Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of molecularly imprinted SPR (Surface Plasmon Resonance) sensor for detecting amphetamine drugs

A technology of molecular imprinting and amphetamines, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as the inability to form a uniform and effective sensitive film on the surface of the SPR sensor, limiting the detection stability, reproducibility, sensitivity, and poor dispersion of the sensor , to achieve good application potential, improve detection sensitivity and selectivity, and controllable thickness

Pending Publication Date: 2022-06-28
GUANGZHOU UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, MIPs films with controllable thickness can be prepared on the surface of the SPR chip by selecting an appropriate polymerization method. However, these methods mostly use precipitation polymerization to synthesize MIPs with cumbersome operations and poor dispersion, which cannot form a uniform and effective sensitive film on the surface of the SPR sensor. , which greatly limits the detection stability, reproducibility and sensitivity of the sensor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of molecularly imprinted SPR (Surface Plasmon Resonance) sensor for detecting amphetamine drugs
  • Preparation method of molecularly imprinted SPR (Surface Plasmon Resonance) sensor for detecting amphetamine drugs
  • Preparation method of molecularly imprinted SPR (Surface Plasmon Resonance) sensor for detecting amphetamine drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0055] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0056] see Figure 1-8 , the preparation method of the molecularly imprinted SPR sensor for detecting amphetamine drugs that the present invention proposes, comprises the following steps:

[0057] S1. Preparation of experimental drugs

[0058] The drugs required for the experiment include cover glass (18×18mm), (±)3,4-methylenedioxyamphetamine (MDA), (±)3,4-methylenedioxymethamphetamine (MDMA), (±)-Methamphetamine (MA), Ketamine hyd...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of amphetamine drug detection, and particularly discloses a preparation method of a molecularly imprinted SPR (Surface Plasmon Resonance) sensor for detecting amphetamine drugs, which comprises the following steps: depositing a layer of 5nm chromium and 50nm gold film on cover glass to prepare an SPR chip; the preparation method comprises the following steps: firstly, preparing a template, then sufficiently washing and drying, mounting the template on a prism of an electrochemical-SPR (Surface Plasmon Resonance) combined system by utilizing the adhesion effect of cedar oil, and taking 3, 4-methylenedioxy phenylethylamine (MDEA) as a template molecule and dopamine (DA) as a functional monomer. According to the preparation method of the molecularly imprinted SPR sensor for detecting amphetamine drugs, the thickness and uniformity of a polymer film are cooperatively adjusted through electrochemistry and SPR signals, so that experimental steps are effectively reduced; meanwhile, dopamine has more amino groups and hydroxyl groups, recognition sites are rich, and the detection sensitivity and selectivity of the sensor are effectively improved; the method has a good detection effect on amphetamine drugs MDA and MDMA, and can tolerate interference of various illegal drugs and other substances.

Description

technical field [0001] The invention relates to the technical field of amphetamine drugs, in particular to a preparation method of a molecularly imprinted SPR sensor for detecting amphetamine drugs. Background technique [0002] Amphetamine-type stimulants (ATS), derived from beta-phenethylamine, are a widely abused class of illicit synthetic drugs, the most well-known of which are amphetamine (A), methamphetamine (MA or "ice"),3,4 - Methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA or "ecstasy") and other psychostimulants. [0003] Currently, techniques that can be used for the quantitative determination of ATS include thin-layer chromatography, chromatography-mass spectrometry (GS-MS, HPLC-MS), Raman spectroscopy, Fourier transform infrared (FTIR) spectroscopy, capillary electrophoresis (CE), Techniques such as nuclear magnetic resonance (NMR) and immunoassays. [0004] Surface plasmon resonance (SPR) sensors monitor the intermolecular interactions...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 韩冬雪李陈牛利
Owner GUANGZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com