Method for preparing polypeptide by utilizing eggshell membrane, polypeptide prepared by method and application of polypeptide

An eggshell film and facial mask technology, applied in the field of peptides and applications, can solve the problems of low efficiency and time-consuming enzymatic hydrolysis, and achieve the effects of high efficiency, easy skin absorption, and small molecular weight

Pending Publication Date: 2022-08-02
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, most of the research on eggshell membrane proteins and polypeptides is to prepare eggshell membrane hydrolyzate by enzymatic hydrolysis, and then conduct research on antioxidant activity. Enzymatic hydrolysis of eggshell membrane has mild reaction conditions, stable physical and chemical properties, and no environmental pollution. It is non-toxic to the human body and is currently a commonly used method, but the enzymatic hydrolysis method takes a long time and is inefficient

Method used

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  • Method for preparing polypeptide by utilizing eggshell membrane, polypeptide prepared by method and application of polypeptide
  • Method for preparing polypeptide by utilizing eggshell membrane, polypeptide prepared by method and application of polypeptide
  • Method for preparing polypeptide by utilizing eggshell membrane, polypeptide prepared by method and application of polypeptide

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preparation example Construction

[0053] The present invention also provides the preparation method of the above-mentioned facial mask, is characterized in that, comprises the steps:

[0054] S11, prepare each component according to the mass percentage;

[0055] S12, take the poloxamer 407 and part of the water to configure the poloxamer 407 aqueous solution, take the polyvinyl alcohol and the remaining water to configure the polyvinyl alcohol solution, and mix the poloxamer 407 aqueous solution and the polyvinyl alcohol solution in the Mix uniformly at 1-6° C., then add the glycerin and mix uniformly, and then add the polypeptide and mix uniformly to obtain the facial mask.

[0056] Based on the preparation method of the mask according to the embodiment of the present invention, the preparation process of the Poloxamer 407 aqueous solution is as follows: after mixing the Poloxamer 407 with the part of the water evenly, it is placed at 1-6° C. overnight to fully swell .

[0057] Based on the preparation meth...

Embodiment 1

[0059] (1) take by weighing 300 grams of eggshell membranes and place them in the steam explosion machine reactor, and under the pressure of 1.2MPa, hold the pressure for 3min, then release the pressure instantly, collect the steam explosion samples, and store at -20 ℃ for subsequent use;

[0060] (2) Weigh 3 grams of eggshell membranes processed by steam explosion in a 100mL conical flask, add 60mL of an aqueous sodium hydroxide solution with a pH of 8.5 at a material-to-liquid ratio of 1:20, add an enzyme amount of 6000U / g Alkaline protease was used for enzymatic hydrolysis. The enzymatic hydrolysis time was 5 h, and the enzymatic hydrolysis temperature was 56 °C. After the enzymatic hydrolysis reaction, the enzyme was inactivated at 90 °C for 10 min. The enzymatic hydrolysis solution was filtered, and the filtrate was centrifuged at 8000 rpm for 10 min. The shell membrane polypeptide product was obtained by drying and stored at -20°C for later use.

[0061] (3) Prepare an a...

Embodiment 2

[0064] (1) take by weighing 300 grams of eggshell membranes and place them in the reactor of a steam explosion machine, under the pressure of 1.4MPa, hold the pressure for 3min, then release the pressure instantaneously, collect the steam explosion samples, and store at -20 ℃ for subsequent use;

[0065] (2) Weigh 3 grams of eggshell membranes treated by steam explosion in a 100mL conical flask, add 60mL of an aqueous sodium hydroxide solution with a pH of 7 at a material-to-liquid ratio of 1:30, add an enzyme amount of 2000U / g Alkaline protease was used for enzymatic hydrolysis. The enzymatic hydrolysis time was 2 h, and the enzymatic hydrolysis temperature was 35 °C. After the enzymatic hydrolysis reaction was completed, the enzyme was inactivated at 90 °C for 10 min. The shell membrane polypeptide product was obtained by drying and stored at -20°C for later use.

[0066] (3) First, prepare the poloxamer 407 aqueous solution with a mass fraction of 14%, place it in a refrige...

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Abstract

The method comprises the following steps: S1, steam explosion treatment: placing the eggshell membrane in a steam explosion machine reactor, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.4 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; and S2, protease enzymolysis treatment: adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-10 according to the solid-liquid ratio of 1: (10-30), then adding alkaline protease according to the enzyme addition amount of 2000-14000 U/g, carrying out enzymolysis for 2-14 hours at the temperature of 35-65 DEG C, carrying out enzyme deactivation for 5-15 minutes at the temperature of 90-100 DEG C, filtering enzymatic hydrolysate, taking filtrate for centrifugation, taking supernate, and carrying out freeze drying to obtain the polypeptide. Based on the method, the preparation efficiency is high, and the prepared polypeptide is high in antioxidant activity.

Description

technical field [0001] The invention belongs to the field of egg by-product processing, and in particular relates to a method for preparing polypeptides by using eggshell membranes, and the prepared polypeptides and applications. Background technique [0002] my country is the country with the most poultry in the world. With the increasing production and consumption of eggs, egg shells are also produced in large quantities and discarded, which not only causes serious pollution to the environment, but also wastes resources. Eggshell membrane is a thin film located on the inner surface of the eggshell, with a thickness of about 100 μm, mainly composed of proteins such as keratin, salivary protein, osteopontin and collagen (including type I, V and X), etc. Contains hyaluronic acid, chondroitin sulfate and other biologically active substances, which can be widely used in the pharmaceutical and cosmetic industries. However, eggshell membrane is composed of two layers of dense pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K8/81A61K8/86A61K8/368A61Q19/08
CPCC12P21/06A61K8/64A61K8/8129A61K8/86A61K8/368A61K8/0212A61Q19/08A61K2800/592Y02P20/54
Inventor 黄茜张源超唐翠娥宁文静蔡朝霞
Owner HUAZHONG AGRI UNIV
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