). 3 - ) and ammonia nitrogen (NH 4 + )exist. The nitrogen absorbed by the root system can not only be transported to the shoots through the xylem conduits to participate in various physiological metabolisms, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/16C12N15/82A01H5/06A01H5/00A01H6/20
CPCC12N9/16C12Y301/03012C12N15/8273C12N15/8243Y02A40/146
Inventor 林清芳齐世连巩佳昕
Owner XUZHOU NORMAL UNIVERSITY
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