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Method for improving conversion rate of grease yeast and regulating composition of grease fatty acid by pretreating alcohol fermentation wastewater

A technology for oily yeast and fermented wastewater, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as high sulfate and ammonia nitrogen content, achieve high added value, simple and feasible process, and improve The effect of conversion efficiency

Pending Publication Date: 2022-07-01
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pretreatment methods such as dilute acid treatment and ammonia fiber blasting will also make the content of sulfate and ammonia nitrogen in the wastewater very high

Method used

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  • Method for improving conversion rate of grease yeast and regulating composition of grease fatty acid by pretreating alcohol fermentation wastewater
  • Method for improving conversion rate of grease yeast and regulating composition of grease fatty acid by pretreating alcohol fermentation wastewater
  • Method for improving conversion rate of grease yeast and regulating composition of grease fatty acid by pretreating alcohol fermentation wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Activated carbon was used to detoxify the ethanol fermentation wastewater, and Trichosporoncutaneum was used for fermentation after the treatment.

[0016] Insert Trichosporon cutaneum from the test tube into 50 mL of activation medium, and at 20° C., activate and cultivate for 24 hours to obtain Trichosporon cutaneum seed liquid. The activated medium is: glucose 20g / L, peptone 10g / L, yeast powder 10g / L L, the balance is water.

[0017] In the control group, activated carbon was not used in the medium of ethanol fermentation wastewater for detoxification, and the seed liquid was directly connected to the medium for fermentation.

[0018] Activated carbon was added to the ethanol fermentation wastewater in the proportions of 0.5wt% and 5wt% respectively, and the paddle stirring method was adopted. The stirring condition was 50rpm / min for 0.5h. After the stirring was completed, the activated carbon was removed by filtration, and the filtrate was taken to determine the ald...

Embodiment 2

[0027] The ethanol fermentation wastewater was detoxified by attapulgite, and then fermented by Rhodosporidium toruloides after the treatment.

[0028] Put Rhodosporidium toruloides from the test tube into 50 mL of activation medium, 20 ° C, activate and cultivate for 24 hours, to obtain Rhodosporidium toruloides seed liquid, the activation medium is: glucose 20g / L, peptone 10g / L, yeast powder 10g / L L, the balance is water.

[0029] In the control group, the attapulgite was not used for detoxification in the ethanol fermentation wastewater medium, and the seed liquor was directly fermented.

[0030] Attapulgite was added to the ethanol fermentation wastewater in a proportion of 2wt%, and the attapulgite was respectively stirred by anchor stirring for 0.5 and 2 hours, and the rotation speed was 200 rpm / min. (furfural, 5-hydroxymethylfurfural), phenols, ammonia nitrogen content, and measure the light transmittance; after adjusting the pH, the oil yeast seed liquid was connected...

Embodiment 3

[0040] The butanol fermentation wastewater was detoxified by polar macroporous adsorption resin, and Rhodotorula glutinis and Lipomyces starkeyi were used for mixed bacterial fermentation after the treatment.

[0041] The Rhodosporidium toruloides and Lipomyces starkeyi were respectively inserted into 50 mL of activated medium from the test tube, and activated for 24 hours at 20°C to obtain seed liquid. The activated medium was: glucose 20 g / L, peptone 10 g / L, yeast powder 10g / L, the balance is water.

[0042] In the control group, the butanol fermentation wastewater medium did not use polar macroporous adsorption resin for detoxification, and the seed liquid was directly connected to the medium for fermentation.

[0043] The polar macroporous adsorption resin was added to the butanol fermentation wastewater in a proportion of 2 wt%, and stirred for 2 hours by means of screw-type propelling stirring, and the sum of the rotational speed was 100 rpm / min. Resin, take the filtrat...

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Abstract

The invention discloses a method for pretreating alcohol fermentation wastewater to improve the conversion rate of grease yeast and regulate and control the composition of microbial grease fatty acid. The method comprises the following steps: analyzing the removal characteristics of different detoxication agents on a fermentation inhibitor under different conditions; the detoxification conditions are controlled according to different requirements of the grease yeast on the conversion efficiency of alcohol wastewater and fatty acid composition, the process is simple and feasible, the cost is low, the poison of a fermentation inhibitor on the grease yeast can be remarkably reduced, and the production cost is reduced while the conversion efficiency of the grease yeast is improved. According to different removal degrees of fermentation inhibitors in alcohol wastewater by different detoxifiers, the composition of microbial oil fatty acid produced by fermentation of oil yeast is regulated and controlled, and a product rich in highly unsaturated fatty acid and having a higher additional value is obtained.

Description

Technical areas: [0001] The invention relates to a method for pretreating alcohol fermentation wastewater to improve the conversion rate of oil yeast and regulate the fatty acid composition of microbial oil. Background technique: [0002] Ethanol and butanol produced from lignocellulose as raw materials are considered to be the most promising renewable fuels that can replace petroleum. Its production principle is to hydrolyze raw materials rich in lignocellulose through pretreatment and enzymatic hydrolysis into cellulose hydrolyzates rich in available sugars (glucose, xylose, cellobiose, arabinose, etc.), and then ferment and Alcohol is obtained through the distillation process. A large amount of wastewater will be produced during the alcohol fermentation production process, and direct discharge will cause great pollution to the environment. Alcohol fermentation wastewater is rich in organic acid components, fermentation residual sugars, and by-products remaining during th...

Claims

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Application Information

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IPC IPC(8): C12P7/6463C12P39/00C02F1/28C12R1/645C02F103/36
CPCC12P7/6463C12P39/00C02F1/281C02F1/285C02F2103/36
Inventor 陈新德姚时苗熊莲陈雪芳王梦琨
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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