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Method for reducing and fixing Cr (VI) by using electron-mediated polydopamine modified shewanella

A technology based on Shewanella and polydopamine, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of difficult treatment of chromium element returning to yellow chromium wastewater, and achieve large-scale continuous The effect of industrialized chemical production, large processing capacity and high economic benefits

Pending Publication Date: 2022-08-02
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem that the yellowing of chromium element and low-concentration chromium wastewater are not easy to handle in the treatment of chromium-containing wastewater in the prior art, the object of the present invention is to provide a kind of electron-mediated polydopamine modified Shewanella to reduce and fix Cr(VI )Methods

Method used

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  • Method for reducing and fixing Cr (VI) by using electron-mediated polydopamine modified shewanella
  • Method for reducing and fixing Cr (VI) by using electron-mediated polydopamine modified shewanella
  • Method for reducing and fixing Cr (VI) by using electron-mediated polydopamine modified shewanella

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Embodiment 1

[0042] Polydopamine surface and interior modification Shewanella: inoculation with Shewanella oneidensis MR-1 strain (this strain is related to the article Carbon dots-fed Shewanella oneidensis MR-1 for bioelectricity enhancement. (https: / / doi.org / 10.1038 / s41467-020-14866-0) were the same strains) into LB medium, and aerobic cultivation was carried out in a constant temperature environment of 30°C. when OD 600 When reaching 1.5, the cells (cells) were collected by centrifugation, and washed several times with 0.85% NaCl aqueous solution and Tris-HCl buffer. Immerse the collected cells in Tris-HCl buffer to OD 600 1.5 was reached and dopamine hydrochloride was added to the cell suspension at concentrations of 4, 8, 12 and 16 mg / mL, respectively. The Shewanella / dopamine mixed solution was shaken aerobically at 37°C for 3 hours. Polydopamine-modified Shewanella (PDA@S.oneidensis MR-1(1)) were collected by centrifugation and washed several times with nitrogen-purged distilled ...

Embodiment 2

[0046] Construction of bioanode: The polydopamine-modified Shewanella in Example 1 was used as the experimental conductive strain of this system (the concentration of dopamine hydrochloride was 8 mg / mL, and the reaction was performed at 37° C. for 3 h). A three-electrode microbial electrochemical system was constructed, wherein the three-electrode system was composed of a working electrode, a reference electrode and a counter electrode. A wire was used to connect the three-electrode microbial system to a multi-channel potentiostat, which applied a positive potential (+200 mV). Conductive bacteria (OD 600 = 1) inoculated into anolyte (consisting of phosphate buffered solution, vitamins, trace minerals and C 3 H 5 O 3 Na composition, the concentration of vitamins in the anolyte is 10mL / L, the concentration of trace minerals is 12.5mL / L, C 3 H 5 O 3 The concentration of Na is 18mmol / L; the phosphate buffer solution is composed of Na 2 HPO 4 ·2H 2 O 3.32g / L, NaH 2 PO 4 ...

Embodiment 3

[0050] The biofilm and composite film of the anode conductive network were reversed to the cathode (the construction of a ternary system of electron-biofilm / composite film-Cr(VI)): In a three-electrode bioelectrochemical system, the chromium resistance in Example 2 was acclimated After the bioanode is placed in the catholyte (consisting of phosphate buffered solution, vitamins, trace minerals and NaHCO) 3 Composition, the concentration of vitamins in the catholyte is 10mL / L, the concentration of trace minerals is 12.5mL / L, the concentration of NaHCO 3 The concentration of 18mmol / L; the phosphate buffer solution is composed of Na 2 HPO 4 ·2H 2 O 3.32g / L, NaH 2 PO 4 ·12H 2 O 10.32g / L, NH 4 Composition of Cl 0.31g / L and KCl 0.13g / L, pH=7.0; trace minerals by 3.0g MgSO 4 ·7H 2 O, 0.5g MnSO 4 ·H 2 O, 1.0g NaCl, 0.1g FeSO 4 ·7H 2 O, 0.1g CoCl 2 ·6H 2 O, 0.1g CaCl 2 , 0.1g ZnSO 4 ·7H2 O, 0.01g CuSO 4 ·5H 2 O, 0.1g AKl (SO 4 ) 2 ·12H 2 O, 0.01g H 3 BO 3 and 0.01...

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Abstract

The invention discloses a method for reducing and fixing Cr (VI) by using electron-mediated polydopamine modified shewanella. According to the method, shewanella is subjected to polydopamine modification, then the modified shewanella is subjected to anode domestication by using a three-electrode microbial electrochemical system to obtain a biological membrane, and then the biological membrane is subjected to cathode domestication, so that the reduction and immobilization of Cr (VI) in the catholyte can be realized. According to the method disclosed by the invention, functional modification of nano-particles on the surface and inside of shewanella is realized, intracellular and extracellular electron transfer and metabolism rates are synergistically accelerated, an electron-biological membrane-Cr (VI) ternary system is constructed, and reduction immobilization of Cr (VI) is realized by utilizing a BESs technology and microorganism surface organic functional groups on a conductive network composite membrane; the method is simple in equipment and high in operability, has the potential of large-scale continuous industrial production, and is expected to solve the current environmental problem.

Description

technical field [0001] The invention belongs to the technical field of harmless hazardous waste, in particular to a method for reducing and immobilizing Cr(VI) by polydopamine-modified Shewanella under the mediation of electrons. Background technique [0002] Chromium and its compounds are widely used in steel, electroplating, tanning, fuel and other industries, so chromium has become one of the main pollutants of heavy metal pollution in soil. Chromium is a non-degradable toxic substance. At present, the main idea of ​​soil chromium pollution treatment is to reduce the mobility of chromium in soil by reducing the highly toxic hexavalent chromium to low toxicity trivalent chromium and changing its existing form. The traditional methods of soil chromium pollution control mainly include physical method and chemical method. With the development of biotechnology, microbial remediation has gradually been applied to practice from the experimental stage, which has the advantages o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/00C02F3/34C12R1/01C02F101/22
CPCC12N1/20C12N1/005C02F3/005C02F3/34C12R2001/01C02F2101/22Y02E60/50
Inventor 张丽娟贾博宇宿新泰刘天宝李玮佳向宇佳
Owner SOUTH CHINA UNIV OF TECH
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