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Kelp polypeptide, antibacterial repairing whitening toothpaste, preparation method and application

A technology of kelp and toothpaste, which is applied in the field of preparation of antibacterial, repairing and whitening toothpaste, kelp polypeptide, and achieves the effect of broad application prospects

Inactive Publication Date: 2022-06-28
YIWU AISHANG COMMODITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the enzymatic hydrolysis and extraction of active substances in kelp have mostly focused on laminarin, mannitol, dietary fiber, and iodine, while there are fewer reports on laminarin and active peptides

Method used

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  • Kelp polypeptide, antibacterial repairing whitening toothpaste, preparation method and application
  • Kelp polypeptide, antibacterial repairing whitening toothpaste, preparation method and application
  • Kelp polypeptide, antibacterial repairing whitening toothpaste, preparation method and application

Examples

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preparation example Construction

[0028] Preparation of kelp polypeptide

[0029] 1. Materials and methods

[0030] 1. Preprocessing

[0031] The dried commercially available Xiapu kelp was pulverized with a pulverizer and passed through a 40-mesh sieve to obtain kelp powder, which was sealed and stored at 4°C in the dark.

[0032] 2. Extract protein

[0033] Use low temperature freeze-thaw combined with ultrasonic to break cells of kelp powder, and then extract the broken cell liquid to obtain the protein liquid of kelp.

[0034] In a specific extraction process of kelp powder protein, accurately weigh 100g of kelp powder, add 300mL of distilled water, fully stir and disperse, let it stand for 1 to 5 days, and quickly freeze at -20°C for 4 hours, then transfer to 4°C environment. After being thawed in the middle, put it in an ultrasonic cleaner with 120W ultrasonic power for 30 minutes; then transfer to -20°C for rapid freezing for 4h, transfer to 4°C for thawing, and after 120W ultrasonic treatment for ...

Embodiment 7

[0101] The preparation method of the polypeptide components in Example 7 is as follows: respectively accurately weigh 0.5 mg of kelp polypeptides T3, T6, T8, and T9 and dissolve them in 100 μL of pH6.8 trimaleic acid buffer to serve as the supply of 10 kinds of kelp polypeptides. The test solution was mixed with 1mL of 10ppm iron ion solution and 1mL of 0.1% (wt / vol) ascorbic acid solution in 2M hydrochloric acid by vortexing, incubated at room temperature for 5min, and 0.9mL of 11.3% trichloroacetic acid ( TCA), centrifuge at 2500g for 10min; take the precipitate and mix, wash with deionized water until neutral.

Embodiment 8

[0102] The preparation method of the polypeptide components in Example 8 is as follows: respectively accurately weigh 2 mg of kelp polypeptides T3, T6, T8, and T9 in 1 mL of distilled water, respectively, and mix them with 1 mL of 0.2 mM copper sulfate solution in 20 mL of pH6.8 Sanma. Add 1 mL of 11.3% TCA to the mixed solution, centrifuge at 2500 g for 10 min, take the precipitate and mix, and wash with deionized water until neutral.

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Abstract

The invention discloses kelp polypeptide, antibacterial repairing and whitening toothpaste, a preparation method and application. The kelp polypeptide composition comprises at least one of kelp polypeptides as shown in SEQ ID NO.1-10, and a peptide variant, a peptide conjugate / chelate molecule, a derivative and a salt formed by peptide of the kelp polypeptide. Wherein part of polypeptide has antioxidant activity and iron-copper ion chelating performance, has bacteriostatic action on common tooth pathopoiesis and is insensitive to common tooth probiotics, in-vitro dental plaque experiments and whitening experiments verify that the polypeptide has bacteriostatic, cleaning, whitening and repairing functions on teeth, and the polypeptide has very wide application prospects.

Description

technical field [0001] The present application relates to the technical field of kelp polypeptides, in particular to kelp polypeptides, antibacterial repair and whitening toothpaste, preparation methods and applications. Background technique [0002] The kelp belongs to the genus Kelpaceae, the family Kelpidae, and is delicious in taste and high in nutritional value. In recent years, the enzymatic extraction of active substances from kelp has mostly focused on laminarin, mannitol, dietary fiber and iodine, etc., while there are few reports on laminarin and active peptides. The prior art found that kelp protein has inhibitory effect on Escherichia coli, Aerobacter aerogenes, Staphylococcus aureus, and has a certain antihypertensive effect on hypertensive model rats; and, kelp extract has an inhibitory effect on Corynebacterium michiganum ring rot. Subspecies have bacteriostatic effect. SUMMARY OF THE INVENTION [0003] The inventors of the present application have conduct...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/36C07K1/34C07K1/22C07K1/16C07K1/14A61K8/64A61P1/02A61P31/02A61P31/04A61Q11/00
CPCC07K14/405C07K1/36C07K1/34C07K1/22C07K1/16C07K1/14A61K8/645A61Q11/00A61P31/02A61P31/04A61P1/02
Inventor 赵国盛
Owner YIWU AISHANG COMMODITY
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