AGEs feed for SPF-level experimental rats and mice and preparation and content determination method of AGEs feed
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A production method, a technology of rats and mice, applied in the direction of animal feed, animal feed, preparation of test samples, etc., can solve complex problems
Pending Publication Date: 2022-07-08
SUZHOU UNIV
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And the feed for rats and mice is a compound food, which is different from the AGEs content detection of a single food mentioned above, which is more complicated than
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Embodiment 1
[0041] The present embodiment provides a preparation method of high AGEs intervention feed:
[0042] The raw material composition includes: 200g casein, 397g corn starch, 132g corn dextrin, 100g sugar, 50g cellulose, 70g soybean oil, 3g cystine, 50g vitamin, 35g S10022M mixed mineral powder and 2.5g choline bitartrate .
[0043]1. Mix casein, corn starch, corn dextrin, sugar, cellulose, soybean oil, methionine, choline, vitamins, and minerals evenly, place them in a single layer, and bake them in an oven for 3 hours. The upper and lower fire temperatures are 130°C. The 32L oven does not exceed 500 grams each time, and a small amount of pure water is sprayed on the surface of the feed every hour to make up for the loss of water caused by baking. (Usually when baking pastries, Maillard reaction occurs when the temperature exceeds 150°C, and the feed of the present invention is mainly made of powders of various grains and proteins, and the moisture content is only 6%. The caram...
Embodiment 2
[0056] Detect the content of various effective components of the AGEs intervention feed obtained in Example 1:
[0057] 1. Apply the FAST fluorescence measurement method to comprehensively evaluate the physicochemical changes of the feed after heat treatment, such as calibration with relevant indicators (such as lysine damage or peroxide level), so as to evaluate the nutritional value of protein in the feed.
[0058] 1) after the feed prepared in Example 1 is ground into fine powder, take 700 mg and dissolve it in 25 ml of 0.1mol / L borate buffer (pH8.2), and filter to obtain filtrate and solid; (using boric acid The protein was isolated and purified by salt buffer and filtered off, some solubilized and some filtered onto cellulose paper);
[0059] 2) Filter the soluble protein on cellulose paper, and measure its FAST index in units of the percentage of AMP (320 / 395nm) and Trp fluorescence (290 / 340nm); FAST index is a sensitive index for evaluating protein thermal damage;
[0...
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Abstract
The invention provides an AGEs feed for SPF-grade experimental rats and mice, and a preparation method and a content determination method of the AGEs feed. The AGEs feed is obtained by the following method: uniformly mixing casein, corn starch, corn dextrin, sugar, cellulose, soybean oil, cystine, vitamins, S10022M mixed mineral powder and choline bitartrate, flatly paving, and uniformly heating; cooling and grinding the substance obtained by heating, and supplementing vitamins and minerals; pressing and shaping the obtained mixture, and performing vacuum packaging; and carrying out sterilization treatment on the packaged product, so as to obtain the AGEs feed. The method also comprises a detection method for testing the content of each nutrient component in the high AGEs feed, and combines a fluorescence and surface fluorescence method and a reverse high performance liquid chromatography method, so that the determination accuracy of the content of AGEs can be improved, and the content of original nutrients in the feed can be evaluated at the same time.
Description
technical field [0001] The invention belongs to the technical field of animal feeding, in particular to an AGEs feed for SPF experimental rats and mice and a method for its preparation and content determination. Background technique [0002] Advanced Glycation End Products (AGEs) are free amino acids and reducing sugars (glucose, fructose, pentose, etc.) of macromolecular substances such as amino acids, proteins, lipids or nucleic acids under non-enzymatic conditions. A group of stable end products produced by the condensation, rearrangement, cleavage, and oxidation modification of aldehyde groups. Divided into endogenous and exogenous two types, of which exogenous / food-derived dietary advanced glycation end products (DietaryAGEs, dAGEs) is one of the main sources of AGEs in the human body, mainly through the Maillard reaction ( Also known as non-enzymatic browning reaction, it is a kind of non-enzymatic browning widely present in the food industry. After a complex process,...
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